Elevated Matrix Metalloproteinase Levels in Bronchi Infected with Periodontopathogenic Bacteria.
<h4>Objectives</h4>To determine whether bronchial colonisations/infections with periodontopathogenic bacteria are associated with elevated inflammatory markers such as MMPs, interleukins and Tumor necrosis factor alpha in the bronchial fluid.<h4>Methods</h4>Periodontal status...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0144461&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849722886875512832 |
|---|---|
| author | Luca Bernasconi Liza L Ramenzoni Ahmed Al-Majid Gabrielo M Tini Sereina M Graber Patrick R Schmidlin Sarosh Irani |
| author_facet | Luca Bernasconi Liza L Ramenzoni Ahmed Al-Majid Gabrielo M Tini Sereina M Graber Patrick R Schmidlin Sarosh Irani |
| author_sort | Luca Bernasconi |
| collection | DOAJ |
| description | <h4>Objectives</h4>To determine whether bronchial colonisations/infections with periodontopathogenic bacteria are associated with elevated inflammatory markers such as MMPs, interleukins and Tumor necrosis factor alpha in the bronchial fluid.<h4>Methods</h4>Periodontal status was assessed in consecutive outpatients planned for elective bronchoscopies, and PCR for periodontopathogenic bacteria was performed from a protected specimen brush sample taken from the bronchial mucosa. Additionally, MMPs, interleukins and Tumor necrosis factor alpha were measured in the bronchial fluid.<h4>Results</h4>Out of the four species assessed, one species was found in 13 of 91 (14%) patients, and two in 12 (13%), three in 13 (14%) and all four in 1 (1%) patient, respectively. In multiple linear regression models the presence of Treponema denticola showed a consistent pattern of positive effects in bronchial fluid (Bonferroni adjusted p-values) on the levels of MMP9 (p adj.: 0.028) and MMP12 (p adj.: 0.029). Active smoking was independently associated with increased levels of aMMP8 (p adj.: 0.005) and MMP9 (p adj.: 0.009). Levels of IL-1 ß, IL-8 and Tumor necrosis factor alpha measured in the bronchial fluid were not affected by the presence of periodontopathogenic bacteria.<h4>Conclusions</h4>Bronchial colonisation/infection with Treponema denticola and smoking are independently associated with elevated MMPs (MMP9/MMP12 and MMP8/MMP9, respectively) in the bronchial fluid. |
| format | Article |
| id | doaj-art-ca9155e960cb4da0b282dbcf8e95b2f4 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-ca9155e960cb4da0b282dbcf8e95b2f42025-08-20T03:11:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011012e014446110.1371/journal.pone.0144461Elevated Matrix Metalloproteinase Levels in Bronchi Infected with Periodontopathogenic Bacteria.Luca BernasconiLiza L RamenzoniAhmed Al-MajidGabrielo M TiniSereina M GraberPatrick R SchmidlinSarosh Irani<h4>Objectives</h4>To determine whether bronchial colonisations/infections with periodontopathogenic bacteria are associated with elevated inflammatory markers such as MMPs, interleukins and Tumor necrosis factor alpha in the bronchial fluid.<h4>Methods</h4>Periodontal status was assessed in consecutive outpatients planned for elective bronchoscopies, and PCR for periodontopathogenic bacteria was performed from a protected specimen brush sample taken from the bronchial mucosa. Additionally, MMPs, interleukins and Tumor necrosis factor alpha were measured in the bronchial fluid.<h4>Results</h4>Out of the four species assessed, one species was found in 13 of 91 (14%) patients, and two in 12 (13%), three in 13 (14%) and all four in 1 (1%) patient, respectively. In multiple linear regression models the presence of Treponema denticola showed a consistent pattern of positive effects in bronchial fluid (Bonferroni adjusted p-values) on the levels of MMP9 (p adj.: 0.028) and MMP12 (p adj.: 0.029). Active smoking was independently associated with increased levels of aMMP8 (p adj.: 0.005) and MMP9 (p adj.: 0.009). Levels of IL-1 ß, IL-8 and Tumor necrosis factor alpha measured in the bronchial fluid were not affected by the presence of periodontopathogenic bacteria.<h4>Conclusions</h4>Bronchial colonisation/infection with Treponema denticola and smoking are independently associated with elevated MMPs (MMP9/MMP12 and MMP8/MMP9, respectively) in the bronchial fluid.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0144461&type=printable |
| spellingShingle | Luca Bernasconi Liza L Ramenzoni Ahmed Al-Majid Gabrielo M Tini Sereina M Graber Patrick R Schmidlin Sarosh Irani Elevated Matrix Metalloproteinase Levels in Bronchi Infected with Periodontopathogenic Bacteria. PLoS ONE |
| title | Elevated Matrix Metalloproteinase Levels in Bronchi Infected with Periodontopathogenic Bacteria. |
| title_full | Elevated Matrix Metalloproteinase Levels in Bronchi Infected with Periodontopathogenic Bacteria. |
| title_fullStr | Elevated Matrix Metalloproteinase Levels in Bronchi Infected with Periodontopathogenic Bacteria. |
| title_full_unstemmed | Elevated Matrix Metalloproteinase Levels in Bronchi Infected with Periodontopathogenic Bacteria. |
| title_short | Elevated Matrix Metalloproteinase Levels in Bronchi Infected with Periodontopathogenic Bacteria. |
| title_sort | elevated matrix metalloproteinase levels in bronchi infected with periodontopathogenic bacteria |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0144461&type=printable |
| work_keys_str_mv | AT lucabernasconi elevatedmatrixmetalloproteinaselevelsinbronchiinfectedwithperiodontopathogenicbacteria AT lizalramenzoni elevatedmatrixmetalloproteinaselevelsinbronchiinfectedwithperiodontopathogenicbacteria AT ahmedalmajid elevatedmatrixmetalloproteinaselevelsinbronchiinfectedwithperiodontopathogenicbacteria AT gabrielomtini elevatedmatrixmetalloproteinaselevelsinbronchiinfectedwithperiodontopathogenicbacteria AT sereinamgraber elevatedmatrixmetalloproteinaselevelsinbronchiinfectedwithperiodontopathogenicbacteria AT patrickrschmidlin elevatedmatrixmetalloproteinaselevelsinbronchiinfectedwithperiodontopathogenicbacteria AT saroshirani elevatedmatrixmetalloproteinaselevelsinbronchiinfectedwithperiodontopathogenicbacteria |