Effects of nanosilicate functionalized polycaprolactone membrane on bone mesenchymal stem cells-induced bone repairing
Objective To fabricate nanosilicate functionalized polycaprolactone (PCL/LAP) electrospun membrane and evaluate its role in bone marrow mesenchymal stem cells (BMSCs)-induced bone repairing. Methods The PCL/LAP electrospun membranes were fabricated via electrospinning technology and co-cultured with...
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Editorial Office of Stomatology
2025-08-01
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| Series: | Kouqiang yixue |
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| Online Access: | https://www.stomatology.cn/fileup/1003-9872/PDF/1755742331545-952851351.pdf |
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| author | XIAO Long, HU Weiqiang, LIN Xuxin, HE Mengjiao, LUO Kai, XU Xiongcheng |
| author_facet | XIAO Long, HU Weiqiang, LIN Xuxin, HE Mengjiao, LUO Kai, XU Xiongcheng |
| author_sort | XIAO Long, HU Weiqiang, LIN Xuxin, HE Mengjiao, LUO Kai, XU Xiongcheng |
| collection | DOAJ |
| description | Objective To fabricate nanosilicate functionalized polycaprolactone (PCL/LAP) electrospun membrane and evaluate its role in bone marrow mesenchymal stem cells (BMSCs)-induced bone repairing. Methods The PCL/LAP electrospun membranes were fabricated via electrospinning technology and co-cultured with rat BMSCs. The cytocompatibility of the membranes was evaluated through cytoskeleton staining, live/dead cell staining and CCK-8 assay. The migration capacity of BMSCs was assessed using scratch assay, Transwell migration experiments and expression of migration-related genes (Pdgf and Tgfβ) was evaluated by qRT-PCR. The osteogenic differentiation and pro-angiogenesis potential were determined by alkaline phosphatase (ALP) staining, alizarin red staining, expression levels of osteogenesis-related genes (Alp, Col1a1, Runx2, Bglap and Bmp2) and angiogenesis-related genes (Angpt1, Fgf2 and Vegfa) along with RUNX2 protein expression. PCL and PCL/LAP electrospun membranes conditioned medium was subsequently used to stimulate vascular endothelial cells (EAhy926). The expression of angiogenesis-associated genes (KDR, ENOS and HIF1A) was quantified by qRT-PCR. Results BMSCs adhered well to the surface of the PCL/LAP membranes, with no significant impact on cell viability (P>0.05). PCL/LAP membranes not only promoted the proliferation (P<0.05), migration (P<0.05), but also enhanced ALP activity and mineralized nodule formation (P<0.05), increased osteogenic differentiation gene and protein expression (P<0.05) of BMSCs. Moreover, PCL/LAP promoted the expression of angiogenic genes of BMSCs (P<0.05), to indirectly regulate angiogenesis-related gene expression in vascular endothelial cells (P<0.05). Conclusion PCL/LAP electrospun membranes exhibit excellent biocompatibility and can promote proliferation, migration, osteogenic differentiation and BMSC-mediated angiogenic differentiation, showing great potential for bone defect repairing as barrier membrane. |
| format | Article |
| id | doaj-art-cb43181f101d4e4e8357767924b9e92f |
| institution | Kabale University |
| issn | 1003-9872 |
| language | zho |
| publishDate | 2025-08-01 |
| publisher | Editorial Office of Stomatology |
| record_format | Article |
| series | Kouqiang yixue |
