OPTIMIZATION OF DENATURATION TEMPERATURE AND TIME USING REAL-TIME PCR METHOD IN HEPATITIS B TEST
Background: The hepatitis B test can be conducted using the real-time PCR method. In chronic cases, the test is performed by detecting one of the Hepatitis B Virus (HBV) specific genes, HBcAg. The real-time PCR method requires optimization to obtain optimal results. Purpose: This research aims to de...
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Universitas Airlangga
2024-11-01
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| Series: | Journal of Vocational Health Studies |
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| Online Access: | https://e-journal.unair.ac.id/JVHS/article/view/47868 |
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| author | Tanti Yulia Dewi Fusvita Merdekawati Ai Djuminar Yogi Khoirul Abror |
| author_facet | Tanti Yulia Dewi Fusvita Merdekawati Ai Djuminar Yogi Khoirul Abror |
| author_sort | Tanti Yulia Dewi |
| collection | DOAJ |
| description | Background: The hepatitis B test can be conducted using the real-time PCR method. In chronic cases, the test is performed by detecting one of the Hepatitis B Virus (HBV) specific genes, HBcAg. The real-time PCR method requires optimization to obtain optimal results. Purpose: This research aims to determine the optimal temperature and time for denaturation in the hepatitis B test using the real-time PCR method. Method: The research method used was a quasi-experiment involving variations in temperature (94 °C, 95 °C, and 96 °C) and time (10 seconds, 15 seconds, and 20 seconds) for denaturation. Data processing resulted in static group comparisons based on 27 primary data from the Cycle Threshold (CT) value. Result: Variations in temperature conditions, specifically at 94 °C, 95 °C, and 96 °C, combined with a denaturation time of 10 seconds, yielded the mean CT values of 26.495, 26.355, and 26.003, respectively. When the temperature conditions were maintained at 94 °C, 95 °C, and 96 °C, with a denaturation time of 15 seconds, yielded the mean CT values of 25.962, 25.641, and 25.396. Similarly, under temperature conditions of 94 °C, 95 °C, and 96 °C with a denaturation time of 20 seconds, yielded the mean CT values of 26.544, 26.505, and 25.830 were obtained. Conclusion: Optimal results in this study are obtained through the acquisition of the smallest CT value, namely at temperature conditions of 96 °C and 15 seconds. |
| format | Article |
| id | doaj-art-cbf1dec318b24499a10f54306b6727f7 |
| institution | DOAJ |
| issn | 2580-7161 2580-717X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal of Vocational Health Studies |
| spelling | doaj-art-cbf1dec318b24499a10f54306b6727f72025-08-20T03:04:42ZengUniversitas AirlanggaJournal of Vocational Health Studies2580-71612580-717X2024-11-018213113610.20473/jvhs.V8.I2.2024.131-13645955OPTIMIZATION OF DENATURATION TEMPERATURE AND TIME USING REAL-TIME PCR METHOD IN HEPATITIS B TESTTanti Yulia Dewi0https://orcid.org/0009-0009-6009-0259Fusvita Merdekawati1https://orcid.org/0009-0000-9293-9610Ai Djuminar2https://orcid.org/0009-0007-4510-2333Yogi Khoirul Abror3https://orcid.org/0009-0002-9835-7595Department of Medical Laboratory Technology, Bandung Ministry of Health Polytechnic, BandungDepartment of Medical Laboratory Technology, Bandung Ministry of Health Polytechnic, BandungDepartment of Medical Laboratory Technology, Bandung Ministry of Health Polytechnic, BandungDepartment of Medical Laboratory Technology, Bandung Ministry of Health Polytechnic, BandungBackground: The hepatitis B test can be conducted using the real-time PCR method. In chronic cases, the test is performed by detecting one of the Hepatitis B Virus (HBV) specific genes, HBcAg. The real-time PCR method requires optimization to obtain optimal results. Purpose: This research aims to determine the optimal temperature and time for denaturation in the hepatitis B test using the real-time PCR method. Method: The research method used was a quasi-experiment involving variations in temperature (94 °C, 95 °C, and 96 °C) and time (10 seconds, 15 seconds, and 20 seconds) for denaturation. Data processing resulted in static group comparisons based on 27 primary data from the Cycle Threshold (CT) value. Result: Variations in temperature conditions, specifically at 94 °C, 95 °C, and 96 °C, combined with a denaturation time of 10 seconds, yielded the mean CT values of 26.495, 26.355, and 26.003, respectively. When the temperature conditions were maintained at 94 °C, 95 °C, and 96 °C, with a denaturation time of 15 seconds, yielded the mean CT values of 25.962, 25.641, and 25.396. Similarly, under temperature conditions of 94 °C, 95 °C, and 96 °C with a denaturation time of 20 seconds, yielded the mean CT values of 26.544, 26.505, and 25.830 were obtained. Conclusion: Optimal results in this study are obtained through the acquisition of the smallest CT value, namely at temperature conditions of 96 °C and 15 seconds.https://e-journal.unair.ac.id/JVHS/article/view/47868denaturationhbcag hepatitis bpcr real-time |
| spellingShingle | Tanti Yulia Dewi Fusvita Merdekawati Ai Djuminar Yogi Khoirul Abror OPTIMIZATION OF DENATURATION TEMPERATURE AND TIME USING REAL-TIME PCR METHOD IN HEPATITIS B TEST Journal of Vocational Health Studies denaturation hbcag hepatitis b pcr real-time |
| title | OPTIMIZATION OF DENATURATION TEMPERATURE AND TIME USING REAL-TIME PCR METHOD IN HEPATITIS B TEST |
| title_full | OPTIMIZATION OF DENATURATION TEMPERATURE AND TIME USING REAL-TIME PCR METHOD IN HEPATITIS B TEST |
| title_fullStr | OPTIMIZATION OF DENATURATION TEMPERATURE AND TIME USING REAL-TIME PCR METHOD IN HEPATITIS B TEST |
| title_full_unstemmed | OPTIMIZATION OF DENATURATION TEMPERATURE AND TIME USING REAL-TIME PCR METHOD IN HEPATITIS B TEST |
| title_short | OPTIMIZATION OF DENATURATION TEMPERATURE AND TIME USING REAL-TIME PCR METHOD IN HEPATITIS B TEST |
| title_sort | optimization of denaturation temperature and time using real time pcr method in hepatitis b test |
| topic | denaturation hbcag hepatitis b pcr real-time |
| url | https://e-journal.unair.ac.id/JVHS/article/view/47868 |
| work_keys_str_mv | AT tantiyuliadewi optimizationofdenaturationtemperatureandtimeusingrealtimepcrmethodinhepatitisbtest AT fusvitamerdekawati optimizationofdenaturationtemperatureandtimeusingrealtimepcrmethodinhepatitisbtest AT aidjuminar optimizationofdenaturationtemperatureandtimeusingrealtimepcrmethodinhepatitisbtest AT yogikhoirulabror optimizationofdenaturationtemperatureandtimeusingrealtimepcrmethodinhepatitisbtest |