miR-223 promotes cartilage differentiation of bone marrow-derived mesenchymal stem cells and protects against osteoarthritis by suppressing NLRP-3 expression
Introduction The present investigation evaluates the role of miR-223 mimic in the treatment of osteoarthritis (OA) and postulates the possible molecular mechanism of its action. Material and methods Bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from rats and cultured in chondroge...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2020-11-01
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| Series: | Archives of Medical Science |
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| Online Access: | https://www.archivesofmedicalscience.com/miR-223-promotes-cartilage-differentiation-nof-bone-marrow-derived-mesenchymal-stem,127271,0,2.html |
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| author | Nan Min Jie Ma Lei Shi Lin Wang Chi Liu Yaonan Zhang Qingyun Xue |
| author_facet | Nan Min Jie Ma Lei Shi Lin Wang Chi Liu Yaonan Zhang Qingyun Xue |
| author_sort | Nan Min |
| collection | DOAJ |
| description | Introduction
The present investigation evaluates the role of miR-223 mimic in the treatment of osteoarthritis (OA) and postulates the possible molecular mechanism of its action.
Material and methods
Bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from rats and cultured in chondrogenic medium to stimulate the differentiation of chondrocytes. Alcian blue staining was performed to determine the chondrogenic differentiation and expression of miR-223 in the BMSCs. Moreover, expression of NLR family pyrin domain containing 3 (NLRP-3), matrix metallopeptidase-13 (MMP-13) and collagen (Col II) was determined in miR-223 mimic and inhibitor treated BMSCs. OA was induced by injecting anterior cruciate ligament transection in rats followed by further treatment with the miR-223 mimic for the period of the treatment protocol. Level and expression of inflammatory cytokines were estimated in the cartilage tissue of OA rats. Moreover, immunohistochemical analysis and histopathology study were also performed.
Results
Data of the study reveal that expression of miR-223 was higher in chondrogenic differentiated BMSCs than normal. Expression of MMP-13 and NLRP-3 was lower, and expression of Col II was higher in miR-223 mimic treated BMSCs than normal. Moreover, data of the in-vivo study indicate that the expression level of cytokines was lower in the cartilage tissue of the miR-223 mimic treated group than the OA group. Treatment with the miR-223 mimic ameliorates the altered histopathology and expression of NLRP-3 in the cartilage tissue of OA rats.
Conclusions
Data of the study reveal that the miR-223 mimic enhances the chondrogenic differentiation of BMSCs by regulating the NLRP-3/IL-18/TGF-β pathway. |
| format | Article |
| id | doaj-art-cb348f11f19d4b36956cf59007b5259d |
| institution | Kabale University |
| issn | 1734-1922 1896-9151 |
| language | English |
| publishDate | 2020-11-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj-art-cb348f11f19d4b36956cf59007b5259d2025-01-27T10:45:12ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512020-11-012062002200810.5114/aoms.2020.100640127271miR-223 promotes cartilage differentiation of bone marrow-derived mesenchymal stem cells and protects against osteoarthritis by suppressing NLRP-3 expressionNan Min0Jie Ma1Lei Shi2Lin Wang3Chi Liu4Yaonan Zhang5Qingyun Xue6Department of Orthopedics, Beijing Hospital, Beijing, ChinaCenter of Biotherapy, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, ChinaDepartment of Orthopedics, Beijing Hospital, Beijing, ChinaDepartment of Orthopedics, Beijing Hospital, Beijing, ChinaDepartment of Orthopedics, Beijing Hospital, Beijing, ChinaDepartment of Orthopedics, Beijing Hospital, Beijing, ChinaDepartment of Orthopedics, Beijing Hospital, Beijing, ChinaIntroduction The present investigation evaluates the role of miR-223 mimic in the treatment of osteoarthritis (OA) and postulates the possible molecular mechanism of its action. Material and methods Bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from rats and cultured in chondrogenic medium to stimulate the differentiation of chondrocytes. Alcian blue staining was performed to determine the chondrogenic differentiation and expression of miR-223 in the BMSCs. Moreover, expression of NLR family pyrin domain containing 3 (NLRP-3), matrix metallopeptidase-13 (MMP-13) and collagen (Col II) was determined in miR-223 mimic and inhibitor treated BMSCs. OA was induced by injecting anterior cruciate ligament transection in rats followed by further treatment with the miR-223 mimic for the period of the treatment protocol. Level and expression of inflammatory cytokines were estimated in the cartilage tissue of OA rats. Moreover, immunohistochemical analysis and histopathology study were also performed. Results Data of the study reveal that expression of miR-223 was higher in chondrogenic differentiated BMSCs than normal. Expression of MMP-13 and NLRP-3 was lower, and expression of Col II was higher in miR-223 mimic treated BMSCs than normal. Moreover, data of the in-vivo study indicate that the expression level of cytokines was lower in the cartilage tissue of the miR-223 mimic treated group than the OA group. Treatment with the miR-223 mimic ameliorates the altered histopathology and expression of NLRP-3 in the cartilage tissue of OA rats. Conclusions Data of the study reveal that the miR-223 mimic enhances the chondrogenic differentiation of BMSCs by regulating the NLRP-3/IL-18/TGF-β pathway.https://www.archivesofmedicalscience.com/miR-223-promotes-cartilage-differentiation-nof-bone-marrow-derived-mesenchymal-stem,127271,0,2.htmlmir-223osteoarthritisbone marrow-derived mesenchymal stem cellscartilageinflammation |
| spellingShingle | Nan Min Jie Ma Lei Shi Lin Wang Chi Liu Yaonan Zhang Qingyun Xue miR-223 promotes cartilage differentiation of bone marrow-derived mesenchymal stem cells and protects against osteoarthritis by suppressing NLRP-3 expression Archives of Medical Science mir-223 osteoarthritis bone marrow-derived mesenchymal stem cells cartilage inflammation |
| title | miR-223 promotes cartilage differentiation
of bone marrow-derived mesenchymal stem cells
and protects against osteoarthritis by suppressing
NLRP-3 expression |
| title_full | miR-223 promotes cartilage differentiation
of bone marrow-derived mesenchymal stem cells
and protects against osteoarthritis by suppressing
NLRP-3 expression |
| title_fullStr | miR-223 promotes cartilage differentiation
of bone marrow-derived mesenchymal stem cells
and protects against osteoarthritis by suppressing
NLRP-3 expression |
| title_full_unstemmed | miR-223 promotes cartilage differentiation
of bone marrow-derived mesenchymal stem cells
and protects against osteoarthritis by suppressing
NLRP-3 expression |
| title_short | miR-223 promotes cartilage differentiation
of bone marrow-derived mesenchymal stem cells
and protects against osteoarthritis by suppressing
NLRP-3 expression |
| title_sort | mir 223 promotes cartilage differentiation of bone marrow derived mesenchymal stem cells and protects against osteoarthritis by suppressing nlrp 3 expression |
| topic | mir-223 osteoarthritis bone marrow-derived mesenchymal stem cells cartilage inflammation |
| url | https://www.archivesofmedicalscience.com/miR-223-promotes-cartilage-differentiation-nof-bone-marrow-derived-mesenchymal-stem,127271,0,2.html |
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