Using grain protein electrophoresis to control the genetic integrity of spring bread wheat accessions from the VIR collection of plant genetic resources

Background. VIR's Department of Wheat holds the collections of viable accessions and its originals. The process of reproduction and restoration of germination ability in wheat collections requires monitoring of varietal purity and integrity. For this purpose, botanical varieties that describe s...

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Main Authors: E. V. Zuev, T. I. Peneva, N. M. Martynenko, A. N. Brykova, E. Yu. Kudryavtseva
Format: Article
Language:English
Published: N.I. Vavilov All-Russian Institute of Plant Genetic Resources 2019-01-01
Series:Труды по прикладной ботанике, генетике и селекции
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Online Access:https://elpub.vir.nw.ru/jour/article/view/298
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author E. V. Zuev
T. I. Peneva
N. M. Martynenko
A. N. Brykova
E. Yu. Kudryavtseva
author_facet E. V. Zuev
T. I. Peneva
N. M. Martynenko
A. N. Brykova
E. Yu. Kudryavtseva
author_sort E. V. Zuev
collection DOAJ
description Background. VIR's Department of Wheat holds the collections of viable accessions and its originals. The process of reproduction and restoration of germination ability in wheat collections requires monitoring of varietal purity and integrity. For this purpose, botanical varieties that describe sets of morphological characteristics of the spike and grain are used. However, sometimes it is necessary to check the conformity of a variety with its original accession. In this case, the gliadin electrophoretic banding patterns are reliable markers in identifying cultivars and biotypes of wheat.Materials and methods. The study included 7 accessions of spring bread wheat: 3 for the cultivar 'Rubin' and 4 for the cultivar 'Novinka'. Identification of accessions by electrophoresis of gliadin storage protein was carried out according to the generally accepted method.Results and conclusions. Comparison of gliadin electrophoretic banding patterns of the varieties from the VIR collection of plant genetic resources with analogous accessions from other genebanks of the world resulted in the recovery of the lost varieties 'Rubin' (Sweden) and 'Novinka' (Russia).
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institution Kabale University
issn 2227-8834
2619-0982
language English
publishDate 2019-01-01
publisher N.I. Vavilov All-Russian Institute of Plant Genetic Resources
record_format Article
series Труды по прикладной ботанике, генетике и селекции
spelling doaj-art-cbeec948642a43b08b7abe87a4091c982025-02-03T08:31:11ZengN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesТруды по прикладной ботанике, генетике и селекции2227-88342619-09822019-01-01179323523910.30901/2227-8834-2018-3-235-239296Using grain protein electrophoresis to control the genetic integrity of spring bread wheat accessions from the VIR collection of plant genetic resourcesE. V. Zuev0T. I. Peneva1N. M. Martynenko2A. N. Brykova3E. Yu. Kudryavtseva4N.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesBackground. VIR's Department of Wheat holds the collections of viable accessions and its originals. The process of reproduction and restoration of germination ability in wheat collections requires monitoring of varietal purity and integrity. For this purpose, botanical varieties that describe sets of morphological characteristics of the spike and grain are used. However, sometimes it is necessary to check the conformity of a variety with its original accession. In this case, the gliadin electrophoretic banding patterns are reliable markers in identifying cultivars and biotypes of wheat.Materials and methods. The study included 7 accessions of spring bread wheat: 3 for the cultivar 'Rubin' and 4 for the cultivar 'Novinka'. Identification of accessions by electrophoresis of gliadin storage protein was carried out according to the generally accepted method.Results and conclusions. Comparison of gliadin electrophoretic banding patterns of the varieties from the VIR collection of plant genetic resources with analogous accessions from other genebanks of the world resulted in the recovery of the lost varieties 'Rubin' (Sweden) and 'Novinka' (Russia).https://elpub.vir.nw.ru/jour/article/view/298spring bread wheatcultivargliadin electrophoretic banding pattern
spellingShingle E. V. Zuev
T. I. Peneva
N. M. Martynenko
A. N. Brykova
E. Yu. Kudryavtseva
Using grain protein electrophoresis to control the genetic integrity of spring bread wheat accessions from the VIR collection of plant genetic resources
Труды по прикладной ботанике, генетике и селекции
spring bread wheat
cultivar
gliadin electrophoretic banding pattern
title Using grain protein electrophoresis to control the genetic integrity of spring bread wheat accessions from the VIR collection of plant genetic resources
title_full Using grain protein electrophoresis to control the genetic integrity of spring bread wheat accessions from the VIR collection of plant genetic resources
title_fullStr Using grain protein electrophoresis to control the genetic integrity of spring bread wheat accessions from the VIR collection of plant genetic resources
title_full_unstemmed Using grain protein electrophoresis to control the genetic integrity of spring bread wheat accessions from the VIR collection of plant genetic resources
title_short Using grain protein electrophoresis to control the genetic integrity of spring bread wheat accessions from the VIR collection of plant genetic resources
title_sort using grain protein electrophoresis to control the genetic integrity of spring bread wheat accessions from the vir collection of plant genetic resources
topic spring bread wheat
cultivar
gliadin electrophoretic banding pattern
url https://elpub.vir.nw.ru/jour/article/view/298
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