Optimizations of Placenta Extracellular Matrix‐Loaded Silk Fibroin/Alginate 3D‐Printed Scaffolds Structurally and Functionally for Bone Tissue Engineering
ABSTRACT Recent interest has been focused on extracellular matrix (ECM)–based scaffolds totreat critical‐sized bone injuries. In this study, urea was used to decellularize and solubilize human placenta tissue. Then, different concentrations of ECM were composited with 8% alginate (Alg) and 12% silk...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2025-01-01
|
| Series: | Engineering in Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/elsc.202400085 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832581985669742592 |
|---|---|
| author | Zahra Bashiri Zahra Khosrowpour Ali Moghaddaszadeh Davod Jafari Sanaz Alizadeh Hajar Nasiri Houman Parsaei Zahra Keshtkaran Meghdad Abdollahpour‐Alitappeh Farshad Bargrizaneh Behzad Rezaei Sara Simorgh Mazaher Gholipourmalekabadi |
| author_facet | Zahra Bashiri Zahra Khosrowpour Ali Moghaddaszadeh Davod Jafari Sanaz Alizadeh Hajar Nasiri Houman Parsaei Zahra Keshtkaran Meghdad Abdollahpour‐Alitappeh Farshad Bargrizaneh Behzad Rezaei Sara Simorgh Mazaher Gholipourmalekabadi |
| author_sort | Zahra Bashiri |
| collection | DOAJ |
| description | ABSTRACT Recent interest has been focused on extracellular matrix (ECM)–based scaffolds totreat critical‐sized bone injuries. In this study, urea was used to decellularize and solubilize human placenta tissue. Then, different concentrations of ECM were composited with 8% alginate (Alg) and 12% silk fibroin (SF) for printing in order to produce a natural 3D construct that resembled bone tissue. The physical and biological features of the printed structures were evaluated entirely in vitro. Finally, a rat model was employed to examine the optimal 3D printed scaffold (5% ECM) as a bone transplant for the healing of cranial bone lesions. The present investigation demonstrated that decellularizing placental tissue fragments led to efficient removal of cell debris. In addition, a remarkable improvement in the printed scaffolds' mechanical and biological properties was observed by increasing the ECM concentration. The histology studies and real‐time PCR results demonstrated the acceleration of bone regeneration in the bone lesions treated with 5%ECM‐SF/Alg at 4 and 8 weeks after implantation. Overall, these results proved that the placental ECM‐printed scaffolds could potentially construct biomimetic grafts to reconstruct significant bone defects and now promise to proceed with clinical studies. |
| format | Article |
| id | doaj-art-cad55aad227042b38a5ffeb13f00bc75 |
| institution | Kabale University |
| issn | 1618-0240 1618-2863 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Engineering in Life Sciences |
| spelling | doaj-art-cad55aad227042b38a5ffeb13f00bc752025-01-30T06:40:30ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632025-01-01251n/an/a10.1002/elsc.202400085Optimizations of Placenta Extracellular Matrix‐Loaded Silk Fibroin/Alginate 3D‐Printed Scaffolds Structurally and Functionally for Bone Tissue EngineeringZahra Bashiri0Zahra Khosrowpour1Ali Moghaddaszadeh2Davod Jafari3Sanaz Alizadeh4Hajar Nasiri5Houman Parsaei6Zahra Keshtkaran7Meghdad Abdollahpour‐Alitappeh8Farshad Bargrizaneh9Behzad Rezaei10Sara Simorgh11Mazaher Gholipourmalekabadi12Endometrium and Endometriosis Research Center Hamadan University of Medical Sciences Hamadan IranDepartment of Pediatrics University of Minnesota Minneapolis Minnesota USADepartement of Biomedical Engineering, Science and Research Branch Islamic Azad University Tehran IranOncopathology Research Center Iran University of Medical Sciences Tehran IranR&D Department Royan Stem Cell Technology Co Tehran IranCellular and Molecular Research Center Iran University of Medical Sciences Tehran IranNervous System Stem Cells Research Center Semnan University of Medical Sciences Semnan IranCommunity Based Psychiatric Care Research Center, Department of Nursing, School of Nursing and Midwifery Shiraz University of Medical Sciences Shiraz IranDepartment of Physiology and Pharmacology Pasteur Institute of Iran Tehran IranStudent Research Committee, School of Health Management and Information Sciences Shiraz Universiy of Medical Sciences Shiraz IranDepartment of Surgery, School of Medicine Larestan University of Medical Sciences Larestan IranCellular and Molecular Research Center Iran University of Medical Sciences Tehran IranCellular and Molecular Research Center Iran University of Medical Sciences Tehran IranABSTRACT Recent interest has been focused on extracellular matrix (ECM)–based scaffolds totreat critical‐sized bone injuries. In this study, urea was used to decellularize and solubilize human placenta tissue. Then, different concentrations of ECM were composited with 