Adsorption and Desorption Characteristics and Purification of Isoflavones from Crude Soybean Extract Using Macroporous Resins
Isoflavones in soybean have been well-known with many health-promoting effects on humans. This study aimed to purify isoflavones from the crude soybean extract by the static adsorption/desorption process on macroporous resins. A screening test of four commercial resins: D101, AB-8, Amberlite (R) XA...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2022-06-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://journal.pan.olsztyn.pl/Adsorption-and-Desorption-Characteristics-and-Purification-of-Isoflavones-from-Crude,149816,0,2.html |
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| author | Thi Ngoc Thu Tran Xuan Vung Bui Nguyen Thi Truc Loan Nguyen Huu Thuan Anh Truong Dang Le Thi Minh Hanh Truong |
| author_facet | Thi Ngoc Thu Tran Xuan Vung Bui Nguyen Thi Truc Loan Nguyen Huu Thuan Anh Truong Dang Le Thi Minh Hanh Truong |
| author_sort | Thi Ngoc Thu Tran |
| collection | DOAJ |
| description | Isoflavones in soybean have been well-known with many health-promoting effects on humans. This study aimed to purify isoflavones from the crude soybean extract by the static adsorption/desorption process on macroporous resins. A screening test of four commercial resins: D101, AB-8, Amberlite (R) XAD4, and Diaion HP20 according to their adsorption/desorption characteristic for isoflavones was investigated. All four resins showed high adsorption and desorption characteristics in which D101 resin was chosen as the most suitable resin for purifying isoflavones. Compositional analysis showed that daidzin and genistin were the main isoflavones in the crude soybean extract. The adsorption isotherms data of total isoflavones, daidzin, and genistin fitted well with the Langmuir model with R 2 >0.98. The dynamic adsorption conditions for the purification process of isoflavones on the D101 resin-packed column were selected at the bed volume (BV) of 200 mL, feed volume of 3.75 BV, and flow rate of 1.5 BV/h. The dynamic desorption was carried out with the elution solution of 70% ( v/v ) ethanol, elution volume of 2.5 BV, and flow rate of 1 BV/h. The total isoflavone content in the purified extract was 8.70-fold higher than its initial content in the crude soybean extract with a recovery yield of nearly 80%. The study results reveal a strong possibility for large-scale production of isoflavones for further application in functional food products or pharmaceutical products. |
| format | Article |
| id | doaj-art-caf25ae0694b4522bf0abda086787892 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2022-06-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-caf25ae0694b4522bf0abda0867878922025-02-03T02:00:30ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072022-06-0172218319210.31883/pjfns/149816149816Adsorption and Desorption Characteristics and Purification of Isoflavones from Crude Soybean Extract Using Macroporous ResinsThi Ngoc Thu Tran0Xuan Vung Bui1Nguyen Thi Truc Loan2https://orcid.org/0000-0002-0152-2981Nguyen Huu Thuan Anh3Truong Dang Le4https://orcid.org/0000-0003-0335-1140Thi Minh Hanh Truong5University of Technology and Education, The University of Danang, 48 Cao Thang St., 550000 Danang, VietnamUniversity of Education, The University of Danang, 459 Ton Duc Thang St., 550000 Danang, VietnamUniversity of Science and Technology, The University of Danang, 54 Nguyen Luong Bang St., 550000 Danang, VietnamInstitute of Environmental Technology and Sustainable Development, Nguyen Tat Thanh University, Ho Chi Minh City 700000, VietnamInstitute of Environmental Technology and Sustainable Development, Nguyen Tat Thanh University, Ho Chi Minh City 700000, VietnamUniversity of Science and Technology, The University of Danang, 54 Nguyen Luong Bang St., 550000 Danang, VietnamIsoflavones in soybean have been well-known with many health-promoting effects on humans. This study aimed to purify isoflavones from the crude soybean extract by the static adsorption/desorption process on macroporous resins. A screening test of four commercial resins: D101, AB-8, Amberlite (R) XAD4, and Diaion HP20 according to their adsorption/desorption characteristic for isoflavones was investigated. All four resins showed high adsorption and desorption characteristics in which D101 resin was chosen as the most suitable resin for purifying isoflavones. Compositional analysis showed that daidzin and genistin were the main isoflavones in the crude soybean extract. The adsorption isotherms data of total isoflavones, daidzin, and genistin fitted well with the Langmuir model with R 2 >0.98. The dynamic adsorption conditions for the purification process of isoflavones on the D101 resin-packed column were selected at the bed volume (BV) of 200 mL, feed volume of 3.75 BV, and flow rate of 1.5 BV/h. The dynamic desorption was carried out with the elution solution of 70% ( v/v ) ethanol, elution volume of 2.5 BV, and flow rate of 1 BV/h. The total isoflavone content in the purified extract was 8.70-fold higher than its initial content in the crude soybean extract with a recovery yield of nearly 80%. The study results reveal a strong possibility for large-scale production of isoflavones for further application in functional food products or pharmaceutical products.http://journal.pan.olsztyn.pl/Adsorption-and-Desorption-Characteristics-and-Purification-of-Isoflavones-from-Crude,149816,0,2.htmlsoybean isoflavonesmacroporous resinpurificationadsorption and desorption characteristicsdaidzingenistin |
| spellingShingle | Thi Ngoc Thu Tran Xuan Vung Bui Nguyen Thi Truc Loan Nguyen Huu Thuan Anh Truong Dang Le Thi Minh Hanh Truong Adsorption and Desorption Characteristics and Purification of Isoflavones from Crude Soybean Extract Using Macroporous Resins Polish Journal of Food and Nutrition Sciences soybean isoflavones macroporous resin purification adsorption and desorption characteristics daidzin genistin |
| title | Adsorption and Desorption Characteristics and Purification of Isoflavones from Crude Soybean Extract Using Macroporous Resins |
| title_full | Adsorption and Desorption Characteristics and Purification of Isoflavones from Crude Soybean Extract Using Macroporous Resins |
| title_fullStr | Adsorption and Desorption Characteristics and Purification of Isoflavones from Crude Soybean Extract Using Macroporous Resins |
| title_full_unstemmed | Adsorption and Desorption Characteristics and Purification of Isoflavones from Crude Soybean Extract Using Macroporous Resins |
| title_short | Adsorption and Desorption Characteristics and Purification of Isoflavones from Crude Soybean Extract Using Macroporous Resins |
| title_sort | adsorption and desorption characteristics and purification of isoflavones from crude soybean extract using macroporous resins |
| topic | soybean isoflavones macroporous resin purification adsorption and desorption characteristics daidzin genistin |
| url | http://journal.pan.olsztyn.pl/Adsorption-and-Desorption-Characteristics-and-Purification-of-Isoflavones-from-Crude,149816,0,2.html |
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