Peptide P2 targeting Vibrio parahaemolyticus PirB toxins blocks the cytotoxic effects to shrimp Litopenaeus vannamei
PirB is the main virulence factor of acute hepatopancreatic necrosis disease (AHPND) which induced the sloughing of hepatopancreatic cells and shrimp death. In addition, Litopenaeus vannamei aminopeptidase N (Lv-APN) is reported to be a functional receptor that mediates the pathogenicity by VpAHPND....
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006525 |
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| author | Xin Zhang Ting Chen Chunhua Ren Yanhong Wang Xiao Jiang Jiasheng Huang Bo Ma Boxiang Zhu Jiayue Yin Chaoqun Hu Peng Luo |
| author_facet | Xin Zhang Ting Chen Chunhua Ren Yanhong Wang Xiao Jiang Jiasheng Huang Bo Ma Boxiang Zhu Jiayue Yin Chaoqun Hu Peng Luo |
| author_sort | Xin Zhang |
| collection | DOAJ |
| description | PirB is the main virulence factor of acute hepatopancreatic necrosis disease (AHPND) which induced the sloughing of hepatopancreatic cells and shrimp death. In addition, Litopenaeus vannamei aminopeptidase N (Lv-APN) is reported to be a functional receptor that mediates the pathogenicity by VpAHPND. This study aims to screen the PirB binding peptide to rescue the survival rate under VpAHPND infection. Here, the PirB toxin-binding peptides were selected using the random phage peptide library kit, and their biological function was verified by injecting them under VpAHPND challenge. The receptor candidates for PirB were genome-wide identified and selected based on gene expression profiles, and the biological function of Lv-APN1 was confirmed through RNAi. Docking analysis of Peptide-Lv-APN1 and PirB-Lv-APN1 was conducted using the MDockPeP server, with comparative analysis implied. As a result, a total of 11 PirB binding peptides were screened, among which P2 was found to effectively improve the shrimp survival rate under VpAHPND challenge. And knocked down of Lv-APN1, candidate receptor for PirB toxin, rescued mortality under VpAHPND challenge. Furthermore, docking analysis revealed that the interface of Lv-APN1 to rPirB was consistent with the P2 to rPirB, suggesting that P2 is likely to bind to PirB to block its binding to Lv-APN1 to reduce the mortality. In conclusion, both the injection of P2 and inference of Lv-APN1 can rescue the mortality of shrimp under VpAHPND challenge, and docking analysis revealed P2 is likely to bind to rPirB, blocking its binding to Lv-APN1 and reducing VpAHPND infection. |
| format | Article |
| id | doaj-art-ccccb71d832946b3a5eba7f2a10ef162 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-ccccb71d832946b3a5eba7f2a10ef1622025-02-06T05:12:09ZengElsevierAquaculture Reports2352-51342025-03-0140102564Peptide P2 targeting Vibrio parahaemolyticus PirB toxins blocks the cytotoxic effects to shrimp Litopenaeus vannameiXin Zhang0Ting Chen1Chunhua Ren2Yanhong Wang3Xiao Jiang4Jiasheng Huang5Bo Ma6Boxiang Zhu7Jiayue Yin8Chaoqun Hu9Peng Luo10Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaKey Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaKey Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaKey Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaKey Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaKey Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; University of Chinese Academy of Sciences, Beijing, PR ChinaKey Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; University of Chinese Academy of Sciences, Beijing, PR ChinaShanghai Collaborative Innovation Centre for Aquatic Animal Genetics and Breeding, research Centre on Aquaculture Nutrition and Environmental Ecology of the Ministry of Agriculture and Rural Affair, Shanghai Ocean University, Shanghai, PR ChinaKey Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; University of Chinese Academy of Sciences, Beijing, PR ChinaKey Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; Corresponding authors.Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; Corresponding authors.PirB is the main virulence factor of acute hepatopancreatic necrosis disease (AHPND) which induced the sloughing of hepatopancreatic cells and shrimp death. In addition, Litopenaeus vannamei aminopeptidase N (Lv-APN) is reported to be a functional receptor that mediates the pathogenicity by VpAHPND. This study aims to screen the PirB binding peptide to rescue the survival rate under VpAHPND infection. Here, the PirB toxin-binding peptides were selected using the random phage peptide library kit, and their biological function was verified by injecting them under VpAHPND challenge. The receptor candidates for PirB were genome-wide identified and selected based on gene expression profiles, and the biological function of Lv-APN1 was confirmed through RNAi. Docking analysis of Peptide-Lv-APN1 and PirB-Lv-APN1 was conducted using the MDockPeP server, with comparative analysis implied. As a result, a total of 11 PirB binding peptides were screened, among which P2 was found to effectively improve the shrimp survival rate under VpAHPND challenge. And knocked down of Lv-APN1, candidate receptor for PirB toxin, rescued mortality under VpAHPND challenge. Furthermore, docking analysis revealed that the interface of Lv-APN1 to rPirB was consistent with the P2 to rPirB, suggesting that P2 is likely to bind to PirB to block its binding to Lv-APN1 to reduce the mortality. In conclusion, both the injection of P2 and inference of Lv-APN1 can rescue the mortality of shrimp under VpAHPND challenge, and docking analysis revealed P2 is likely to bind to rPirB, blocking its binding to Lv-APN1 and reducing VpAHPND infection.http://www.sciencedirect.com/science/article/pii/S2352513424006525Litopenaeus vannameiAHPNDPir BBinding peptideAPN |
| spellingShingle | Xin Zhang Ting Chen Chunhua Ren Yanhong Wang Xiao Jiang Jiasheng Huang Bo Ma Boxiang Zhu Jiayue Yin Chaoqun Hu Peng Luo Peptide P2 targeting Vibrio parahaemolyticus PirB toxins blocks the cytotoxic effects to shrimp Litopenaeus vannamei Aquaculture Reports Litopenaeus vannamei AHPND Pir B Binding peptide APN |
| title | Peptide P2 targeting Vibrio parahaemolyticus PirB toxins blocks the cytotoxic effects to shrimp Litopenaeus vannamei |
| title_full | Peptide P2 targeting Vibrio parahaemolyticus PirB toxins blocks the cytotoxic effects to shrimp Litopenaeus vannamei |
| title_fullStr | Peptide P2 targeting Vibrio parahaemolyticus PirB toxins blocks the cytotoxic effects to shrimp Litopenaeus vannamei |
| title_full_unstemmed | Peptide P2 targeting Vibrio parahaemolyticus PirB toxins blocks the cytotoxic effects to shrimp Litopenaeus vannamei |
| title_short | Peptide P2 targeting Vibrio parahaemolyticus PirB toxins blocks the cytotoxic effects to shrimp Litopenaeus vannamei |
| title_sort | peptide p2 targeting vibrio parahaemolyticus pirb toxins blocks the cytotoxic effects to shrimp litopenaeus vannamei |
| topic | Litopenaeus vannamei AHPND Pir B Binding peptide APN |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006525 |
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