New insights into the N-glycomes of Dictyostelium species
Dictyostelia are cellular slime molds, a group of Amoebozoa, that form multicellular fruiting bodies out of aggregating cells able of differentiating into resistant spore forms. In previous studies on Dictyostelium discoideum, it was demonstrated that their N-glycans, as in most eukaryotes, derive f...
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Elsevier
2025-01-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667160325000055 |
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| author | Alba Hykollari Daniel Malzl Chunsheng Jin Carina Eschenbach Kristína Kianičková Iain B.H. Wilson Katharina Paschinger |
| author_facet | Alba Hykollari Daniel Malzl Chunsheng Jin Carina Eschenbach Kristína Kianičková Iain B.H. Wilson Katharina Paschinger |
| author_sort | Alba Hykollari |
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| description | Dictyostelia are cellular slime molds, a group of Amoebozoa, that form multicellular fruiting bodies out of aggregating cells able of differentiating into resistant spore forms. In previous studies on Dictyostelium discoideum, it was demonstrated that their N-glycans, as in most eukaryotes, derive from the Glc3Man9GlcNAc2-PP-Dol precursor; however, unique glyco-epitopes, including intersecting GlcNAc, core α1,3-fucosylation, sulphation and methylphosphorylation, were detected. In the present study, we have examined the N-glycans of two other Dictyostelium species, D. purpureum, whose genome is also sequenced, and D. giganteum. The detailed glycomic analysis of their fruiting bodies was based on isomeric separation of the glycan structures by HPLC, followed by mass spectrometry in combination with enzymatic digests and chemical treatments. Two features absent from the 'model' dictyostelid D. discoideum were found: especially in D. purpureum, a long linear galactose arm β1,4-linked to the β1,4-N-acetylglucosamine on the 'lower' A-branch of its oligo-mannosylated structures could be identified. In contrast, neutral N-glycans with multiple fucose residues attached to terminal mannoses were found in D. giganteum. All three species have common modifications on their anionic N-glycans: while (methyl)phosphorylated residues are always associated with terminal mannose residues, the sulphation position differs. While D. discoideum has 6-sulphation of subterminal mannose residues, D. giganteum and D. purpureum may rather have 2-sulphation of core α1,6-mannose. Overall, we have discovered species-specific glycan variations and our data will contribute to future comparative and functional studies on these three species within the same genus. |
| format | Article |
| id | doaj-art-cae719e36bde4ac49d8a93d4b477a6e1 |
| institution | Kabale University |
| issn | 2667-1603 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | BBA Advances |
| spelling | doaj-art-cae719e36bde4ac49d8a93d4b477a6e12025-01-24T04:45:53ZengElsevierBBA Advances2667-16032025-01-017100142New insights into the N-glycomes of Dictyostelium speciesAlba Hykollari0Daniel Malzl1Chunsheng Jin2Carina Eschenbach3Kristína Kianičková4Iain B.H. Wilson5Katharina Paschinger6Department für Chemie, Universität für Bodenkultur, 1190 Wien, Austria; Department für Interdisziplinäre Lebenswissenschaften, Veterinärmedizinische Universität, 1160 Wien, AustriaDepartment für Chemie, Universität für Bodenkultur, 1190 Wien, AustriaInstitutionen för biomedicin, Göteborgs universitet, 405 30 Göteborg, SwedenDepartment für Chemie, Universität für Bodenkultur, 1190 Wien, Austria; Department für Interdisziplinäre Lebenswissenschaften, Veterinärmedizinische Universität, 1160 Wien, AustriaDepartment für Chemie, Universität für Bodenkultur, 1190 Wien, AustriaDepartment für Chemie, Universität für Bodenkultur, 1190 Wien, Austria; Corresponding author.Department für Chemie, Universität für Bodenkultur, 1190 Wien, AustriaDictyostelia are cellular slime molds, a group of Amoebozoa, that form multicellular fruiting bodies out of aggregating cells able of differentiating into resistant spore forms. In previous studies on Dictyostelium discoideum, it was demonstrated that their N-glycans, as in most eukaryotes, derive from the Glc3Man9GlcNAc2-PP-Dol precursor; however, unique glyco-epitopes, including intersecting GlcNAc, core α1,3-fucosylation, sulphation and methylphosphorylation, were detected. In the present study, we have examined the N-glycans of two other Dictyostelium species, D. purpureum, whose genome is also sequenced, and D. giganteum. The detailed glycomic analysis of their fruiting bodies was based on isomeric separation of the glycan structures by HPLC, followed by mass spectrometry in combination with enzymatic digests and chemical treatments. Two features absent from the 'model' dictyostelid D. discoideum were found: especially in D. purpureum, a long linear galactose arm β1,4-linked to the β1,4-N-acetylglucosamine on the 'lower' A-branch of its oligo-mannosylated structures could be identified. In contrast, neutral N-glycans with multiple fucose residues attached to terminal mannoses were found in D. giganteum. All three species have common modifications on their anionic N-glycans: while (methyl)phosphorylated residues are always associated with terminal mannose residues, the sulphation position differs. While D. discoideum has 6-sulphation of subterminal mannose residues, D. giganteum and D. purpureum may rather have 2-sulphation of core α1,6-mannose. Overall, we have discovered species-specific glycan variations and our data will contribute to future comparative and functional studies on these three species within the same genus.http://www.sciencedirect.com/science/article/pii/S2667160325000055DictyosteliaN-glycanFucoseGalactosePhosphateSulphate |
| spellingShingle | Alba Hykollari Daniel Malzl Chunsheng Jin Carina Eschenbach Kristína Kianičková Iain B.H. Wilson Katharina Paschinger New insights into the N-glycomes of Dictyostelium species BBA Advances Dictyostelia N-glycan Fucose Galactose Phosphate Sulphate |
| title | New insights into the N-glycomes of Dictyostelium species |
| title_full | New insights into the N-glycomes of Dictyostelium species |
| title_fullStr | New insights into the N-glycomes of Dictyostelium species |
| title_full_unstemmed | New insights into the N-glycomes of Dictyostelium species |
| title_short | New insights into the N-glycomes of Dictyostelium species |
| title_sort | new insights into the n glycomes of dictyostelium species |
| topic | Dictyostelia N-glycan Fucose Galactose Phosphate Sulphate |
| url | http://www.sciencedirect.com/science/article/pii/S2667160325000055 |
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