Evaluation of wound healing activity of the crude extract and solvent fractions of Vahl (Polygonaceae) leaves in mice
Background In Ethiopian traditional medicine, Rumex nervosus Vahl leaves are used to treat wounds. However, despite traditional claims and supportive in vitro findings, no scientific study has been conducted to evaluate the in vivo wound healing activity of R. nervosus Vahl leaves. Aim of the study...
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SAGE Publishing
2025-01-01
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| Series: | Scars, Burns & Healing |
| Online Access: | https://doi.org/10.1177/20595131251316791 |
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| author | Abel Andualem Kefyalew Ayalew Getahun Bahiru Tenaw Goshu Yaschilal Muche Belayneh |
| author_facet | Abel Andualem Kefyalew Ayalew Getahun Bahiru Tenaw Goshu Yaschilal Muche Belayneh |
| author_sort | Abel Andualem |
| collection | DOAJ |
| description | Background In Ethiopian traditional medicine, Rumex nervosus Vahl leaves are used to treat wounds. However, despite traditional claims and supportive in vitro findings, no scientific study has been conducted to evaluate the in vivo wound healing activity of R. nervosus Vahl leaves. Aim of the study To evaluate the wound healing activity of crude extract of the leaves of R. nervosus Vahl and its solvent fractions in mice. Methods R. nervosus Vahl leaves were extracted with 80% methanol, and then the crude extract was fractionated using ethyl acetate, chloroform, and water. Ointments at 5% and 10% strengths were formulated from the crude extract and its fractions. The healing activity of the crude extract was evaluated using linear incision, circular excision, and burn wound models in mice. Additionally, the activity of solvent fractions was assessed using circular excision wounds in mice. Results Treatment of wounds with ointments containing 5% and 10% crude extract showed significantly increased wound contraction rate, shorter epithelialization period, and higher skin-breaking strength (P < 0.05) compared with the negative control. Both 5% and 10% formulations of the aqueous and ethyl acetate fractions significantly increased wound contraction and decreased the period of epithelialization in the excision wound model (p < 0.05), however, the chloroform fraction showed no significant wound healing effect compared with the negative control. Conclusion The 80% methanol crude extract as well as the aqueous and ethyl acetate fractions of R. nervosus Vahl leaves possess wound healing activity as evidenced by improved wound contraction rate and tensile strength and decreased epithelialization period. |
| format | Article |
| id | doaj-art-cb67e82682ce4398b23c5fbb91d144ae |
| institution | Kabale University |
| issn | 2059-5131 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Scars, Burns & Healing |
| spelling | doaj-art-cb67e82682ce4398b23c5fbb91d144ae2025-01-31T16:03:20ZengSAGE PublishingScars, Burns & Healing2059-51312025-01-011110.1177/20595131251316791Evaluation of wound healing activity of the crude extract and solvent fractions of Vahl (Polygonaceae) leaves in miceAbel Andualem0Kefyalew Ayalew Getahun1Bahiru Tenaw Goshu2Yaschilal Muche Belayneh3 Department of Pharmacy, College of Medicine and Health Sciences, , Dessie, Ethiopia Department of Pharmacology, School of Pharmacy, College of Medicine And Health Sciences University of Gondar, Gondar, Ethiopia Department of Anatomy, School of Medicine, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia Department of Pharmacy, College of Medicine and Health Sciences, , Dessie, EthiopiaBackground In Ethiopian traditional medicine, Rumex nervosus Vahl leaves are used to treat wounds. However, despite traditional claims and supportive in vitro findings, no scientific study has been conducted to evaluate the in vivo wound healing activity of R. nervosus Vahl leaves. Aim of the study To evaluate the wound healing activity of crude extract of the leaves of R. nervosus Vahl and its solvent fractions in mice. Methods R. nervosus Vahl leaves were extracted with 80% methanol, and then the crude extract was fractionated using ethyl acetate, chloroform, and water. Ointments at 5% and 10% strengths were formulated from the crude extract and its fractions. The healing activity of the crude extract was evaluated using linear incision, circular excision, and burn wound models in mice. Additionally, the activity of solvent fractions was assessed using circular excision wounds in mice. Results Treatment of wounds with ointments containing 5% and 10% crude extract showed significantly increased wound contraction rate, shorter epithelialization period, and higher skin-breaking strength (P < 0.05) compared with the negative control. Both 5% and 10% formulations of the aqueous and ethyl acetate fractions significantly increased wound contraction and decreased the period of epithelialization in the excision wound model (p < 0.05), however, the chloroform fraction showed no significant wound healing effect compared with the negative control. Conclusion The 80% methanol crude extract as well as the aqueous and ethyl acetate fractions of R. nervosus Vahl leaves possess wound healing activity as evidenced by improved wound contraction rate and tensile strength and decreased epithelialization period.https://doi.org/10.1177/20595131251316791 |
| spellingShingle | Abel Andualem Kefyalew Ayalew Getahun Bahiru Tenaw Goshu Yaschilal Muche Belayneh Evaluation of wound healing activity of the crude extract and solvent fractions of Vahl (Polygonaceae) leaves in mice Scars, Burns & Healing |
| title | Evaluation of wound healing activity of the crude extract and solvent fractions of Vahl (Polygonaceae) leaves in mice |
| title_full | Evaluation of wound healing activity of the crude extract and solvent fractions of Vahl (Polygonaceae) leaves in mice |
| title_fullStr | Evaluation of wound healing activity of the crude extract and solvent fractions of Vahl (Polygonaceae) leaves in mice |
| title_full_unstemmed | Evaluation of wound healing activity of the crude extract and solvent fractions of Vahl (Polygonaceae) leaves in mice |
| title_short | Evaluation of wound healing activity of the crude extract and solvent fractions of Vahl (Polygonaceae) leaves in mice |
| title_sort | evaluation of wound healing activity of the crude extract and solvent fractions of vahl polygonaceae leaves in mice |
| url | https://doi.org/10.1177/20595131251316791 |
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