Acute and Subchronic Toxicity Studies on the Aqueous Extract of the Plant Mixture (Bidens pilosa and Cymbopogon citratus Aerial Parts) in Rat Model
Bidens pilosa (B. pilosa) and Cymbopogon citratus (C. citratus) are plants used individually or in combination in the traditional treatment of several ailments such as cardiovascular disorders. In order to valorise their traditional use, a toxicological study was conducted on the aqueous extract of...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2022/1998433 |
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| author | Yannick Carlos Tcheutchoua Danielle Claude Bilanda Yolande Sandrine Mengue Ngadena Paul Désiré Djomeni Dzeufiet Pascal Emmanuel Owona Ronald Bidingha á Goufani Rodrigue Ngapout Fifen Lohik Mbolang Nguegan Michel Noubom Théophile Dimo Pierre Kamtchouing |
| author_facet | Yannick Carlos Tcheutchoua Danielle Claude Bilanda Yolande Sandrine Mengue Ngadena Paul Désiré Djomeni Dzeufiet Pascal Emmanuel Owona Ronald Bidingha á Goufani Rodrigue Ngapout Fifen Lohik Mbolang Nguegan Michel Noubom Théophile Dimo Pierre Kamtchouing |
| author_sort | Yannick Carlos Tcheutchoua |
| collection | DOAJ |
| description | Bidens pilosa (B. pilosa) and Cymbopogon citratus (C. citratus) are plants used individually or in combination in the traditional treatment of several ailments such as cardiovascular disorders. In order to valorise their traditional use, a toxicological study was conducted on the aqueous extract of the mixture of aerial parts of B. pilosa and C. citratus. The acute and subchronic toxicity studies were conducted according to the OECD 425 and 407 guidelines. Regarding the acute study, the aqueous extract of the mixture of B. pilosa and C. citratus 50 : 50 (2000 and 5000 mg/kg) was administered once to rats of both sexes. In the subchronic study, the aqueous extract of the mixture of B. pilosa and C. citratus (200, 400 and 800 mg/kg) was administered once daily to rats for 28 days. The aqueous extract of the mixture of B. pilosa and C. citratus (2000 and 5000 mg/kg) did not cause death and did not induce any apparent sign of toxicity during the 14 days of observation. The DL50 of the extract is therefore greater than 5000 mg/kg. Taken daily for 28 days, the extract had no significant effect on selected parameters (creatinine, AST, ALT, urea, and uric acid) of renal and hepatic function, as well as on the number of some blood cells. However, the aqueous extract of the mixture of B. pilosa and C. citratus (200 and 400 mg/kg) caused a significant (p < 0.05; p < 0.001, respectively) decrease in creatinine levels in male rats as compared to normal control animals. In females, the aqueous extract of the mixture of B. pilosa and C. citratus (200 and 400 mg/kg) resulted in a significant (p < 0.05) increase in total cholesterol levels as compared to normal control animals. The study showed that the aqueous extract of the mixture of B. pilosa and C. citratus has a low toxicity and does not cause any injury to the liver, kidney, lungs, or spleen. |
| format | Article |
| id | doaj-art-cb9b6e0a3b1f4b7c917da4063889401a |
| institution | Kabale University |
| issn | 1687-8205 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-cb9b6e0a3b1f4b7c917da4063889401a2025-02-03T01:00:44ZengWileyJournal of Toxicology1687-82052022-01-01202210.1155/2022/1998433Acute and Subchronic Toxicity Studies on the Aqueous Extract of the Plant Mixture (Bidens pilosa and Cymbopogon citratus Aerial Parts) in Rat ModelYannick Carlos Tcheutchoua0Danielle Claude Bilanda1Yolande Sandrine Mengue Ngadena2Paul Désiré Djomeni Dzeufiet3Pascal Emmanuel Owona4Ronald Bidingha á Goufani5Rodrigue Ngapout Fifen6Lohik Mbolang Nguegan7Michel Noubom8Théophile Dimo9Pierre Kamtchouing10Department of Animal Biology and PhysiologyDepartment of Animal