The Relevance of CD117-Immunocytochemistry Staining Patterns to Mutational Exon-11 in c-kit Detected by PCR from Fine-Needle Aspirated Canine Mast Cell Tumor Cells
Canine cutaneous mast cell tumors (MCT) are the lethal skin tumors. The biological behavior of the MCT cells is quite varied and unpredictable. Almost MCT dogs usually require a rapid diagnosis and therapy. However, MCT diagnosis and prognosis are still dependent on histopathology which is rather in...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2014/787498 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832547532534710272 |
|---|---|
| author | A. Sailasuta D. Ketpun P. Piyaviriyakul S. Theerawatanasirikul P. Theewasutrakul A. Rungsipipat |
| author_facet | A. Sailasuta D. Ketpun P. Piyaviriyakul S. Theerawatanasirikul P. Theewasutrakul A. Rungsipipat |
| author_sort | A. Sailasuta |
| collection | DOAJ |
| description | Canine cutaneous mast cell tumors (MCT) are the lethal skin tumors. The biological behavior of the MCT cells is quite varied and unpredictable. Almost MCT dogs usually require a rapid diagnosis and therapy. However, MCT diagnosis and prognosis are still dependent on histopathology which is rather inconvenient, time-consuming, painful, and harmful for some cases. Indeed, MCT can be easily accessible using fine-needle aspiration (FNA). In this study, our biopsy specimens were classified as low- and high-grade MCT based on the novel 2-tier histopathologic grading system. We have demonstrated the usage of fine-needle aspirated MCT cells (FNA-MCT cells) from these specimens as a primary cell source to study the distribution of CD117-immunocytochemistry (CD117-ICC) staining patterns and the frequency of internal tandem duplication- (ITD-) mutant exon-11 of c-kit. The result has substantially shown that there were three staining patterns identified in the cells. Only paranuclear pattern was significantly increased in the cells from high-grade MCT. Altogether, the ITD-mutant exon-11 was also detectable only in these cells. Therefore, the result has supported our hypothesis that there was an increased opportunity to observe a higher CD117-ICC staining pattern and exon-11 mutation in high-grade MCT; even these two parameters may not precisely indicate a histopathological grade. |
| format | Article |
| id | doaj-art-cc335017b9054a0f8da65794d18c02c9 |
| institution | Kabale University |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-cc335017b9054a0f8da65794d18c02c92025-02-03T06:44:30ZengWileyVeterinary Medicine International2090-81132042-00482014-01-01201410.1155/2014/787498787498The Relevance of CD117-Immunocytochemistry Staining Patterns to Mutational Exon-11 in c-kit Detected by PCR from Fine-Needle Aspirated Canine Mast Cell Tumor CellsA. Sailasuta0D. Ketpun1P. Piyaviriyakul2S. Theerawatanasirikul3P. Theewasutrakul4A. Rungsipipat5STAR: Molecular Biology Research on Animal Oncology, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandSTAR: Molecular Biology Research on Animal Oncology, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandSTAR: Molecular Biology Research on Animal Oncology, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandSTAR: Molecular Biology Research on Animal Oncology, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandSTAR: Molecular Biology Research on Animal Oncology, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandSTAR: Molecular Biology Research on Animal Oncology, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandCanine cutaneous mast cell tumors (MCT) are the lethal skin tumors. The biological behavior of the MCT cells is quite varied and unpredictable. Almost MCT dogs usually require a rapid diagnosis and therapy. However, MCT diagnosis and prognosis are still dependent on histopathology which is rather inconvenient, time-consuming, painful, and harmful for some cases. Indeed, MCT can be easily accessible using fine-needle aspiration (FNA). In this study, our biopsy specimens were classified as low- and high-grade MCT based on the novel 2-tier histopathologic grading system. We have demonstrated the usage of fine-needle