Osteoarthritis synovium as a nidus for monosodium urate crystal deposition inducing severe gout studied by label‐free stimulated Raman scattering combined with synovial organoids
Abstract Gout, a common chronic disease, is characterized by the formation and deposition of monosodium urate (MSU) crystal deposition in articular and nonarticular structures. Osteoarthritis (OA), the most prevalent type of arthritis, is a progressive degenerative joint disease. Previous clinical s...
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Wiley
2025-01-01
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| Online Access: | https://doi.org/10.1002/mco2.70040 |
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| author | Ziyi Chen Wenjuan Wang Yaxin Chen Minbiao Ji Yinghui Hua |
| author_facet | Ziyi Chen Wenjuan Wang Yaxin Chen Minbiao Ji Yinghui Hua |
| author_sort | Ziyi Chen |
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| description | Abstract Gout, a common chronic disease, is characterized by the formation and deposition of monosodium urate (MSU) crystal deposition in articular and nonarticular structures. Osteoarthritis (OA), the most prevalent type of arthritis, is a progressive degenerative joint disease. Previous clinical studies have reported that gout frequently affects OA joints; however, the underlying mechanism remains unidentified. Recently, OA synovium has been proposed as a favorable vehicle for MSU crystal deposition. Therefore, this study aimed to investigate whether OA synovium acts as a nidus for MSU crystal deposition inducing severe gout flares, using label‐free, highly‐specific stimulated Raman scattering (SRS) microscopy combined with innovative preclinical models—synovial organoids. Crystal deposition, cellular phagocytosis, and subsequent inflammation intensity was imaged in ex vivo synovial organoids using SRS microscopy and other biochemical techniques. Results revealed that MSU crystals were more likely to deposit in OA synovium than in normal synovium. Furthermore, OA synoviocytes were more capable of phagocytosing crystals, leading to severe inflammation, and thus, expediting gout. These findings offer a potential explanation for why gout is preferred in OA joints and offer significant insights into the pathophysiology of gout, thereby informing prevention and management strategies for OA to prevent or alleviate the subsequent progression of gout. |
| format | Article |
| id | doaj-art-cc5c88d1336345239b1235853bb817b8 |
| institution | Kabale University |
| issn | 2688-2663 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
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| spelling | doaj-art-cc5c88d1336345239b1235853bb817b82025-01-20T01:45:44ZengWileyMedComm2688-26632025-01-0161n/an/a10.1002/mco2.70040Osteoarthritis synovium as a nidus for monosodium urate crystal deposition inducing severe gout studied by label‐free stimulated Raman scattering combined with synovial organoidsZiyi Chen0Wenjuan Wang1Yaxin Chen2Minbiao Ji3Yinghui Hua4Department of Sports Medicine Huashan Hospital Fudan University Shanghai ChinaDepartment of Sports Medicine Huashan Hospital Fudan University Shanghai ChinaState Key Laboratory of Surface Physics and Department of Physics Human Phenome Institute Multiscale Research Institute of Complex Systems Academy for Engineering and Technology Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education) Fudan University Shanghai ChinaState Key Laboratory of Surface Physics and Department of Physics Human Phenome Institute Multiscale Research Institute of Complex Systems Academy for Engineering and Technology Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education) Fudan University Shanghai ChinaDepartment of Sports Medicine Huashan Hospital Fudan University Shanghai ChinaAbstract Gout, a common chronic disease, is characterized by the formation and deposition of monosodium urate (MSU) crystal deposition in articular and nonarticular structures. Osteoarthritis (OA), the most prevalent type of arthritis, is a progressive degenerative joint disease. Previous clinical studies have reported that gout frequently affects OA joints; however, the underlying mechanism remains unidentified. Recently, OA synovium has been proposed as a favorable vehicle for MSU crystal deposition. Therefore, this study aimed to investigate whether OA synovium acts as a nidus for MSU crystal deposition inducing severe gout flares, using label‐free, highly‐specific stimulated Raman scattering (SRS) microscopy combined with innovative preclinical models—synovial organoids. Crystal deposition, cellular phagocytosis, and subsequent inflammation intensity was imaged in ex vivo synovial organoids using SRS microscopy and other biochemical techniques. Results revealed that MSU crystals were more likely to deposit in OA synovium than in normal synovium. Furthermore, OA synoviocytes were more capable of phagocytosing crystals, leading to severe inflammation, and thus, expediting gout. These findings offer a potential explanation for why gout is preferred in OA joints and offer significant insights into the pathophysiology of gout, thereby informing prevention and management strategies for OA to prevent or alleviate the subsequent progression of gout.https://doi.org/10.1002/mco2.70040goutmonosodium urate crystalsosteoarthritisstimulated Raman scatteringsynovial organoid |
| spellingShingle | Ziyi Chen Wenjuan Wang Yaxin Chen Minbiao Ji Yinghui Hua Osteoarthritis synovium as a nidus for monosodium urate crystal deposition inducing severe gout studied by label‐free stimulated Raman scattering combined with synovial organoids MedComm gout monosodium urate crystals osteoarthritis stimulated Raman scattering synovial organoid |
| title | Osteoarthritis synovium as a nidus for monosodium urate crystal deposition inducing severe gout studied by label‐free stimulated Raman scattering combined with synovial organoids |
| title_full | Osteoarthritis synovium as a nidus for monosodium urate crystal deposition inducing severe gout studied by label‐free stimulated Raman scattering combined with synovial organoids |
| title_fullStr | Osteoarthritis synovium as a nidus for monosodium urate crystal deposition inducing severe gout studied by label‐free stimulated Raman scattering combined with synovial organoids |
| title_full_unstemmed | Osteoarthritis synovium as a nidus for monosodium urate crystal deposition inducing severe gout studied by label‐free stimulated Raman scattering combined with synovial organoids |
| title_short | Osteoarthritis synovium as a nidus for monosodium urate crystal deposition inducing severe gout studied by label‐free stimulated Raman scattering combined with synovial organoids |
| title_sort | osteoarthritis synovium as a nidus for monosodium urate crystal deposition inducing severe gout studied by label free stimulated raman scattering combined with synovial organoids |
| topic | gout monosodium urate crystals osteoarthritis stimulated Raman scattering synovial organoid |
| url | https://doi.org/10.1002/mco2.70040 |
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