Biology and PhysiologyDepartment of PsychologyDepartment of Animal Biology and PhysiologyDepartment of Animal Biology and PhysiologyDepartment of Animal Biology and PhysiologyDepartment of Animal Biology and PhysiologyDepartment of Animal Biology and PhysiologyDepartment of Biological SciencesDepartment of Animal Biology and PhysiologyDepartment of Animal Biology and PhysiologyBidens pilosa (B. pilosa) and Cymbopogon citratus (C. citratus) are plants used individually or in combination in the traditional treatment of several ailments such as cardiovascular disorders. In order to valorise their traditional use, a toxicological study was conducted on the aqueous extract of the mixture of aerial parts of B. pilosa and C. citratus. The acute and subchronic toxicity studies were conducted according to the OECD 425 and 407 guidelines. Regarding the acute study, the aqueous extract of the mixture of B. pilosa and C. citratus 50 : 50 (2000 and 5000 mg/kg) was administered once to rats of both sexes. In the subchronic study, the aqueous extract of the mixture of B. pilosa and C. citratus (200, 400 and 800 mg/kg) was administered once daily to rats for 28 days. The aqueous extract of the mixture of B. pilosa and C. citratus (2000 and 5000 mg/kg) did not cause death and did not induce any apparent sign of toxicity during the 14 days of observation. The DL50 of the extract is therefore greater than 5000 mg/kg. Taken daily for 28 days, the extract had no significant effect on selected parameters (creatinine, AST, ALT, urea, and uric acid) of renal and hepatic function, as well as on the number of some blood cells. However, the aqueous extract of the mixture of B. pilosa and C. citratus (200 and 400 mg/kg) caused a significant (p < 0.05; p < 0.001, respectively) decrease in creatinine levels in male rats as compared to normal control animals. In females, the aqueous extract of the mixture of B. pilosa and C. citratus (200 and 400 mg/kg) resulted in a significant (p < 0.05) increase in total cholesterol levels as compared to normal control animals. The study showed that the aqueous extract of the mixture of B. pilosa and C. citratus has a low toxicity and does not cause any injury to the liver, kidney, lungs, or spleen.http://dx.doi.org/10.1155/2022/1998433 |
| spellingShingle | Yannick Carlos Tcheutchoua Danielle Claude Bilanda Yolande Sandrine Mengue Ngadena Paul Désiré Djomeni Dzeufiet Pascal Emmanuel Owona Ronald Bidingha á Goufani Rodrigue Ngapout Fifen Lohik Mbolang Nguegan Michel Noubom Théophile Dimo Pierre Kamtchouing Acute and Subchronic Toxicity Studies on the Aqueous Extract of the Plant Mixture (Bidens pilosa and Cymbopogon citratus Aerial Parts) in Rat Model Journal of Toxicology |
| title | Acute and Subchronic Toxicity Studies on the Aqueous Extract of the Plant Mixture (Bidens pilosa and Cymbopogon citratus Aerial Parts) in Rat Model |
| title_full | Acute and Subchronic Toxicity Studies on the Aqueous Extract of the Plant Mixture (Bidens pilosa and Cymbopogon citratus Aerial Parts) in Rat Model |
| title_fullStr | Acute and Subchronic Toxicity Studies on the Aqueous Extract of the Plant Mixture (Bidens pilosa and Cymbopogon citratus Aerial Parts) in Rat Model |
| title_full_unstemmed | Acute and Subchronic Toxicity Studies on the Aqueous Extract of the Plant Mixture (Bidens pilosa and Cymbopogon citratus Aerial Parts) in Rat Model |
| title_short | Acute and Subchronic Toxicity Studies on the Aqueous Extract of the Plant Mixture (Bidens pilosa and Cymbopogon citratus Aerial Parts) in Rat Model |
| title_sort | acute and subchronic toxicity studies on the aqueous extract of the plant mixture bidens pilosa and cymbopogon citratus aerial parts in rat model |
| url | http://dx.doi.org/10.1155/2022/1998433 |
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