8% alginate (Alg) and 12% silk fibroin (SF) for printing in order to produce a natural 3D construct that resembled bone tissue. The physical and biological features of the printed structures were evaluated entirely in vitro. Finally, a rat model was employed to examine the optimal 3D printed scaffold (5% ECM) as a bone transplant for the healing of cranial bone lesions. The present investigation demonstrated that decellularizing placental tissue fragments led to efficient removal of cell debris. In addition, a remarkable improvement in the printed scaffolds' mechanical and biological properties was observed by increasing the ECM concentration. The histology studies and real‐time PCR results demonstrated the acceleration of bone regeneration in the bone lesions treated with 5%ECM‐SF/Alg at 4 and 8 weeks after implantation. Overall, these results proved that the placental ECM‐printed scaffolds could potentially construct biomimetic grafts to reconstruct significant bone defects and now promise to proceed with clinical studies.https://doi.org/10.1002/elsc.2024000853D printed scaffoldalginate/silk fibroinhuman placenta |
| spellingShingle | Zahra Bashiri Zahra Khosrowpour Ali Moghaddaszadeh Davod Jafari Sanaz Alizadeh Hajar Nasiri Houman Parsaei Zahra Keshtkaran Meghdad Abdollahpour‐Alitappeh Farshad Bargrizaneh Behzad Rezaei Sara Simorgh Mazaher Gholipourmalekabadi Optimizations of Placenta Extracellular Matrix‐Loaded Silk Fibroin/Alginate 3D‐Printed Scaffolds Structurally and Functionally for Bone Tissue Engineering Engineering in Life Sciences 3D printed scaffold alginate/silk fibroin human placenta |
| title | Optimizations of Placenta Extracellular Matrix‐Loaded Silk Fibroin/Alginate 3D‐Printed Scaffolds Structurally and Functionally for Bone Tissue Engineering |
| title_full | Optimizations of Placenta Extracellular Matrix‐Loaded Silk Fibroin/Alginate 3D‐Printed Scaffolds Structurally and Functionally for Bone Tissue Engineering |
| title_fullStr | Optimizations of Placenta Extracellular Matrix‐Loaded Silk Fibroin/Alginate 3D‐Printed Scaffolds Structurally and Functionally for Bone Tissue Engineering |
| title_full_unstemmed | Optimizations of Placenta Extracellular Matrix‐Loaded Silk Fibroin/Alginate 3D‐Printed Scaffolds Structurally and Functionally for Bone Tissue Engineering |
| title_short | Optimizations of Placenta Extracellular Matrix‐Loaded Silk Fibroin/Alginate 3D‐Printed Scaffolds Structurally and Functionally for Bone Tissue Engineering |
| title_sort | optimizations of placenta extracellular matrix loaded silk fibroin alginate 3d printed scaffolds structurally and functionally for bone tissue engineering |
| topic | 3D printed scaffold alginate/silk fibroin human placenta |
| url | https://doi.org/10.1002/elsc.202400085 |
| work_keys_str_mv | AT zahrabashiri optimizationsofplacentaextracellularmatrixloadedsilkfibroinalginate3dprintedscaffoldsstructurallyandfunctionallyforbonetissueengineering AT zahrakhosrowpour optimizationsofplacentaextracellularmatrixloadedsilkfibroinalginate3dprintedscaffoldsstructurallyandfunctionallyforbonetissueengineering AT alimoghaddaszadeh optimizationsofplacentaextracellularmatrixloadedsilkfibroinalginate3dprintedscaffoldsstructurallyandfunctionallyforbonetissueengineering AT davodjafari optimizationsofplacentaextracellularmatrixloadedsilkfibroinalginate3dprintedscaffoldsstructurallyandfunctionallyforbonetissueengineering AT sanazalizadeh optimizationsofplacentaextracellularmatrixloadedsilkfibroinalginate3dprintedscaffoldsstructurallyandfunctionallyforbonetissueengineering AT hajarnasiri optimizationsofplacentaextracellularmatrixloadedsilkfibroinalginate3dprintedscaffoldsstructurallyandfunctionallyforbonetissueengineering AT houmanparsaei optimizationsofplacentaextracellularmatrixloadedsilkfibroinalginate3dprintedscaffoldsstructurallyandfunctionallyforbonetissueengineering AT zahrakeshtkaran optimizationsofplacentaextracellularmatrixloadedsilkfibroinalginate3dprintedscaffoldsstructurallyandfunctionallyforbonetissueengineering AT meghdadabdollahpouralitappeh optimizationsofplacentaextracellularmatrixloadedsilkfibroinalginate3dprintedscaffoldsstructurallyandfunctionallyforbonetissueengineering AT farshadbargrizaneh optimizationsofplacentaextracellularmatrixloadedsilkfibroinalginate3dprintedscaffoldsstructurallyandfunctionallyforbonetissueengineering AT behzadrezaei optimizationsofplacentaextracellularmatrixloadedsilkfibroinalginate3dprintedscaffoldsstructurallyandfunctionallyforbonetissueengineering AT sarasimorgh optimizationsofplacentaextracellularmatrixloadedsilkfibroinalginate3dprintedscaffoldsstructurallyandfunctionallyforbonetissueengineering AT mazahergholipourmalekabadi optimizationsofplacentaextracellularmatrixloadedsilkfibroinalginate3dprintedscaffoldsstructurallyandfunctionallyforbonetissueengineering |