Upregulation of p53 by tannic acid treatment suppresses the proliferation of human colorectal carcinoma
The present study’s objective is to clarify the molecular mechanisms of tannic acid effects on the viability of human colorectal carcinoma (CRC). Tannic acid is stable for up to 48 h and is localized in both cytoplasm and nucleus. It dose-dependently inhibited the viability of CRC cell lines; SW-620...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2021-12-01
|
| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acph-2021-0036 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832570528032882688 |
|---|---|
| author | Karakurt Serdar Kandir Sinan Gökçek-Saraç Çiğdem |
| author_facet | Karakurt Serdar Kandir Sinan Gökçek-Saraç Çiğdem |
| author_sort | Karakurt Serdar |
| collection | DOAJ |
| description | The present study’s objective is to clarify the molecular mechanisms of tannic acid effects on the viability of human colorectal carcinoma (CRC). Tannic acid is stable for up to 48 h and is localized in both cytoplasm and nucleus. It dose-dependently inhibited the viability of CRC cell lines; SW-620 and HT-29 with IC50 values of 7.2 ± 0.8 and 37.6 ± 1.4 µmol L–1. Besides, metastatic, invasive, and colony formation properties of CRC cells were significantly inhibited following the tannic acid treatment (p < 0.001). Tannic acid has been found to modulate enzyme, protein, and gene expressions of NQO1 in different levels and the upregulation of protein/gene expressions of p53 (p < 0.001), which leads the cells to trigger apoptosis. In conclusion, the present in vitro study may supply a significant background for in vivo studies in which the molecular mechanisms of antioxidant and chemopreventive activities of tannic acid will completely clarify. |
| format | Article |
| id | doaj-art-cbab129d84b3455ebd0ffee3c3460a7f |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-cbab129d84b3455ebd0ffee3c3460a7f2025-02-02T15:22:06ZengSciendoActa Pharmaceutica1846-95582021-12-0171458760210.2478/acph-2021-0036Upregulation of p53 by tannic acid treatment suppresses the proliferation of human colorectal carcinomaKarakurt Serdar0Kandir Sinan1Gökçek-Saraç Çiğdem2Selcuk University, Faculty of Science, Department of Biochemistry, KonyaTurkeyCukurova University, Ceyhan Faculty of Veterinary Medicine, Department of Physiology, Adana, TurkeyFaculty of Engineering, Department of Biomedical Engineering, Akdeniz University, Antalya, TurkeyThe present study’s objective is to clarify the molecular mechanisms of tannic acid effects on the viability of human colorectal carcinoma (CRC). Tannic acid is stable for up to 48 h and is localized in both cytoplasm and nucleus. It dose-dependently inhibited the viability of CRC cell lines; SW-620 and HT-29 with IC50 values of 7.2 ± 0.8 and 37.6 ± 1.4 µmol L–1. Besides, metastatic, invasive, and colony formation properties of CRC cells were significantly inhibited following the tannic acid treatment (p < 0.001). Tannic acid has been found to modulate enzyme, protein, and gene expressions of NQO1 in different levels and the upregulation of protein/gene expressions of p53 (p < 0.001), which leads the cells to trigger apoptosis. In conclusion, the present in vitro study may supply a significant background for in vivo studies in which the molecular mechanisms of antioxidant and chemopreventive activities of tannic acid will completely clarify.https://doi.org/10.2478/acph-2021-0036tannic acidcolorectal carcinomawound healingcell viabilitynqo1p53 |
| spellingShingle | Karakurt Serdar Kandir Sinan Gökçek-Saraç Çiğdem Upregulation of p53 by tannic acid treatment suppresses the proliferation of human colorectal carcinoma Acta Pharmaceutica tannic acid colorectal carcinoma wound healing cell viability nqo1 p53 |
| title | Upregulation of p53 by tannic acid treatment suppresses the proliferation of human colorectal carcinoma |
| title_full | Upregulation of p53 by tannic acid treatment suppresses the proliferation of human colorectal carcinoma |
| title_fullStr | Upregulation of p53 by tannic acid treatment suppresses the proliferation of human colorectal carcinoma |
| title_full_unstemmed | Upregulation of p53 by tannic acid treatment suppresses the proliferation of human colorectal carcinoma |
| title_short | Upregulation of p53 by tannic acid treatment suppresses the proliferation of human colorectal carcinoma |
| title_sort | upregulation of p53 by tannic acid treatment suppresses the proliferation of human colorectal carcinoma |
| topic | tannic acid colorectal carcinoma wound healing cell viability nqo1 p53 |
| url | https://doi.org/10.2478/acph-2021-0036 |
| work_keys_str_mv | AT karakurtserdar upregulationofp53bytannicacidtreatmentsuppressestheproliferationofhumancolorectalcarcinoma AT kandirsinan upregulationofp53bytannicacidtreatmentsuppressestheproliferationofhumancolorectalcarcinoma AT gokceksaraccigdem upregulationofp53bytannicacidtreatmentsuppressestheproliferationofhumancolorectalcarcinoma |