The generation of novel epialleles in plants: the prospective behind re-shaping the epigenome
Chromatin organization is a relevant layer of control of gene expression during plant development. Chromatin states strictly depend on associated features such as DNA methylation, histone modifications and histone variants. Thus, epigenome editing has become of primary interest to alter gene express...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1544744/full |
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| author | Alessio Baldini Filippo Battaglia Giorgio Perrella |
| author_facet | Alessio Baldini Filippo Battaglia Giorgio Perrella |
| author_sort | Alessio Baldini |
| collection | DOAJ |
| description | Chromatin organization is a relevant layer of control of gene expression during plant development. Chromatin states strictly depend on associated features such as DNA methylation, histone modifications and histone variants. Thus, epigenome editing has become of primary interest to alter gene expression without disrupting genomic sequences. Different tools have been developed to address this challenge, starting with modular Zinc Finger Proteins (ZFPs) and Transcription Activator Like Effectors (TALEs). However, the discovery of CRISPR/Cas9 system and the adaptability of technologies based on enzymatically dead Cas9 (dCas9) have paved the way towards a reliable and adaptable epigenome editing in a great variety of organisms. In this review, we will focus on the application of targeted epigenome editing technologies in plants, summarizing the most updated advances in this field. The promising results obtained by altering the expression state of targets involved in flowering time and abiotic stress resistance are crucial not only for elucidating the molecular interactions that underly chromatin dynamics, but also for future applications in breeding programs as an alternative route to genetic manipulation towards the achievement of higher quality crops particularly in terms of nutritional properties, yield and tolerance. |
| format | Article |
| id | doaj-art-cc43f028ee9f4579b6d34a74f40a1b01 |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-cc43f028ee9f4579b6d34a74f40a1b012025-08-20T01:49:14ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-03-011610.3389/fpls.2025.15447441544744The generation of novel epialleles in plants: the prospective behind re-shaping the epigenomeAlessio BaldiniFilippo BattagliaGiorgio PerrellaChromatin organization is a relevant layer of control of gene expression during plant development. Chromatin states strictly depend on associated features such as DNA methylation, histone modifications and histone variants. Thus, epigenome editing has become of primary interest to alter gene expression without disrupting genomic sequences. Different tools have been developed to address this challenge, starting with modular Zinc Finger Proteins (ZFPs) and Transcription Activator Like Effectors (TALEs). However, the discovery of CRISPR/Cas9 system and the adaptability of technologies based on enzymatically dead Cas9 (dCas9) have paved the way towards a reliable and adaptable epigenome editing in a great variety of organisms. In this review, we will focus on the application of targeted epigenome editing technologies in plants, summarizing the most updated advances in this field. The promising results obtained by altering the expression state of targets involved in flowering time and abiotic stress resistance are crucial not only for elucidating the molecular interactions that underly chromatin dynamics, but also for future applications in breeding programs as an alternative route to genetic manipulation towards the achievement of higher quality crops particularly in terms of nutritional properties, yield and tolerance.https://www.frontiersin.org/articles/10.3389/fpls.2025.1544744/fullgene editingCRISPR/Cas9zinc finger proteinsTALEsDNA methylationhistone modifications |
| spellingShingle | Alessio Baldini Filippo Battaglia Giorgio Perrella The generation of novel epialleles in plants: the prospective behind re-shaping the epigenome Frontiers in Plant Science gene editing CRISPR/Cas9 zinc finger proteins TALEs DNA methylation histone modifications |
| title | The generation of novel epialleles in plants: the prospective behind re-shaping the epigenome |
| title_full | The generation of novel epialleles in plants: the prospective behind re-shaping the epigenome |
| title_fullStr | The generation of novel epialleles in plants: the prospective behind re-shaping the epigenome |
| title_full_unstemmed | The generation of novel epialleles in plants: the prospective behind re-shaping the epigenome |
| title_short | The generation of novel epialleles in plants: the prospective behind re-shaping the epigenome |
| title_sort | generation of novel epialleles in plants the prospective behind re shaping the epigenome |
| topic | gene editing CRISPR/Cas9 zinc finger proteins TALEs DNA methylation histone modifications |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1544744/full |
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