miR-146a expression and the miR-146a gene variant rs2910164 as diagnostic and prognostic biomarkers of oral cancer
This study aimed to analyze the diagnostic and prognostic significance of miR-146a-5p in oral cancer and the association of the rs2910164 gene variant with miR-146a-5p expression. Thirty-five oral cancer patients participated in the study. Expression of miR-146a-5p in oral cancer, adjacent non-cance...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2024-01-01
|
| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400032V.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832581986342928384 |
|---|---|
| author | Vuković Miodrag Stojković Goran Zeljić Katarina |
| author_facet | Vuković Miodrag Stojković Goran Zeljić Katarina |
| author_sort | Vuković Miodrag |
| collection | DOAJ |
| description | This study aimed to analyze the diagnostic and prognostic significance of miR-146a-5p in oral cancer and the association of the rs2910164 gene variant with miR-146a-5p expression. Thirty-five oral cancer patients participated in the study. Expression of miR-146a-5p in oral cancer, adjacent non-cancerous tissue, and the rs2910164 genotype variant were examined by real-time PCR. No difference was observed in miR-146a-5p expression between cancerous and non-cancerous tissues (P=0.272). It was concluded that miR-146a-5p is not a good diagnostic biomarker. There was no difference in miR- 146a-5p expression depending on the rs2910164 genotype. The rs2910164 variant was associated with tumor histological grade (P=0.037), stage (P=0.036), and lymph node status (P=0.025). There was a difference in survival between oral cancer patients with high and low miR-146a-5p expression (P=0.026), but not between patients with the GG and GC genotypes (P=0.400). Oral cancer patients with the wild-type GG genotype and high miR-146a-5p expression had significantly shorter survival when compared to patients with miR-146a-5p expression below the optimal cut-off and the wild-type genotype (P=0.035). A high miR-146a-5p level could be considered a potential biomarker of poor survival in patients with oral cancer, especially in those with the wild-type GG genotype of the rs2910164 variant. |
| format | Article |
| id | doaj-art-cce50b3520c34649852da31fab09747c |
| institution | Kabale University |
| issn | 0354-4664 1821-4339 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj-art-cce50b3520c34649852da31fab09747c2025-01-30T06:38:39ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392024-01-0176441942910.2298/ABS240711032V0354-46642400032VmiR-146a expression and the miR-146a gene variant rs2910164 as diagnostic and prognostic biomarkers of oral cancerVuković Miodrag0https://orcid.org/0000-0002-9485-6488Stojković Goran1https://orcid.org/0000-0003-3659-6136Zeljić Katarina2https://orcid.org/0000-0002-6545-8616Faculty of Biology, University of Belgrade, Belgrade, Serbia + Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Belgrade, SerbiaClinic for Otorhinolaryngology and Maxillofacial Surgery, University Clinical Center Serbia, Belgrade, Serbia + Faculty of Medicine, University of Belgrade, Belgrade, SerbiaFaculty of Biology, University of Belgrade, Belgrade, SerbiaThis study aimed to analyze the diagnostic and prognostic significance of miR-146a-5p in oral cancer and the association of the rs2910164 gene variant with miR-146a-5p expression. Thirty-five oral cancer patients participated in the study. Expression of miR-146a-5p in oral cancer, adjacent non-cancerous tissue, and the rs2910164 genotype variant were examined by real-time PCR. No difference was observed in miR-146a-5p expression between cancerous and non-cancerous tissues (P=0.272). It was concluded that miR-146a-5p is not a good diagnostic biomarker. There was no difference in miR- 146a-5p expression depending on the rs2910164 genotype. The rs2910164 variant was associated with tumor histological grade (P=0.037), stage (P=0.036), and lymph node status (P=0.025). There was a difference in survival between oral cancer patients with high and low miR-146a-5p expression (P=0.026), but not between patients with the GG and GC genotypes (P=0.400). Oral cancer patients with the wild-type GG genotype and high miR-146a-5p expression had significantly shorter survival when compared to patients with miR-146a-5p expression below the optimal cut-off and the wild-type genotype (P=0.035). A high miR-146a-5p level could be considered a potential biomarker of poor survival in patients with oral cancer, especially in those with the wild-type GG genotype of the rs2910164 variant.https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400032V.pdforal cancermir-146a-5prs2910164biomarker |
| spellingShingle | Vuković Miodrag Stojković Goran Zeljić Katarina miR-146a expression and the miR-146a gene variant rs2910164 as diagnostic and prognostic biomarkers of oral cancer Archives of Biological Sciences oral cancer mir-146a-5p rs2910164 biomarker |
| title | miR-146a expression and the miR-146a gene variant rs2910164 as diagnostic and prognostic biomarkers of oral cancer |
| title_full | miR-146a expression and the miR-146a gene variant rs2910164 as diagnostic and prognostic biomarkers of oral cancer |
| title_fullStr | miR-146a expression and the miR-146a gene variant rs2910164 as diagnostic and prognostic biomarkers of oral cancer |
| title_full_unstemmed | miR-146a expression and the miR-146a gene variant rs2910164 as diagnostic and prognostic biomarkers of oral cancer |
| title_short | miR-146a expression and the miR-146a gene variant rs2910164 as diagnostic and prognostic biomarkers of oral cancer |
| title_sort | mir 146a expression and the mir 146a gene variant rs2910164 as diagnostic and prognostic biomarkers of oral cancer |
| topic | oral cancer mir-146a-5p rs2910164 biomarker |
| url | https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400032V.pdf |
| work_keys_str_mv | AT vukovicmiodrag mir146aexpressionandthemir146agenevariantrs2910164asdiagnosticandprognosticbiomarkersoforalcancer AT stojkovicgoran mir146aexpressionandthemir146agenevariantrs2910164asdiagnosticandprognosticbiomarkersoforalcancer AT zeljickatarina mir146aexpressionandthemir146agenevariantrs2910164asdiagnosticandprognosticbiomarkersoforalcancer |