Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology
The aim of the present study was to determine the antioxidant activity of Coreopsis tinctoria flowering tops (CTFT). Studies were conducted to obtain suitable extraction conditions for chlorogenic acid, quercetin, luteolin, apigenin and kaempferol, which were identified and quantified by HPLC. Respo...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2014-09-01
|
| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acph-2014-0026 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832572179336658944 |
|---|---|
| author | Lan Shumin Lin Jingming Zheng Ni |
| author_facet | Lan Shumin Lin Jingming Zheng Ni |
| author_sort | Lan Shumin |
| collection | DOAJ |
| description | The aim of the present study was to determine the antioxidant activity of Coreopsis tinctoria flowering tops (CTFT). Studies were conducted to obtain suitable extraction conditions for chlorogenic acid, quercetin, luteolin, apigenin and kaempferol, which were identified and quantified by HPLC. Response surface methodology was employed to optimise the ultrasound-assisted extraction conditions including extraction time, ethanol concentration and liquid-solid ratio. The antioxidant activity of the extracts was analysed using various antioxidant models, such as DPPH, ABTS and hydroxyl radical scavenging assay. CTFT extracted for 15.0 min with ethanol at a concentration of 60.4 % and with liquidsolid ratio 27.5:1 possessed a considerable amounts of total flavonoids and polyphenols (18.9 %). This extract showed higher scavenging activity of ABTS and hydroxyl radical activity than rutin, however not in the DPPH test. We may assume that CTFT possess antioxidant and free radical scavenging potentials. |
| format | Article |
| id | doaj-art-cb91b7a33dc64f10a484e0b453afcb6c |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2014-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-cb91b7a33dc64f10a484e0b453afcb6c2025-02-02T11:33:21ZengSciendoActa Pharmaceutica1846-95582014-09-0164336937810.2478/acph-2014-0026acph-2014-0026Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface MethodologyLan Shumin0Lin Jingming1Zheng Ni2Department of Pharmacy, Guangdong No. 2 Provincial People’s Hospital Guangzhou 510317, PR ChinaDepartment of Pharmacy, Zhujiang Hospital, Southern Medical University Guangzhou 510282, PR ChinaDepartment of Pharmacy, Guangdong No. 2 Provincial People’s Hospital Guangzhou 510317, PR ChinaThe aim of the present study was to determine the antioxidant activity of Coreopsis tinctoria flowering tops (CTFT). Studies were conducted to obtain suitable extraction conditions for chlorogenic acid, quercetin, luteolin, apigenin and kaempferol, which were identified and quantified by HPLC. Response surface methodology was employed to optimise the ultrasound-assisted extraction conditions including extraction time, ethanol concentration and liquid-solid ratio. The antioxidant activity of the extracts was analysed using various antioxidant models, such as DPPH, ABTS and hydroxyl radical scavenging assay. CTFT extracted for 15.0 min with ethanol at a concentration of 60.4 % and with liquidsolid ratio 27.5:1 possessed a considerable amounts of total flavonoids and polyphenols (18.9 %). This extract showed higher scavenging activity of ABTS and hydroxyl radical activity than rutin, however not in the DPPH test. We may assume that CTFT possess antioxidant and free radical scavenging potentials.https://doi.org/10.2478/acph-2014-0026coreopsis tinctoria (asteraceae)flavonoidspolyphenolsantioxidant activityresponse surface methodologyhplc |
| spellingShingle | Lan Shumin Lin Jingming Zheng Ni Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology Acta Pharmaceutica coreopsis tinctoria (asteraceae) flavonoids polyphenols antioxidant activity response surface methodology hplc |
| title | Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology |
| title_full | Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology |
| title_fullStr | Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology |
| title_full_unstemmed | Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology |
| title_short | Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology |
| title_sort | evaluation of the antioxidant activity of coreopsis tinctoria nuff and optimisation of isolation by response surface methodology |
| topic | coreopsis tinctoria (asteraceae) flavonoids polyphenols antioxidant activity response surface methodology hplc |
| url | https://doi.org/10.2478/acph-2014-0026 |
| work_keys_str_mv | AT lanshumin evaluationoftheantioxidantactivityofcoreopsistinctorianuffandoptimisationofisolationbyresponsesurfacemethodology AT linjingming evaluationoftheantioxidantactivityofcoreopsistinctorianuffandoptimisationofisolationbyresponsesurfacemethodology AT zhengni evaluationoftheantioxidantactivityofcoreopsistinctorianuffandoptimisationofisolationbyresponsesurfacemethodology |