Evaluation of Carboxymethyl Cellulose as an Additive for Selective Protein Removal from Wine

Achieving protein stability is one of the main objectives before bottling wine. Traditionally, this is accomplished via bentonite fining, but the application has drawbacks and is not the most sustainable practice. A promising alternative was previously identified in modified cellulose, which is appr...

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Main Author: Stephan Sommer
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Fermentation
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Online Access:https://www.mdpi.com/2311-5637/11/5/273
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author Stephan Sommer
author_facet Stephan Sommer
author_sort Stephan Sommer
collection DOAJ
description Achieving protein stability is one of the main objectives before bottling wine. Traditionally, this is accomplished via bentonite fining, but the application has drawbacks and is not the most sustainable practice. A promising alternative was previously identified in modified cellulose, which is approved for tartrate stabilization but, as a side activity, could also help remove protein from wine. This study was designed to evaluate powdered carboxymethyl cellulose (CMC) and a liquid formulation in model wine using bovine serum albumin (BSA) and egg white as model proteins. The solubility of BSA proved to be insufficient, so experiments in wine were conducted with egg white protein only. Low-addition levels of liquid CMC showed the highest protein removal rates in real wine, so final trials were conducted with 13 commercial wines to evaluate the performance in different wine styles. The protein removal rate ranged from 12% to 84%, with an overall average of 57%. While these results do not reach the efficiency of bentonite, CMC is showing promise as an additional stabilization tool for a wide variety of wines. It can stabilize over the entire pH range of wine between 2.9 and 4.1, which is a unique feature of this method.
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spelling doaj-art-ca7ee28fe1bc4a1fbb686e6f2cccae7b2025-08-20T02:33:48ZengMDPI AGFermentation2311-56372025-05-0111527310.3390/fermentation11050273Evaluation of Carboxymethyl Cellulose as an Additive for Selective Protein Removal from WineStephan Sommer0Grape and Wine Institute, University of Missouri-Columbia, 223 Eckles Hall, Columbia, MO 65211, USAAchieving protein stability is one of the main objectives before bottling wine. Traditionally, this is accomplished via bentonite fining, but the application has drawbacks and is not the most sustainable practice. A promising alternative was previously identified in modified cellulose, which is approved for tartrate stabilization but, as a side activity, could also help remove protein from wine. This study was designed to evaluate powdered carboxymethyl cellulose (CMC) and a liquid formulation in model wine using bovine serum albumin (BSA) and egg white as model proteins. The solubility of BSA proved to be insufficient, so experiments in wine were conducted with egg white protein only. Low-addition levels of liquid CMC showed the highest protein removal rates in real wine, so final trials were conducted with 13 commercial wines to evaluate the performance in different wine styles. The protein removal rate ranged from 12% to 84%, with an overall average of 57%. While these results do not reach the efficiency of bentonite, CMC is showing promise as an additional stabilization tool for a wide variety of wines. It can stabilize over the entire pH range of wine between 2.9 and 4.1, which is a unique feature of this method.https://www.mdpi.com/2311-5637/11/5/273bovine serum albuminegg whitezeta potentialprecipitationstability
spellingShingle Stephan Sommer
Evaluation of Carboxymethyl Cellulose as an Additive for Selective Protein Removal from Wine
Fermentation
bovine serum albumin
egg white
zeta potential
precipitation
stability
title Evaluation of Carboxymethyl Cellulose as an Additive for Selective Protein Removal from Wine
title_full Evaluation of Carboxymethyl Cellulose as an Additive for Selective Protein Removal from Wine
title_fullStr Evaluation of Carboxymethyl Cellulose as an Additive for Selective Protein Removal from Wine
title_full_unstemmed Evaluation of Carboxymethyl Cellulose as an Additive for Selective Protein Removal from Wine
title_short Evaluation of Carboxymethyl Cellulose as an Additive for Selective Protein Removal from Wine
title_sort evaluation of carboxymethyl cellulose as an additive for selective protein removal from wine
topic bovine serum albumin
egg white
zeta potential
precipitation
stability
url https://www.mdpi.com/2311-5637/11/5/273
work_keys_str_mv AT stephansommer evaluationofcarboxymethylcelluloseasanadditiveforselectiveproteinremovalfromwine