Role of HO/CO in the Control of Peripheral Circulation in Humans
Experimental studies show that the heme oxygenase/carbon monoxide system (HO/CO) plays an important role in the homeostasis of circulation and in the pathophysiology of hypertension. No data are available on its role in the control of peripheral circulation in humans. We evaluated the effects of inh...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Hypertension |
| Online Access: | http://dx.doi.org/10.1155/2012/236180 |
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| author | David Sacerdoti Despina Mania Paola Pesce Silvia Gaiani Angelo Gatta Massimo Bolognesi |
| author_facet | David Sacerdoti Despina Mania Paola Pesce Silvia Gaiani Angelo Gatta Massimo Bolognesi |
| author_sort | David Sacerdoti |
| collection | DOAJ |
| description | Experimental studies show that the heme oxygenase/carbon monoxide system (HO/CO) plays an important role in the homeostasis of circulation and in the pathophysiology of hypertension. No data are available on its role in the control of peripheral circulation in humans. We evaluated the effects of inhibition of HO with stannous mesoporphyrin IX (SnMP) (200 𝜇M) locally administered by iontophoresis, on human skin blood flow, evaluated by laser-Doppler flowmetry, in the presence and absence of nitric oxide synthase (NOS) inhibition with L-NG-Nitroarginine methyl ester (L-NAME) (100 𝜇M). We also evaluated the effect of HO inhibition on vasodilatation induced by acetylcholine (ACh) and vasoconstriction caused by noradrenaline (NA). SnMP and L-NAME caused a similar 20–25% decrease in skin flow. After nitric oxide (NO) inhibition with L-NAME, HO inhibition with SnMP caused a further 20% decrease in skin perfusion. SnMP decreased vasodilatation induced by ACh by about 70%, while it did not affect vasoconstriction to NA. In conclusion, HO/CO participates in the control of peripheral circulation, independently from NO, and is involved in vasodilatation to ACh. |
| format | Article |
| id | doaj-art-cb1700b5d1104a158dd8a262f83d6c4e |
| institution | Kabale University |
| issn | 2090-0384 2090-0392 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Hypertension |
| spelling | doaj-art-cb1700b5d1104a158dd8a262f83d6c4e2025-02-03T05:53:44ZengWileyInternational Journal of Hypertension2090-03842090-03922012-01-01201210.1155/2012/236180236180Role of HO/CO in the Control of Peripheral Circulation in HumansDavid Sacerdoti0Despina Mania1Paola Pesce2Silvia Gaiani3Angelo Gatta4Massimo Bolognesi5Department of Clinical and Experimental Medicine, Clinica Medica 5, University of Padova, Via Giustiniani 2, 35100 Padova, ItalyDepartment of Clinical and Experimental Medicine, Clinica Medica 5, University of Padova, Via Giustiniani 2, 35100 Padova, ItalyDepartment of Clinical and Experimental Medicine, Clinica Medica 5, University of Padova, Via Giustiniani 2, 35100 Padova, ItalyDepartment of Clinical and Experimental Medicine, Clinica Medica 5, University of Padova, Via Giustiniani 2, 35100 Padova, ItalyDepartment of Clinical and Experimental Medicine, Clinica Medica 5, University of Padova, Via Giustiniani 2, 35100 Padova, ItalyDepartment of Clinical and Experimental Medicine, Clinica Medica 5, University of Padova, Via Giustiniani 2, 35100 Padova, ItalyExperimental studies show that the heme oxygenase/carbon monoxide system (HO/CO) plays an important role in the homeostasis of circulation and in the pathophysiology of hypertension. No data are available on its role in the control of peripheral circulation in humans. We evaluated the effects of inhibition of HO with stannous mesoporphyrin IX (SnMP) (200 𝜇M) locally administered by iontophoresis, on human skin blood flow, evaluated by laser-Doppler flowmetry, in the presence and absence of nitric oxide synthase (NOS) inhibition with L-NG-Nitroarginine methyl ester (L-NAME) (100 𝜇M). We also evaluated the effect of HO inhibition on vasodilatation induced by acetylcholine (ACh) and vasoconstriction caused by noradrenaline (NA). SnMP and L-NAME caused a similar 20–25% decrease in skin flow. After nitric oxide (NO) inhibition with L-NAME, HO inhibition with SnMP caused a further 20% decrease in skin perfusion. SnMP decreased vasodilatation induced by ACh by about 70%, while it did not affect vasoconstriction to NA. In conclusion, HO/CO participates in the control of peripheral circulation, independently from NO, and is involved in vasodilatation to ACh.http://dx.doi.org/10.1155/2012/236180 |
| spellingShingle | David Sacerdoti Despina Mania Paola Pesce Silvia Gaiani Angelo Gatta Massimo Bolognesi Role of HO/CO in the Control of Peripheral Circulation in Humans International Journal of Hypertension |
| title | Role of HO/CO in the Control of Peripheral Circulation in Humans |
| title_full | Role of HO/CO in the Control of Peripheral Circulation in Humans |
| title_fullStr | Role of HO/CO in the Control of Peripheral Circulation in Humans |
| title_full_unstemmed | Role of HO/CO in the Control of Peripheral Circulation in Humans |
| title_short | Role of HO/CO in the Control of Peripheral Circulation in Humans |
| title_sort | role of ho co in the control of peripheral circulation in humans |
| url | http://dx.doi.org/10.1155/2012/236180 |
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