Roquefort Cheese Proteins Inhibit Chlamydia pneumoniae Propagation and LPS-Induced Leukocyte Migration
Inflammation in atherosclerosis, which could be associated with some subclinical infections such as C. pneumoniae, is one of the key factors responsible for the development of clinical complications of this disease. We report that a proprietary protein extract isolated from Roquefort cheese inhibits...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/140591 |
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| author | Ivan M. Petyaev Naylia A. Zigangirova Natalie V. Kobets Valery Tsibezov Lydia N. Kapotina Elena D. Fedina Yuriy K. Bashmakov |
| author_facet | Ivan M. Petyaev Naylia A. Zigangirova Natalie V. Kobets Valery Tsibezov Lydia N. Kapotina Elena D. Fedina Yuriy K. Bashmakov |
| author_sort | Ivan M. Petyaev |
| collection | DOAJ |
| description | Inflammation in atherosclerosis, which could be associated with some subclinical infections such as C. pneumoniae, is one of the key factors responsible for the development of clinical complications of this disease. We report that a proprietary protein extract isolated from Roquefort cheese inhibits the propagation of C. pneumoniae in a human HL cell line in a dose-dependent manner, as revealed by the immunofluorescence analysis. These changes were accompanied by a significant reduction in the infective progeny formation over the protein extract range of 0.12–0.5 μg/mL. Moreover, short term feeding of mice with Roquefort cheese (twice, 10 mg per mouse with an interval of 24 hours) led to the inhibition of the migration of peritoneal leukocytes caused by intraperitoneal injection of E. coli lipopolysaccharide. These changes were complemented by a reduction in neutrophil count and a relative increase in peritoneal macrophages, suggesting that ingestion of Roquefort could promote regenerative processes at the site of inflammation. The ability of this protein to inhibit propagation of Chlamydia infection, as well as the anti-inflammatory and proregenerative effects of Roquefort itself, may contribute to the low prevalence of cardiovascular mortality in France where consumption of fungal fermented cheeses is the highest in the world. |
| format | Article |
| id | doaj-art-ccbd1bb559fc41f88e5b72408b14297f |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-ccbd1bb559fc41f88e5b72408b14297f2025-02-03T05:46:08ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/140591140591Roquefort Cheese Proteins Inhibit Chlamydia pneumoniae Propagation and LPS-Induced Leukocyte MigrationIvan M. Petyaev0Naylia A. Zigangirova1Natalie V. Kobets2Valery Tsibezov3Lydia N. Kapotina4Elena D. Fedina5Yuriy K. Bashmakov6Lycotec Ltd. Granta Park Campus, Cambridge CB21 6GP, UKGamaleya Institute of Epidemiology and Microbiology, Ministry of Health, 18 Gamaleya Street, Moscow 123098, RussiaGamaleya Institute of Epidemiology and Microbiology, Ministry of Health, 18 Gamaleya Street, Moscow 123098, RussiaGamaleya Institute of Epidemiology and Microbiology, Ministry of Health, 18 Gamaleya Street, Moscow 123098, RussiaGamaleya Institute of Epidemiology and Microbiology, Ministry of Health, 18 Gamaleya Street, Moscow 123098, RussiaGamaleya Institute of Epidemiology and Microbiology, Ministry of Health, 18 Gamaleya Street, Moscow 123098, RussiaLycotec Ltd. Granta Park Campus, Cambridge CB21 6GP, UKInflammation in atherosclerosis, which could be associated with some subclinical infections such as C. pneumoniae, is one of the key factors responsible for the development of clinical complications of this disease. We report that a proprietary protein extract isolated from Roquefort cheese inhibits the propagation of C. pneumoniae in a human HL cell line in a dose-dependent manner, as revealed by the immunofluorescence analysis. These changes were accompanied by a significant reduction in the infective progeny formation over the protein extract range of 0.12–0.5 μg/mL. Moreover, short term feeding of mice with Roquefort cheese (twice, 10 mg per mouse with an interval of 24 hours) led to the inhibition of the migration of peritoneal leukocytes caused by intraperitoneal injection of E. coli lipopolysaccharide. These changes were complemented by a reduction in neutrophil count and a relative increase in peritoneal macrophages, suggesting that ingestion of Roquefort could promote regenerative processes at the site of inflammation. The ability of this protein to inhibit propagation of Chlamydia infection, as well as the anti-inflammatory and proregenerative effects of Roquefort itself, may contribute to the low prevalence of cardiovascular mortality in France where consumption of fungal fermented cheeses is the highest in the world.http://dx.doi.org/10.1155/2013/140591 |
| spellingShingle | Ivan M. Petyaev Naylia A. Zigangirova Natalie V. Kobets Valery Tsibezov Lydia N. Kapotina Elena D. Fedina Yuriy K. Bashmakov Roquefort Cheese Proteins Inhibit Chlamydia pneumoniae Propagation and LPS-Induced Leukocyte Migration The Scientific World Journal |
| title | Roquefort Cheese Proteins Inhibit Chlamydia pneumoniae Propagation and LPS-Induced Leukocyte Migration |
| title_full | Roquefort Cheese Proteins Inhibit Chlamydia pneumoniae Propagation and LPS-Induced Leukocyte Migration |
| title_fullStr | Roquefort Cheese Proteins Inhibit Chlamydia pneumoniae Propagation and LPS-Induced Leukocyte Migration |
| title_full_unstemmed | Roquefort Cheese Proteins Inhibit Chlamydia pneumoniae Propagation and LPS-Induced Leukocyte Migration |
| title_short | Roquefort Cheese Proteins Inhibit Chlamydia pneumoniae Propagation and LPS-Induced Leukocyte Migration |
| title_sort | roquefort cheese proteins inhibit chlamydia pneumoniae propagation and lps induced leukocyte migration |
| url | http://dx.doi.org/10.1155/2013/140591 |
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