Three Types of Broadly Reacting Antibodies against Influenza B Viruses Induced by Vaccination with Seasonal Influenza Viruses
We analyzed the antibody (Ab) repertoire against influenza B viruses induced by vaccination with seasonal influenza viruses in one individual who had never been vaccinated until 2009. The vaccine used in this study comprised B/Massachusetts/2/2012 (Yamagata lineage), A/Texas/50/2012 (H3N2), and A/Ca...
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2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/7251793 |
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| author | Daisuke Hirano Nobuko Ohshima Ritsuko Kubota-Koketsu Ayami Yamasaki Gene Kurosawa Yoshinobu Okuno Shunji Yoshida Yoshikazu Kurosawa |
| author_facet | Daisuke Hirano Nobuko Ohshima Ritsuko Kubota-Koketsu Ayami Yamasaki Gene Kurosawa Yoshinobu Okuno Shunji Yoshida Yoshikazu Kurosawa |
| author_sort | Daisuke Hirano |
| collection | DOAJ |
| description | We analyzed the antibody (Ab) repertoire against influenza B viruses induced by vaccination with seasonal influenza viruses in one individual who had never been vaccinated until 2009. The vaccine used in this study comprised B/Massachusetts/2/2012 (Yamagata lineage), A/Texas/50/2012 (H3N2), and A/California/7/2009 (H1N1). One month after the subject received two vaccinations, blood (200 ml) was obtained and peripheral mononuclear cells were prepared, and a large Ab library was constructed using phage display technology. The library was screened with HA-enriched fraction of B/Massachusetts/2/2012 and B/Brisbane/60/2008 (Victoria lineage) virus, and a total of 26 Abs that potentially bound to hemagglutinin (HA) molecules were isolated. Their binding activities to six influenza B viruses, three of Yamagata lineage and three of Victoria lineage, and two influenza A viruses, H1N1 and H3N2, were examined. The Abs showed cross-reactivity at three different levels. The first type bound to all Yamagata lineage viruses. The second type bound to both Yamagata and Victoria lineage viruses. The third type bound to both influenza A and B viruses. These results indicate that common epitopes exist on HA molecules of influenza virus at various levels, and humans have capability to produce Abs that bind to such common epitopes. |
| format | Article |
| id | doaj-art-cab4209450984c759f6f7b33c6ca3983 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-cab4209450984c759f6f7b33c6ca39832025-02-03T06:07:47ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/72517937251793Three Types of Broadly Reacting Antibodies against Influenza B Viruses Induced by Vaccination with Seasonal Influenza VirusesDaisuke Hirano0Nobuko Ohshima1Ritsuko Kubota-Koketsu2Ayami Yamasaki3Gene Kurosawa4Yoshinobu Okuno5Shunji Yoshida6Yoshikazu Kurosawa7Section of Rheumatology and Infectious Diseases, Department of Internal Medicine, Fujita Health University, Toyoake, Aichi 470-1192, JapanCenter for Collaboration in Research and Education, Fujita Health University, Toyoake, Aichi 470-1192, JapanClinical Research Section, Research Department, The Research Foundation for Microbial Disease of Osaka University, Suita, Osaka 565-0871, JapanDepartment of Innovation for Advanced Medicine, Center for Research Promotion and Support, Fujita Health University, Toyoake, Aichi 470-1192, JapanDepartment of Academic Research Support Promotion Facility, Center for Research Promotion and Support, Fujita Health University, Toyoake, Aichi 470-1192, JapanOsaka Institute of Public Health, Osaka, Osaka 537-0025, JapanSection of Rheumatology and Infectious Diseases, Department of Internal Medicine, Fujita Health University, Toyoake, Aichi 470-1192, JapanDepartment of Innovation for Advanced Medicine, Center for Research Promotion and Support, Fujita Health University, Toyoake, Aichi 470-1192, JapanWe analyzed the antibody (Ab) repertoire against influenza B viruses induced by vaccination with seasonal influenza viruses in one individual who had never been vaccinated until 2009. The vaccine used in this study comprised B/Massachusetts/2/2012 (Yamagata lineage), A/Texas/50/2012 (H3N2), and A/California/7/2009 (H1N1). One month after the subject received two vaccinations, blood (200 ml) was obtained and peripheral mononuclear cells were prepared, and a large Ab library was constructed using phage display technology. The library was screened with HA-enriched fraction of B/Massachusetts/2/2012 and B/Brisbane/60/2008 (Victoria lineage) virus, and a total of 26 Abs that potentially bound to hemagglutinin (HA) molecules were isolated. Their binding activities to six influenza B viruses, three of Yamagata lineage and three of Victoria lineage, and two influenza A viruses, H1N1 and H3N2, were examined. The Abs showed cross-reactivity at three different levels. The first type bound to all Yamagata lineage viruses. The second type bound to both Yamagata and Victoria lineage viruses. The third type bound to both influenza A and B viruses. These results indicate that common epitopes exist on HA molecules of influenza virus at various levels, and humans have capability to produce Abs that bind to such common epitopes.http://dx.doi.org/10.1155/2018/7251793 |
| spellingShingle | Daisuke Hirano Nobuko Ohshima Ritsuko Kubota-Koketsu Ayami Yamasaki Gene Kurosawa Yoshinobu Okuno Shunji Yoshida Yoshikazu Kurosawa Three Types of Broadly Reacting Antibodies against Influenza B Viruses Induced by Vaccination with Seasonal Influenza Viruses Journal of Immunology Research |
| title | Three Types of Broadly Reacting Antibodies against Influenza B Viruses Induced by Vaccination with Seasonal Influenza Viruses |
| title_full | Three Types of Broadly Reacting Antibodies against Influenza B Viruses Induced by Vaccination with Seasonal Influenza Viruses |
| title_fullStr | Three Types of Broadly Reacting Antibodies against Influenza B Viruses Induced by Vaccination with Seasonal Influenza Viruses |
| title_full_unstemmed | Three Types of Broadly Reacting Antibodies against Influenza B Viruses Induced by Vaccination with Seasonal Influenza Viruses |
| title_short | Three Types of Broadly Reacting Antibodies against Influenza B Viruses Induced by Vaccination with Seasonal Influenza Viruses |
| title_sort | three types of broadly reacting antibodies against influenza b viruses induced by vaccination with seasonal influenza viruses |
| url | http://dx.doi.org/10.1155/2018/7251793 |
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