| spelling | doaj-art-cb43181f101d4e4e8357767924b9e92f2025-08-26T06:49:16ZzhoEditorial Office of StomatologyKouqiang yixue1003-98722025-08-0145856757510.13591/j.cnki.kqyx.2025.08.002Effects of nanosilicate functionalized polycaprolactone membrane on bone mesenchymal stem cells-induced bone repairingXIAO Long, HU Weiqiang, LIN Xuxin, HE Mengjiao, LUO Kai, XU Xiongcheng0 Fujian Key Laboratory of Oral Diseases, Fujian Provincial Engineering Research Center of Oral Biomaterial, Stomatological Key Laboratory of Fujian College and University, Institute of Stomatology & Laboratory of Oral Tissue Engineering, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, ChinaObjective To fabricate nanosilicate functionalized polycaprolactone (PCL/LAP) electrospun membrane and evaluate its role in bone marrow mesenchymal stem cells (BMSCs)-induced bone repairing. Methods The PCL/LAP electrospun membranes were fabricated via electrospinning technology and co-cultured with rat BMSCs. The cytocompatibility of the membranes was evaluated through cytoskeleton staining, live/dead cell staining and CCK-8 assay. The migration capacity of BMSCs was assessed using scratch assay, Transwell migration experiments and expression of migration-related genes (Pdgf and Tgfβ) was evaluated by qRT-PCR. The osteogenic differentiation and pro-angiogenesis potential were determined by alkaline phosphatase (ALP) staining, alizarin red staining, expression levels of osteogenesis-related genes (Alp, Col1a1, Runx2, Bglap and Bmp2) and angiogenesis-related genes (Angpt1, Fgf2 and Vegfa) along with RUNX2 protein expression. PCL and PCL/LAP electrospun membranes conditioned medium was subsequently used to stimulate vascular endothelial cells (EAhy926). The expression of angiogenesis-associated genes (KDR, ENOS and HIF1A) was quantified by qRT-PCR. Results BMSCs adhered well to the surface of the PCL/LAP membranes, with no significant impact on cell viability (P>0.05). PCL/LAP membranes not only promoted the proliferation (P<0.05), migration (P<0.05), but also enhanced ALP activity and mineralized nodule formation (P<0.05), increased osteogenic differentiation gene and protein expression (P<0.05) of BMSCs. Moreover, PCL/LAP promoted the expression of angiogenic genes of BMSCs (P<0.05), to indirectly regulate angiogenesis-related gene expression in vascular endothelial cells (P<0.05). Conclusion PCL/LAP electrospun membranes exhibit excellent biocompatibility and can promote proliferation, migration, osteogenic differentiation and BMSC-mediated angiogenic differentiation, showing great potential for bone defect repairing as barrier membrane.https://www.stomatology.cn/fileup/1003-9872/PDF/1755742331545-952851351.pdf|barrier membrane|polycaprolactone|nanosilicate|bone marrow mesenchymal stem cells|bone regeneration |
| spellingShingle | XIAO Long, HU Weiqiang, LIN Xuxin, HE Mengjiao, LUO Kai, XU Xiongcheng Effects of nanosilicate functionalized polycaprolactone membrane on bone mesenchymal stem cells-induced bone repairing Kouqiang yixue |barrier membrane|polycaprolactone|nanosilicate|bone marrow mesenchymal stem cells|bone regeneration |
| title | Effects of nanosilicate functionalized polycaprolactone membrane on bone mesenchymal stem cells-induced bone repairing |
| title_full | Effects of nanosilicate functionalized polycaprolactone membrane on bone mesenchymal stem cells-induced bone repairing |
| title_fullStr | Effects of nanosilicate functionalized polycaprolactone membrane on bone mesenchymal stem cells-induced bone repairing |
| title_full_unstemmed | Effects of nanosilicate functionalized polycaprolactone membrane on bone mesenchymal stem cells-induced bone repairing |
| title_short | Effects of nanosilicate functionalized polycaprolactone membrane on bone mesenchymal stem cells-induced bone repairing |
| title_sort | effects of nanosilicate functionalized polycaprolactone membrane on bone mesenchymal stem cells induced bone repairing |
| topic | |barrier membrane|polycaprolactone|nanosilicate|bone marrow mesenchymal stem cells|bone regeneration |
| url | https://www.stomatology.cn/fileup/1003-9872/PDF/1755742331545-952851351.pdf |
| work_keys_str_mv | AT xiaolonghuweiqianglinxuxinhemengjiaoluokaixuxiongcheng effectsofnanosilicatefunctionalizedpolycaprolactonemembraneonbonemesenchymalstemcellsinducedbonerepairing |