Induction of xenobiotic-metabolizing enzymes in hepatocytes by beta-naphthoflavone: Time-dependent changes in activities, protein and mRNA levels
In the present study, time-dependency of the induction effect of a selective inducer on the activity, protein and mRNA levels of cytochromes P450 1A1/2 (CYP1A1/2), NAD(P)H:quinone oxidoreductase 1 (NQO1) and glutathione S-transferases (GSTA), in primary culture of rat hepatocytes was tested and eval...
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| Format: | Article |
| Language: | English |
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Sciendo
2018-03-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2018-0005 |
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| author | Lněničková Kateřina Skálová Lenka Stuchlíková Lucie Szotáková Barbora Matoušková Petra |
| author_facet | Lněničková Kateřina Skálová Lenka Stuchlíková Lucie Szotáková Barbora Matoušková Petra |
| author_sort | Lněničková Kateřina |
| collection | DOAJ |
| description | In the present study, time-dependency of the induction effect of a selective inducer on the activity, protein and mRNA levels of cytochromes P450 1A1/2 (CYP1A1/2), NAD(P)H:quinone oxidoreductase 1 (NQO1) and glutathione S-transferases (GSTA), in primary culture of rat hepatocytes was tested and evaluated. To show the differences in responses of tested enzymes, the common aryl hydrocarbon receptor (AhR) ligand agonist, beta-naphthoflavone (BNF), was used. Induction of CYP1A1/2 by BNF was detected at all time intervals and at all levels (i.e., mRNA, protein, enzyme activity). Different responses of NQO1 and GSTA upon BNF treatment were observed. Our results demonstrate that the responses of different xenobiotic-metabolizing enzymes to the inducer vary in time and depend on the measured parameter. For these reasons, an induction study featuring only one-time interval treatment and/ or one parameter testing could produce misleading information. |
| format | Article |
| id | doaj-art-cbf3dc99f8284504ae45ec7a65668e97 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2018-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-cbf3dc99f8284504ae45ec7a65668e972025-02-02T11:49:57ZengSciendoActa Pharmaceutica1846-95582018-03-01681758510.2478/acph-2018-0005acph-2018-0005Induction of xenobiotic-metabolizing enzymes in hepatocytes by beta-naphthoflavone: Time-dependent changes in activities, protein and mRNA levelsLněničková Kateřina0Skálová Lenka1Stuchlíková Lucie2Szotáková Barbora3Matoušková Petra4Faculty of Pharmacy Charles University CZ-500 05 Hradec KrálovéCzech RepublicFaculty of Pharmacy Charles University CZ-500 05 Hradec KrálovéCzech RepublicFaculty of Pharmacy Charles University CZ-500 05 Hradec KrálovéCzech RepublicFaculty of Pharmacy Charles University CZ-500 05 Hradec KrálovéCzech RepublicFaculty of Pharmacy Charles University CZ-500 05 Hradec KrálovéCzech RepublicIn the present study, time-dependency of the induction effect of a selective inducer on the activity, protein and mRNA levels of cytochromes P450 1A1/2 (CYP1A1/2), NAD(P)H:quinone oxidoreductase 1 (NQO1) and glutathione S-transferases (GSTA), in primary culture of rat hepatocytes was tested and evaluated. To show the differences in responses of tested enzymes, the common aryl hydrocarbon receptor (AhR) ligand agonist, beta-naphthoflavone (BNF), was used. Induction of CYP1A1/2 by BNF was detected at all time intervals and at all levels (i.e., mRNA, protein, enzyme activity). Different responses of NQO1 and GSTA upon BNF treatment were observed. Our results demonstrate that the responses of different xenobiotic-metabolizing enzymes to the inducer vary in time and depend on the measured parameter. For these reasons, an induction study featuring only one-time interval treatment and/ or one parameter testing could produce misleading information.https://doi.org/10.2478/acph-2018-0005aryl hydrocarbon receptor (ahr)beta-naphthoflavonerat hepatocytetime dependencymrna-protein correlation |
| spellingShingle | Lněničková Kateřina Skálová Lenka Stuchlíková Lucie Szotáková Barbora Matoušková Petra Induction of xenobiotic-metabolizing enzymes in hepatocytes by beta-naphthoflavone: Time-dependent changes in activities, protein and mRNA levels Acta Pharmaceutica aryl hydrocarbon receptor (ahr) beta-naphthoflavone rat hepatocyte time dependency mrna-protein correlation |
| title | Induction of xenobiotic-metabolizing enzymes in hepatocytes by beta-naphthoflavone: Time-dependent changes in activities, protein and mRNA levels |
| title_full | Induction of xenobiotic-metabolizing enzymes in hepatocytes by beta-naphthoflavone: Time-dependent changes in activities, protein and mRNA levels |
| title_fullStr | Induction of xenobiotic-metabolizing enzymes in hepatocytes by beta-naphthoflavone: Time-dependent changes in activities, protein and mRNA levels |
| title_full_unstemmed | Induction of xenobiotic-metabolizing enzymes in hepatocytes by beta-naphthoflavone: Time-dependent changes in activities, protein and mRNA levels |
| title_short | Induction of xenobiotic-metabolizing enzymes in hepatocytes by beta-naphthoflavone: Time-dependent changes in activities, protein and mRNA levels |
| title_sort | induction of xenobiotic metabolizing enzymes in hepatocytes by beta naphthoflavone time dependent changes in activities protein and mrna levels |
| topic | aryl hydrocarbon receptor (ahr) beta-naphthoflavone rat hepatocyte time dependency mrna-protein correlation |
| url | https://doi.org/10.2478/acph-2018-0005 |
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