Design and In Vitro Biological Evaluation of a Novel Organotin(IV) Complex with 1-(4-Carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione
A novel triphenyltin(IV) compound with 1-(4-carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione was synthesized and characterized by IR, NMR spectroscopy, mass spectrometry, and elemental analysis. In vitro anticancer activity of ligand precursor and synthesized organotin(IV) compound was determine...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/2905840 |
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| author | Nebojša Đ. Pantelić Bojana B. Zmejkovski Željko Žižak Nebojša R. Banjac Bojan Đ. Božić Tatjana P. Stanojković Goran N. Kaluđerović |
| author_facet | Nebojša Đ. Pantelić Bojana B. Zmejkovski Željko Žižak Nebojša R. Banjac Bojan Đ. Božić Tatjana P. Stanojković Goran N. Kaluđerović |
| author_sort | Nebojša Đ. Pantelić |
| collection | DOAJ |
| description | A novel triphenyltin(IV) compound with 1-(4-carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione was synthesized and characterized by IR, NMR spectroscopy, mass spectrometry, and elemental analysis. In vitro anticancer activity of ligand precursor and synthesized organotin(IV) compound was determined against tumor cell lines: human adenocarcinoma (HeLa), human myelogenous leukemia (K562), and human breast cancer (MDA-MB-453), using microculture tetrazolium test (MTT) assay. The results indicate that complex exhibited very high antiproliferative activity against all tested cell lines with IC50 values in the range of 0.22 to 0.53 µM. The highest activity organotin(IV) compound expressed against the HeLa cells (IC50 = 0.22 ± 0.04 µM). The ligand precursor did not show anticancer activity (IC50 > 200 µM). Furthermore, fluorescence microscopy analysis of HeLa cells reveal that organotin(IV) complex induced apoptosis as a mode of cell death, which is consistent with the increase of cells in the sub-G1 phase. |
| format | Article |
| id | doaj-art-cb50f9e56a734670b18b45d872c7c45b |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-cb50f9e56a734670b18b45d872c7c45b2025-02-03T01:03:10ZengWileyJournal of Chemistry2090-90632090-90712019-01-01201910.1155/2019/29058402905840Design and In Vitro Biological Evaluation of a Novel Organotin(IV) Complex with 1-(4-Carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dioneNebojša Đ. Pantelić0Bojana B. Zmejkovski1Željko Žižak2Nebojša R. Banjac3Bojan Đ. Božić4Tatjana P. Stanojković5Goran N. Kaluđerović6Department of Engineering and Natural Sciences, University of Applied Sciences Merseburg, Eberhard-Leibnitz-Strasse 2, 06217 Merseburg, GermanyDepartment of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Studentski Trg 14, 11000 Belgrade, SerbiaInstitute of Oncology and Radiology, 11000 Belgrade, SerbiaDepartment of Chemistry and Biochemistry, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11000 Belgrade-Zemun, SerbiaInstitute of Physiology and Biochemistry, Faculty of Biology, University of Belgrade, Studentski Trg 16, 11000 Belgrade, SerbiaInstitute of Oncology and Radiology, 11000 Belgrade, SerbiaDepartment of Engineering and Natural Sciences, University of Applied Sciences Merseburg, Eberhard-Leibnitz-Strasse 2, 06217 Merseburg, GermanyA novel triphenyltin(IV) compound with 1-(4-carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione was synthesized and characterized by IR, NMR spectroscopy, mass spectrometry, and elemental analysis. In vitro anticancer activity of ligand precursor and synthesized organotin(IV) compound was determined against tumor cell lines: human adenocarcinoma (HeLa), human myelogenous leukemia (K562), and human breast cancer (MDA-MB-453), using microculture tetrazolium test (MTT) assay. The results indicate that complex exhibited very high antiproliferative activity against all tested cell lines with IC50 values in the range of 0.22 to 0.53 µM. The highest activity organotin(IV) compound expressed against the HeLa cells (IC50 = 0.22 ± 0.04 µM). The ligand precursor did not show anticancer activity (IC50 > 200 µM). Furthermore, fluorescence microscopy analysis of HeLa cells reveal that organotin(IV) complex induced apoptosis as a mode of cell death, which is consistent with the increase of cells in the sub-G1 phase.http://dx.doi.org/10.1155/2019/2905840 |
| spellingShingle | Nebojša Đ. Pantelić Bojana B. Zmejkovski Željko Žižak Nebojša R. Banjac Bojan Đ. Božić Tatjana P. Stanojković Goran N. Kaluđerović Design and In Vitro Biological Evaluation of a Novel Organotin(IV) Complex with 1-(4-Carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione Journal of Chemistry |
| title | Design and In Vitro Biological Evaluation of a Novel Organotin(IV) Complex with 1-(4-Carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione |
| title_full | Design and In Vitro Biological Evaluation of a Novel Organotin(IV) Complex with 1-(4-Carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione |
| title_fullStr | Design and In Vitro Biological Evaluation of a Novel Organotin(IV) Complex with 1-(4-Carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione |
| title_full_unstemmed | Design and In Vitro Biological Evaluation of a Novel Organotin(IV) Complex with 1-(4-Carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione |
| title_short | Design and In Vitro Biological Evaluation of a Novel Organotin(IV) Complex with 1-(4-Carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione |
| title_sort | design and in vitro biological evaluation of a novel organotin iv complex with 1 4 carboxyphenyl 3 ethyl 3 methylpyrrolidine 2 5 dione |
| url | http://dx.doi.org/10.1155/2019/2905840 |
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