PYR-41 and Thalidomide Impair Dendritic Cell Cross-Presentation by Inhibiting Myddosome Formation and Attenuating the Endosomal Recruitments of p97 and Sec61 via NF-κB Inactivation
PYR-41 and thalidomide have therapeutic effects on inflammation-associated diseases with side effects such as tumorigenesis. Cross-presentation allows dendritic cells (DC) to present endogenous antigen and induce protective immunity against microbe infection and tumors. But, up to now, the effects o...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/5070573 |
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| author | Xiang You Dan Dan Xu Di Zhang Jie Chen Feng Guang Gao |
| author_facet | Xiang You Dan Dan Xu Di Zhang Jie Chen Feng Guang Gao |
| author_sort | Xiang You |
| collection | DOAJ |
| description | PYR-41 and thalidomide have therapeutic effects on inflammation-associated diseases with side effects such as tumorigenesis. Cross-presentation allows dendritic cells (DC) to present endogenous antigen and induce protective immunity against microbe infection and tumors. But, up to now, the effects of PYR-41 and thalidomide on cross-presentation are still uncertain. In this study, we investigated the effect and mechanism of PYR-41 and thalidomide on DC cross-presentation by observing Myddosome formation, endosomal recruitment of p97 and Sec61, NF-κB activation, and cross-priming ability. We demonstrated that the inhibition of endosomal recruitment of p97 and Sec61, together with attenuated NF-κB activation and Myddosome formation, contributes to PYR-41- and thalidomide-impaired cross-presentation and thereby reverses cross-activation of T cells. These observations suggest that NF-κB signaling and p97 and Sec61 molecules are candidates for dealing with the side effects of PYR-41 and thalidomide. |
| format | Article |
| id | doaj-art-cc23cfbca3c5488398bffef5be047cbd |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-cc23cfbca3c5488398bffef5be047cbd2025-02-03T01:03:32ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/50705735070573PYR-41 and Thalidomide Impair Dendritic Cell Cross-Presentation by Inhibiting Myddosome Formation and Attenuating the Endosomal Recruitments of p97 and Sec61 via NF-κB InactivationXiang You0Dan Dan Xu1Di Zhang2Jie Chen3Feng Guang Gao4Department of Immunology, Basic Medicine Science, Medical College, Xiamen University, Xiamen, Fujian 361102, ChinaDepartment of Immunology, Basic Medicine Science, Medical College, Xiamen University, Xiamen, Fujian 361102, ChinaDepartment of Immunology, Basic Medicine Science, Medical College, Xiamen University, Xiamen, Fujian 361102, ChinaDepartment of Immunology, Basic Medicine Science, Medical College, Xiamen University, Xiamen, Fujian 361102, ChinaDepartment of Immunology, Basic Medicine Science, Medical College, Xiamen University, Xiamen, Fujian 361102, ChinaPYR-41 and thalidomide have therapeutic effects on inflammation-associated diseases with side effects such as tumorigenesis. Cross-presentation allows dendritic cells (DC) to present endogenous antigen and induce protective immunity against microbe infection and tumors. But, up to now, the effects of PYR-41 and thalidomide on cross-presentation are still uncertain. In this study, we investigated the effect and mechanism of PYR-41 and thalidomide on DC cross-presentation by observing Myddosome formation, endosomal recruitment of p97 and Sec61, NF-κB activation, and cross-priming ability. We demonstrated that the inhibition of endosomal recruitment of p97 and Sec61, together with attenuated NF-κB activation and Myddosome formation, contributes to PYR-41- and thalidomide-impaired cross-presentation and thereby reverses cross-activation of T cells. These observations suggest that NF-κB signaling and p97 and Sec61 molecules are candidates for dealing with the side effects of PYR-41 and thalidomide.http://dx.doi.org/10.1155/2018/5070573 |
| spellingShingle | Xiang You Dan Dan Xu Di Zhang Jie Chen Feng Guang Gao PYR-41 and Thalidomide Impair Dendritic Cell Cross-Presentation by Inhibiting Myddosome Formation and Attenuating the Endosomal Recruitments of p97 and Sec61 via NF-κB Inactivation Journal of Immunology Research |
| title | PYR-41 and Thalidomide Impair Dendritic Cell Cross-Presentation by Inhibiting Myddosome Formation and Attenuating the Endosomal Recruitments of p97 and Sec61 via NF-κB Inactivation |
| title_full | PYR-41 and Thalidomide Impair Dendritic Cell Cross-Presentation by Inhibiting Myddosome Formation and Attenuating the Endosomal Recruitments of p97 and Sec61 via NF-κB Inactivation |
| title_fullStr | PYR-41 and Thalidomide Impair Dendritic Cell Cross-Presentation by Inhibiting Myddosome Formation and Attenuating the Endosomal Recruitments of p97 and Sec61 via NF-κB Inactivation |
| title_full_unstemmed | PYR-41 and Thalidomide Impair Dendritic Cell Cross-Presentation by Inhibiting Myddosome Formation and Attenuating the Endosomal Recruitments of p97 and Sec61 via NF-κB Inactivation |
| title_short | PYR-41 and Thalidomide Impair Dendritic Cell Cross-Presentation by Inhibiting Myddosome Formation and Attenuating the Endosomal Recruitments of p97 and Sec61 via NF-κB Inactivation |
| title_sort | pyr 41 and thalidomide impair dendritic cell cross presentation by inhibiting myddosome formation and attenuating the endosomal recruitments of p97 and sec61 via nf κb inactivation |
| url | http://dx.doi.org/10.1155/2018/5070573 |
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