Escherichia coli O157:H7 Bacteria Antibiotic Resistant Isolated from Flies at Food Courts in IPB Dramaga Campus
Several human and animal pathogens transmit into the food chain through houseflies as mechanical vectors, one of which is E. coli O157:H7. E. coli O157:H7 can express Shiga toxin (Stx) which can cause diarrhea, hemorrhagic colitis, and potentially fatal hemolytic-uremic syndrome (HUS) in humans. Som...
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| Format: | Article |
| Language: | Indonesian |
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Universitas Gadjah Mada
2024-12-01
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| Series: | Jurnal Sain Veteriner |
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| Online Access: | https://jurnal.ugm.ac.id/jsv/article/view/99843 |
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| author | Muhammad Rizki Aminudin Denny Widaya Lukman Mirnawati Bachrum Sudarwanto Herwin Pisestyani |
| author_facet | Muhammad Rizki Aminudin Denny Widaya Lukman Mirnawati Bachrum Sudarwanto Herwin Pisestyani |
| author_sort | Muhammad Rizki Aminudin |
| collection | DOAJ |
| description | Several human and animal pathogens transmit into the food chain through houseflies as mechanical vectors, one of which is E. coli O157:H7. E. coli O157:H7 can express Shiga toxin (Stx) which can cause diarrhea, hemorrhagic colitis, and potentially fatal hemolytic-uremic syndrome (HUS) in humans. Some pathogen strains show resistance against various antibiotics, causing complex health problems. This study aims to analyze the presence of antibiotic-resistant E. coli O157:H7 bacteria carried by houseflies (M. domestica) in the food court IPB Dramaga campus area. Detection of E. coli O157:H7 on fly legs using qPCR method based on MU 7.2.3.32-8. E. coli O157:H7 isolates were tested for sensitivity to the antibiotic’s ciprofloxacin, ampicillin, tetracycline, trimethoprim-sulfamethoxazole, cefepime, chloramphenicol, ceftazidime, cefotaxime, and ceftriaxone using the Kirby Bauer disc diffusion method. This study isolated 5 E. coli isolates (5/40; 12.5%), and 2 of them were confirmed as E. coli O157:H7. One isolate of E. coli O157:H7 was resistant against ampicillin and tetracycline, and one isolate was resistant against ampicillin, tetracycline, trimethoprim-sulfamethoxazole, and ceftazidime. The multi-drug resistance was identified only in 1 isolate of E. coli O157:H7.Houseflies collected from the food court have the potential to transmit antibiotic-resistant E. coli O157:H7 around the IPB campus. |
| format | Article |
| id | doaj-art-cc62a3ac843144479d8644f6c45d5599 |
| institution | Kabale University |
| issn | 2407-3733 |
| language | Indonesian |
| publishDate | 2024-12-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Jurnal Sain Veteriner |
| spelling | doaj-art-cc62a3ac843144479d8644f6c45d55992025-01-20T02:12:39ZindUniversitas Gadjah MadaJurnal Sain Veteriner2407-37332024-12-0142334535510.22146/jsv.9984337051Escherichia coli O157:H7 Bacteria Antibiotic Resistant Isolated from Flies at Food Courts in IPB Dramaga CampusMuhammad Rizki Aminudin0Denny Widaya Lukman1Mirnawati Bachrum Sudarwanto2Herwin Pisestyani3Program Pascasarjana, Sekolah Kedokteran Hewan dan Biomedis, IPB UniversityDivisi Kesehatan Masyarakat Veteriner dan Epidemiologi, Sekolah Kedokteran Hewan dan Biomedis, IPB UniversityDivisi Kesehatan Masyarakat Veteriner dan Epidemiologi, Sekolah Kedokteran Hewan dan Biomedis, IPB UniversityDivisi Kesehatan Masyarakat Veteriner dan Epidemiologi, Sekolah Kedokteran Hewan dan Biomedis, IPB UniversitySeveral human and animal pathogens transmit into the food chain through houseflies as mechanical vectors, one of which is E. coli O157:H7. E. coli O157:H7 can express Shiga toxin (Stx) which can cause diarrhea, hemorrhagic colitis, and potentially fatal hemolytic-uremic syndrome (HUS) in humans. Some pathogen strains show resistance against various antibiotics, causing complex health problems. This study aims to analyze the presence of antibiotic-resistant E. coli O157:H7 bacteria carried by houseflies (M. domestica) in the food court IPB Dramaga campus area. Detection of E. coli O157:H7 on fly legs using qPCR method based on MU 7.2.3.32-8. E. coli O157:H7 isolates were tested for sensitivity to the antibiotic’s ciprofloxacin, ampicillin, tetracycline, trimethoprim-sulfamethoxazole, cefepime, chloramphenicol, ceftazidime, cefotaxime, and ceftriaxone using the Kirby Bauer disc diffusion method. This study isolated 5 E. coli isolates (5/40; 12.5%), and 2 of them were confirmed as E. coli O157:H7. One isolate of E. coli O157:H7 was resistant against ampicillin and tetracycline, and one isolate was resistant against ampicillin, tetracycline, trimethoprim-sulfamethoxazole, and ceftazidime. The multi-drug resistance was identified only in 1 isolate of E. coli O157:H7.Houseflies collected from the food court have the potential to transmit antibiotic-resistant E. coli O157:H7 around the IPB campus.https://jurnal.ugm.ac.id/jsv/article/view/99843escherichia coli o157:h7houseflymulti-drug resistance |
| spellingShingle | Muhammad Rizki Aminudin Denny Widaya Lukman Mirnawati Bachrum Sudarwanto Herwin Pisestyani Escherichia coli O157:H7 Bacteria Antibiotic Resistant Isolated from Flies at Food Courts in IPB Dramaga Campus Jurnal Sain Veteriner escherichia coli o157:h7 housefly multi-drug resistance |
| title | Escherichia coli O157:H7 Bacteria Antibiotic Resistant Isolated from Flies at Food Courts in IPB Dramaga Campus |
| title_full | Escherichia coli O157:H7 Bacteria Antibiotic Resistant Isolated from Flies at Food Courts in IPB Dramaga Campus |
| title_fullStr | Escherichia coli O157:H7 Bacteria Antibiotic Resistant Isolated from Flies at Food Courts in IPB Dramaga Campus |
| title_full_unstemmed | Escherichia coli O157:H7 Bacteria Antibiotic Resistant Isolated from Flies at Food Courts in IPB Dramaga Campus |
| title_short | Escherichia coli O157:H7 Bacteria Antibiotic Resistant Isolated from Flies at Food Courts in IPB Dramaga Campus |
| title_sort | escherichia coli o157 h7 bacteria antibiotic resistant isolated from flies at food courts in ipb dramaga campus |
| topic | escherichia coli o157:h7 housefly multi-drug resistance |
| url | https://jurnal.ugm.ac.id/jsv/article/view/99843 |
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