aspirated MCT cells (FNA-MCT cells) from these specimens as a primary cell source to study the distribution of CD117-immunocytochemistry (CD117-ICC) staining patterns and the frequency of internal tandem duplication- (ITD-) mutant exon-11 of c-kit. The result has substantially shown that there were three staining patterns identified in the cells. Only paranuclear pattern was significantly increased in the cells from high-grade MCT. Altogether, the ITD-mutant exon-11 was also detectable only in these cells. Therefore, the result has supported our hypothesis that there was an increased opportunity to observe a higher CD117-ICC staining pattern and exon-11 mutation in high-grade MCT; even these two parameters may not precisely indicate a histopathological grade.http://dx.doi.org/10.1155/2014/787498 |
| spellingShingle | A. Sailasuta D. Ketpun P. Piyaviriyakul S. Theerawatanasirikul P. Theewasutrakul A. Rungsipipat The Relevance of CD117-Immunocytochemistry Staining Patterns to Mutational Exon-11 in c-kit Detected by PCR from Fine-Needle Aspirated Canine Mast Cell Tumor Cells Veterinary Medicine International |
| title | The Relevance of CD117-Immunocytochemistry Staining Patterns to Mutational Exon-11 in c-kit Detected by PCR from Fine-Needle Aspirated Canine Mast Cell Tumor Cells |
| title_full | The Relevance of CD117-Immunocytochemistry Staining Patterns to Mutational Exon-11 in c-kit Detected by PCR from Fine-Needle Aspirated Canine Mast Cell Tumor Cells |
| title_fullStr | The Relevance of CD117-Immunocytochemistry Staining Patterns to Mutational Exon-11 in c-kit Detected by PCR from Fine-Needle Aspirated Canine Mast Cell Tumor Cells |
| title_full_unstemmed | The Relevance of CD117-Immunocytochemistry Staining Patterns to Mutational Exon-11 in c-kit Detected by PCR from Fine-Needle Aspirated Canine Mast Cell Tumor Cells |
| title_short | The Relevance of CD117-Immunocytochemistry Staining Patterns to Mutational Exon-11 in c-kit Detected by PCR from Fine-Needle Aspirated Canine Mast Cell Tumor Cells |
| title_sort | relevance of cd117 immunocytochemistry staining patterns to mutational exon 11 in c kit detected by pcr from fine needle aspirated canine mast cell tumor cells |
| url | http://dx.doi.org/10.1155/2014/787498 |
| work_keys_str_mv | AT asailasuta therelevanceofcd117immunocytochemistrystainingpatternstomutationalexon11inckitdetectedbypcrfromfineneedleaspiratedcaninemastcelltumorcells AT dketpun therelevanceofcd117immunocytochemistrystainingpatternstomutationalexon11inckitdetectedbypcrfromfineneedleaspiratedcaninemastcelltumorcells AT ppiyaviriyakul therelevanceofcd117immunocytochemistrystainingpatternstomutationalexon11inckitdetectedbypcrfromfineneedleaspiratedcaninemastcelltumorcells AT stheerawatanasirikul therelevanceofcd117immunocytochemistrystainingpatternstomutationalexon11inckitdetectedbypcrfromfineneedleaspiratedcaninemastcelltumorcells AT ptheewasutrakul therelevanceofcd117immunocytochemistrystainingpatternstomutationalexon11inckitdetectedbypcrfromfineneedleaspiratedcaninemastcelltumorcells AT arungsipipat therelevanceofcd117immunocytochemistrystainingpatternstomutationalexon11inckitdetectedbypcrfromfineneedleaspiratedcaninemastcelltumorcells AT asailasuta relevanceofcd117immunocytochemistrystainingpatternstomutationalexon11inckitdetectedbypcrfromfineneedleaspiratedcaninemastcelltumorcells AT dketpun relevanceofcd117immunocytochemistrystainingpatternstomutationalexon11inckitdetectedbypcrfromfineneedleaspiratedcaninemastcelltumorcells AT ppiyaviriyakul relevanceofcd117immunocytochemistrystainingpatternstomutationalexon11inckitdetectedbypcrfromfineneedleaspiratedcaninemastcelltumorcells AT stheerawatanasirikul relevanceofcd117immunocytochemistrystainingpatternstomutationalexon11inckitdetectedbypcrfromfineneedleaspiratedcaninemastcelltumorcells AT ptheewasutrakul relevanceofcd117immunocytochemistrystainingpatternstomutationalexon11inckitdetectedbypcrfromfineneedleaspiratedcaninemastcelltumorcells AT arungsipipat relevanceofcd117immunocytochemistrystainingpatternstomutationalexon11inckitdetectedbypcrfromfineneedleaspiratedcaninemastcelltumorcells |