A 3D Porous Gelatin-Alginate-Based-IPN Acts as an Efficient Promoter of Chondrogenesis from Human Adipose-Derived Stem Cells
Cartilage has limited regeneration potential. Thus, there is an imperative need to develop new strategies for cartilage tissue engineering (CTE) amenable for clinical use. Recent CTE approaches rely on optimal cell-scaffold interactions, which require a great deal of optimization. In this study we a...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/252909 |
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| author | Sorina Dinescu Bianca Galateanu Eugen Radu Anca Hermenean Adriana Lungu Izabela Cristina Stancu Dana Jianu Tudorita Tumbar Marieta Costache |
| author_facet | Sorina Dinescu Bianca Galateanu Eugen Radu Anca Hermenean Adriana Lungu Izabela Cristina Stancu Dana Jianu Tudorita Tumbar Marieta Costache |
| author_sort | Sorina Dinescu |
| collection | DOAJ |
| description | Cartilage has limited regeneration potential. Thus, there is an imperative need to develop new strategies for cartilage tissue engineering (CTE) amenable for clinical use. Recent CTE approaches rely on optimal cell-scaffold interactions, which require a great deal of optimization. In this study we attempt to build a novel gelatin- (G-) alginate- (A-) polyacrylamide (PAA) 3D interpenetrating network (IPN) with superior performance in promoting chondrogenesis from human adipose-derived stem cells (hADSCs). We show that our G-A-PAA scaffold is capable of supporting hADSCs proliferation and survival, with no apparent cytotoxic effect. Moreover, we find that after exposure to prochondrogenic conditions a key transcription factor known to induce chondrogenesis, namely, Sox9, is highly expressed in our hADSCs/G-A-PAA bioconstruct, along with cartilage specific markers such as collagen type II, CEP68, and COMP extracellular matrix (ECM) components. These data suggest that our G-A-PAA structural properties and formulation might enable hADSCs conversion towards functional chondrocytes. We conclude that our novel G-A-PAA biomatrix is a good candidate for prospective in vivo CTE applications. |
| format | Article |
| id | doaj-art-cbeb8590632248009d6f97811086b711 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-cbeb8590632248009d6f97811086b7112025-02-03T05:58:47ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/252909252909A 3D Porous Gelatin-Alginate-Based-IPN Acts as an Efficient Promoter of Chondrogenesis from Human Adipose-Derived Stem CellsSorina Dinescu0Bianca Galateanu1Eugen Radu2Anca Hermenean3Adriana Lungu4Izabela Cristina Stancu5Dana Jianu6Tudorita Tumbar7Marieta Costache8Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, RomaniaDepartment of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, RomaniaMolecular Biology and Pathology Research Lab “Molimagex”, University Hospital Bucharest, 169 Splaiul Independentei, 050098 Bucharest, RomaniaInstitute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, 310414 Arad, RomaniaAdvanced Polymer Materials Group, University Politehnica of Bucharest, 1-7 Gheorghe Polizu Street, District 1, 011061 Bucharest, RomaniaAdvanced Polymer Materials Group, University Politehnica of Bucharest, 1-7 Gheorghe Polizu Street, District 1, 011061 Bucharest, RomaniaProestetica Medical S.R.L., 38-40 T. Stefan Street, 011658 Bucharest, RomaniaDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USADepartment of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, RomaniaCartilage has limited regeneration potential. Thus, there is an imperative need to develop new strategies for cartilage tissue engineering (CTE) amenable for clinical use. Recent CTE approaches rely on optimal cell-scaffold interactions, which require a great deal of optimization. In this study we attempt to build a novel gelatin- (G-) alginate- (A-) polyacrylamide (PAA) 3D interpenetrating network (IPN) with superior performance in promoting chondrogenesis from human adipose-derived stem cells (hADSCs). We show that our G-A-PAA scaffold is capable of supporting hADSCs proliferation and survival, with no apparent cytotoxic effect. Moreover, we find that after exposure to prochondrogenic conditions a key transcription factor known to induce chondrogenesis, namely, Sox9, is highly expressed in our hADSCs/G-A-PAA bioconstruct, along with cartilage specific markers such as collagen type II, CEP68, and COMP extracellular matrix (ECM) components. These data suggest that our G-A-PAA structural properties and formulation might enable hADSCs conversion towards functional chondrocytes. We conclude that our novel G-A-PAA biomatrix is a good candidate for prospective in vivo CTE applications.http://dx.doi.org/10.1155/2015/252909 |
| spellingShingle | Sorina Dinescu Bianca Galateanu Eugen Radu Anca Hermenean Adriana Lungu Izabela Cristina Stancu Dana Jianu Tudorita Tumbar Marieta Costache A 3D Porous Gelatin-Alginate-Based-IPN Acts as an Efficient Promoter of Chondrogenesis from Human Adipose-Derived Stem Cells Stem Cells International |
| title | A 3D Porous Gelatin-Alginate-Based-IPN Acts as an Efficient Promoter of Chondrogenesis from Human Adipose-Derived Stem Cells |
| title_full | A 3D Porous Gelatin-Alginate-Based-IPN Acts as an Efficient Promoter of Chondrogenesis from Human Adipose-Derived Stem Cells |
| title_fullStr | A 3D Porous Gelatin-Alginate-Based-IPN Acts as an Efficient Promoter of Chondrogenesis from Human Adipose-Derived Stem Cells |
| title_full_unstemmed | A 3D Porous Gelatin-Alginate-Based-IPN Acts as an Efficient Promoter of Chondrogenesis from Human Adipose-Derived Stem Cells |
| title_short | A 3D Porous Gelatin-Alginate-Based-IPN Acts as an Efficient Promoter of Chondrogenesis from Human Adipose-Derived Stem Cells |
| title_sort | 3d porous gelatin alginate based ipn acts as an efficient promoter of chondrogenesis from human adipose derived stem cells |
| url | http://dx.doi.org/10.1155/2015/252909 |
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