Healthcare-associated infections caused by Candida spp. in critical infants: a look at environmental surfaces
Background and Objectives: invasive fungal infections entail high morbidity and mortality rates in Neonatal Intensive Care Units (NICUs) and are accompanied by an increasing prevalence of resistant isolates, highlighting hospital environments as the primary sources of contamination. This study ident...
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| Format: | Article |
| Language: | Portuguese |
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Universidade de Santa Cruz do Sul
2025-01-01
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| Series: | Revista de Epidemiologia e Controle de Infecção |
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| Online Access: | https://online.unisc.br/seer/index.php/epidemiologia/article/view/19358 |
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| author | Priscila Guerino Vilela Isadora Caixeta da Silveira Ferreira Ralciane de Paula Menezes Mário Paulo Amante Penatti Reginaldo dos Santos Pedroso Denise Von Dolinger de Brito Röder |
| author_facet | Priscila Guerino Vilela Isadora Caixeta da Silveira Ferreira Ralciane de Paula Menezes Mário Paulo Amante Penatti Reginaldo dos Santos Pedroso Denise Von Dolinger de Brito Röder |
| author_sort | Priscila Guerino Vilela |
| collection | DOAJ |
| description | Background and Objectives: invasive fungal infections entail high morbidity and mortality rates in Neonatal Intensive Care Units (NICUs) and are accompanied by an increasing prevalence of resistant isolates, highlighting hospital environments as the primary sources of contamination. This study identified Candida species in infants in a Brazilian NICU, assessed their clinical and laboratory conditions and characterized the isolates. Methods: Candida isolates from newborns (NBs) and environments were identified and analyzed for antifungal resistance, virulence factors, and molecular relationships. Results: four NBs presented invasive candidiasis, such as C. albicans (2 NBs), C. glabrata (1 NB), and C. parapsilosis sensu stricto (1 NB). All NBs were extremely premature (<29 weeks) and had used at least one invasive device. Two clinical isolates demonstrated resistance, one to fluconazole (C. parapsilosis sensu stricto) and the other to micafungin (C. glabrata). Five environmental isolates were identified as C. parapsilosis sensu stricto, and one of them showed to be fluconazole susceptible-dose dependent. Biofilm was the only virulence factor produced by all nine isolates. Molecular analysis revealed high similarity between one environmental isolate and one clinical isolate of C. parapsilosis sensu stricto. Conclusions: the results indicated the presence of Candida species in infants and NICU environments, with some demonstrating in vitro resistance to fluconazole and micafungin. All isolates produced biofilm. A notable genetic similarity was observed between some environmental and clinical isolates, suggesting the environment as a possible source of infection. |
| format | Article |
| id | doaj-art-cb4618f6568c418f99687048ed6a7cae |
| institution | Kabale University |
| issn | 2238-3360 |
| language | Portuguese |
| publishDate | 2025-01-01 |
| publisher | Universidade de Santa Cruz do Sul |
| record_format | Article |
| series | Revista de Epidemiologia e Controle de Infecção |
| spelling | doaj-art-cb4618f6568c418f99687048ed6a7cae2025-01-20T19:22:55ZporUniversidade de Santa Cruz do SulRevista de Epidemiologia e Controle de Infecção2238-33602025-01-0114410.17058/reci.v14i4.1935818732Healthcare-associated infections caused by Candida spp. in critical infants: a look at environmental surfacesPriscila Guerino VilelaIsadora Caixeta da Silveira FerreiraRalciane de Paula MenezesMário Paulo Amante PenattiReginaldo dos Santos PedrosoDenise Von Dolinger de Brito RöderBackground and Objectives: invasive fungal infections entail high morbidity and mortality rates in Neonatal Intensive Care Units (NICUs) and are accompanied by an increasing prevalence of resistant isolates, highlighting hospital environments as the primary sources of contamination. This study identified Candida species in infants in a Brazilian NICU, assessed their clinical and laboratory conditions and characterized the isolates. Methods: Candida isolates from newborns (NBs) and environments were identified and analyzed for antifungal resistance, virulence factors, and molecular relationships. Results: four NBs presented invasive candidiasis, such as C. albicans (2 NBs), C. glabrata (1 NB), and C. parapsilosis sensu stricto (1 NB). All NBs were extremely premature (<29 weeks) and had used at least one invasive device. Two clinical isolates demonstrated resistance, one to fluconazole (C. parapsilosis sensu stricto) and the other to micafungin (C. glabrata). Five environmental isolates were identified as C. parapsilosis sensu stricto, and one of them showed to be fluconazole susceptible-dose dependent. Biofilm was the only virulence factor produced by all nine isolates. Molecular analysis revealed high similarity between one environmental isolate and one clinical isolate of C. parapsilosis sensu stricto. Conclusions: the results indicated the presence of Candida species in infants and NICU environments, with some demonstrating in vitro resistance to fluconazole and micafungin. All isolates produced biofilm. A notable genetic similarity was observed between some environmental and clinical isolates, suggesting the environment as a possible source of infection.https://online.unisc.br/seer/index.php/epidemiologia/article/view/19358invasive fungal infectionscross infectioninfection controlinfant health |
| spellingShingle | Priscila Guerino Vilela Isadora Caixeta da Silveira Ferreira Ralciane de Paula Menezes Mário Paulo Amante Penatti Reginaldo dos Santos Pedroso Denise Von Dolinger de Brito Röder Healthcare-associated infections caused by Candida spp. in critical infants: a look at environmental surfaces Revista de Epidemiologia e Controle de Infecção invasive fungal infections cross infection infection control infant health |
| title | Healthcare-associated infections caused by Candida spp. in critical infants: a look at environmental surfaces |
| title_full | Healthcare-associated infections caused by Candida spp. in critical infants: a look at environmental surfaces |
| title_fullStr | Healthcare-associated infections caused by Candida spp. in critical infants: a look at environmental surfaces |
| title_full_unstemmed | Healthcare-associated infections caused by Candida spp. in critical infants: a look at environmental surfaces |
| title_short | Healthcare-associated infections caused by Candida spp. in critical infants: a look at environmental surfaces |
| title_sort | healthcare associated infections caused by candida spp in critical infants a look at environmental surfaces |
| topic | invasive fungal infections cross infection infection control infant health |
| url | https://online.unisc.br/seer/index.php/epidemiologia/article/view/19358 |
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