Anther transcriptomes in cold-tolerant rice cultivars tend to show insensitive responses
Many studies of stress tolerance in plants have characterized genes that show differences among a small number of lines with clearly distinct tolerance or sensitivity to the given stress. From the few cloned genes, it is difficult to genetically interpret intermediate tolerance or susceptibility lev...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Plant Stress |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X24003531 |
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| author | Koichi Yamamori Seiya Ishiguro Kei Ogasawara Kayyis Muayadah Lubba Kaien Fujino Kazumitsu Onishi Yutaka Sato Yuji Kishima |
| author_facet | Koichi Yamamori Seiya Ishiguro Kei Ogasawara Kayyis Muayadah Lubba Kaien Fujino Kazumitsu Onishi Yutaka Sato Yuji Kishima |
| author_sort | Koichi Yamamori |
| collection | DOAJ |
| description | Many studies of stress tolerance in plants have characterized genes that show differences among a small number of lines with clearly distinct tolerance or sensitivity to the given stress. From the few cloned genes, it is difficult to genetically interpret intermediate tolerance or susceptibility levels and explain the complexity of stress responses and tolerance. In this study, we explored the changes in the transcriptome of anthers from 13 rice lines with different cold tolerance grown under control conditions or exposed to 4 days of cold stress to look for correlations between cold tolerance at the booting stage and expression levels. When examining the overall expression patterns in anthers at low temperature, the cold-tolerant lines tended to have relatively few highly expressed genes, and the expression levels of ribosome-related genes tended to be lower in cold-tolerant lines than in cold-sensitive lines. Importantly, we observed these different expression patterns between the cold-tolerant and -sensitive lines regardless of whether cold stress had been applied. Minimal changes in genes and repetitive sequences under cold stress also tended to be characteristic of the cold-tolerant lines. We also identified unknown genes whose expression was cold responsive and common to all the lines studied. The rice lines whose transcriptome remains constant or insensitive in response to cold stress are more tolerant to low-temperature exposure during the booting stage than rice lines with more widespread expression changes. |
| format | Article |
| id | doaj-art-cae14462aa794c489bfe9ae2d5d7b0dc |
| institution | Kabale University |
| issn | 2667-064X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj-art-cae14462aa794c489bfe9ae2d5d7b0dc2025-02-04T04:10:39ZengElsevierPlant Stress2667-064X2025-03-0115100700Anther transcriptomes in cold-tolerant rice cultivars tend to show insensitive responsesKoichi Yamamori0Seiya Ishiguro1Kei Ogasawara2Kayyis Muayadah Lubba3Kaien Fujino4Kazumitsu Onishi5Yutaka Sato6Yuji Kishima7Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan; Corresponding authors.Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanResearch Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanResearch Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanResearch Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanDepartment of Agro-environmental Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanNational Agriculture and Food Research Organization, Hokkaido Agriculture Research Center, Sapporo 062-8555, JapanResearch Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan; Corresponding authors.Many studies of stress tolerance in plants have characterized genes that show differences among a small number of lines with clearly distinct tolerance or sensitivity to the given stress. From the few cloned genes, it is difficult to genetically interpret intermediate tolerance or susceptibility levels and explain the complexity of stress responses and tolerance. In this study, we explored the changes in the transcriptome of anthers from 13 rice lines with different cold tolerance grown under control conditions or exposed to 4 days of cold stress to look for correlations between cold tolerance at the booting stage and expression levels. When examining the overall expression patterns in anthers at low temperature, the cold-tolerant lines tended to have relatively few highly expressed genes, and the expression levels of ribosome-related genes tended to be lower in cold-tolerant lines than in cold-sensitive lines. Importantly, we observed these different expression patterns between the cold-tolerant and -sensitive lines regardless of whether cold stress had been applied. Minimal changes in genes and repetitive sequences under cold stress also tended to be characteristic of the cold-tolerant lines. We also identified unknown genes whose expression was cold responsive and common to all the lines studied. The rice lines whose transcriptome remains constant or insensitive in response to cold stress are more tolerant to low-temperature exposure during the booting stage than rice lines with more widespread expression changes.http://www.sciencedirect.com/science/article/pii/S2667064X24003531AntherBooting stageCold toleranceFlowering stagePollenRice |
| spellingShingle | Koichi Yamamori Seiya Ishiguro Kei Ogasawara Kayyis Muayadah Lubba Kaien Fujino Kazumitsu Onishi Yutaka Sato Yuji Kishima Anther transcriptomes in cold-tolerant rice cultivars tend to show insensitive responses Plant Stress Anther Booting stage Cold tolerance Flowering stage Pollen Rice |
| title | Anther transcriptomes in cold-tolerant rice cultivars tend to show insensitive responses |
| title_full | Anther transcriptomes in cold-tolerant rice cultivars tend to show insensitive responses |
| title_fullStr | Anther transcriptomes in cold-tolerant rice cultivars tend to show insensitive responses |
| title_full_unstemmed | Anther transcriptomes in cold-tolerant rice cultivars tend to show insensitive responses |
| title_short | Anther transcriptomes in cold-tolerant rice cultivars tend to show insensitive responses |
| title_sort | anther transcriptomes in cold tolerant rice cultivars tend to show insensitive responses |
| topic | Anther Booting stage Cold tolerance Flowering stage Pollen Rice |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X24003531 |
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