A novel dual fixation method for improving the reliable assessment of pulmonary vascular morphology in pulmonary hypertension rats
Abstract This study introduced a novel dual fixation method for the pulmonary vasculature and lung tissue in pulmonary hypertension (PH) rats, addressing the limitations of traditional fixation methods that failed to accurately preserve the in vivo status of pulmonary vascular morphology. The modifi...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | Respiratory Research |
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| Online Access: | https://doi.org/10.1186/s12931-024-03091-8 |
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| author | Yan Xu Pu Liao Xinyu Song Wenchuan Guo Bingxun Liu Tong Ye Ting Zhang Rui Xiao Liping Zhu Yujun Shen Yanjiang Xing Jing Wang Qinghua Hu |
| author_facet | Yan Xu Pu Liao Xinyu Song Wenchuan Guo Bingxun Liu Tong Ye Ting Zhang Rui Xiao Liping Zhu Yujun Shen Yanjiang Xing Jing Wang Qinghua Hu |
| author_sort | Yan Xu |
| collection | DOAJ |
| description | Abstract This study introduced a novel dual fixation method for the pulmonary vasculature and lung tissue in pulmonary hypertension (PH) rats, addressing the limitations of traditional fixation methods that failed to accurately preserve the in vivo status of pulmonary vascular morphology. The modified method involved a dual fixation process, combining individualized ventilation support and vascular perfusion to simulate the respiratory motion, pulmonary artery pressure and right ventricular output of the rat under in vivo conditions. Utilizing a monocrotaline-induced PH rat model, this study compared the dual fixation with the traditional immersion fixation, focusing on the quantitative assessment of alveolar expansion degree, capillary patency, endothelial cell quantity and wall thickness of pulmonary vein and artery. The results demonstrated that the dual fixation is superior in maintaining the authenticity and integrity of lung tissue and more sensitive in the evaluation of pulmonary artery hypertrophy, providing a more reliable representation of pulmonary vascular remodeling associated with PH. |
| format | Article |
| id | doaj-art-cb77fa694ddf4d29a0fe69d2af53c202 |
| institution | Kabale University |
| issn | 1465-993X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj-art-cb77fa694ddf4d29a0fe69d2af53c2022025-01-19T12:36:27ZengBMCRespiratory Research1465-993X2025-01-0126111210.1186/s12931-024-03091-8A novel dual fixation method for improving the reliable assessment of pulmonary vascular morphology in pulmonary hypertension ratsYan Xu0Pu Liao1Xinyu Song2Wenchuan Guo3Bingxun Liu4Tong Ye5Ting Zhang6Rui Xiao7Liping Zhu8Yujun Shen9Yanjiang Xing10Jing Wang11Qinghua Hu12Department of Pathophysiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology (HUST)Department of Pathology, Union Hospital, Tongji Medical College, HUSTDepartment of Respiratory and Critical Care Medicine, Affiliated Yichang Central People’s Hospital of China Three Gorges UniversityDepartment of Pathophysiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology (HUST)Department of Pathophysiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology (HUST)Department of Pathophysiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology (HUST)Key Laboratory of Pulmonary Diseases of Ministry of Health, Tongji Medical College, HUSTDepartment of Pathophysiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology (HUST)Department of Pathophysiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology (HUST)Department of Pharmacology, Tianjing Key Laboratory of Inflammatory Biology, School of Basic Medical Sciences, Tianjing Medical UniversityState Key Laboratory of Respiratory Health and Multimorbidity, Institute of Basic Medical Sciences, School of Basic Medicine, Chinese Academy of Medical Sciences, Peking Union Medical CollegeState Key Laboratory of Respiratory Health and Multimorbidity, Institute of Basic Medical Sciences, School of Basic Medicine, Chinese Academy of Medical Sciences, Peking Union Medical CollegeDepartment of Pathophysiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology (HUST)Abstract This study introduced a novel dual fixation method for the pulmonary vasculature and lung tissue in pulmonary hypertension (PH) rats, addressing the limitations of traditional fixation methods that failed to accurately preserve the in vivo status of pulmonary vascular morphology. The modified method involved a dual fixation process, combining individualized ventilation support and vascular perfusion to simulate the respiratory motion, pulmonary artery pressure and right ventricular output of the rat under in vivo conditions. Utilizing a monocrotaline-induced PH rat model, this study compared the dual fixation with the traditional immersion fixation, focusing on the quantitative assessment of alveolar expansion degree, capillary patency, endothelial cell quantity and wall thickness of pulmonary vein and artery. The results demonstrated that the dual fixation is superior in maintaining the authenticity and integrity of lung tissue and more sensitive in the evaluation of pulmonary artery hypertrophy, providing a more reliable representation of pulmonary vascular remodeling associated with PH.https://doi.org/10.1186/s12931-024-03091-8Lung tissue fixationPulmonary hypertensionPulmonary vascular morphology |
| spellingShingle | Yan Xu Pu Liao Xinyu Song Wenchuan Guo Bingxun Liu Tong Ye Ting Zhang Rui Xiao Liping Zhu Yujun Shen Yanjiang Xing Jing Wang Qinghua Hu A novel dual fixation method for improving the reliable assessment of pulmonary vascular morphology in pulmonary hypertension rats Respiratory Research Lung tissue fixation Pulmonary hypertension Pulmonary vascular morphology |
| title | A novel dual fixation method for improving the reliable assessment of pulmonary vascular morphology in pulmonary hypertension rats |
| title_full | A novel dual fixation method for improving the reliable assessment of pulmonary vascular morphology in pulmonary hypertension rats |
| title_fullStr | A novel dual fixation method for improving the reliable assessment of pulmonary vascular morphology in pulmonary hypertension rats |
| title_full_unstemmed | A novel dual fixation method for improving the reliable assessment of pulmonary vascular morphology in pulmonary hypertension rats |
| title_short | A novel dual fixation method for improving the reliable assessment of pulmonary vascular morphology in pulmonary hypertension rats |
| title_sort | novel dual fixation method for improving the reliable assessment of pulmonary vascular morphology in pulmonary hypertension rats |
| topic | Lung tissue fixation Pulmonary hypertension Pulmonary vascular morphology |
| url | https://doi.org/10.1186/s12931-024-03091-8 |
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