LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts
The ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested. The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method. Fractions of free and conjugated phytosterols were isol...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/526818 |
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| _version_ | 1832554179310125056 |
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| author | Štěpán Horník Marie Sajfrtová Jindřich Karban Jan Sýkora Anna Březinová Zdeněk Wimmer |
| author_facet | Štěpán Horník Marie Sajfrtová Jindřich Karban Jan Sýkora Anna Březinová Zdeněk Wimmer |
| author_sort | Štěpán Horník |
| collection | DOAJ |
| description | The ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested. The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method. Fractions of free and conjugated phytosterols were isolated and analyzed by isocratic HPLC-NMR methods. The results of qualitative and quantitative analyses were in a good agreement with the literature data. |
| format | Article |
| id | doaj-art-ca6a34d3644f4836a87938363409f0f4 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-ca6a34d3644f4836a87938363409f0f42025-02-03T05:52:15ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/526818526818LC-NMR Technique in the Analysis of Phytosterols in Natural ExtractsŠtěpán Horník0Marie Sajfrtová1Jindřich Karban2Jan Sýkora3Anna Březinová4Zdeněk Wimmer5Institute of Chemical Process Fundamentals of the ASCR, v.v.i., Rozvojová 2/135, 16502 Prague 6, Czech RepublicInstitute of Chemical Process Fundamentals of the ASCR, v.v.i., Rozvojová 2/135, 16502 Prague 6, Czech RepublicInstitute of Chemical Process Fundamentals of the ASCR, v.v.i., Rozvojová 2/135, 16502 Prague 6, Czech RepublicInstitute of Chemical Process Fundamentals of the ASCR, v.v.i., Rozvojová 2/135, 16502 Prague 6, Czech RepublicInstitute of Organic Chemistry and Biochemistry of the ASCR, v.v.i., Flemingovo Náměstí 2, 16610 Prague 6, Czech RepublicInstitute of Experimental Botany of the ASCR, v.v.i., Isotope Laboratory, Vídeňská 1083, 14220 Prague 4, Czech RepublicThe ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested. The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method. Fractions of free and conjugated phytosterols were isolated and analyzed by isocratic HPLC-NMR methods. The results of qualitative and quantitative analyses were in a good agreement with the literature data.http://dx.doi.org/10.1155/2013/526818 |
| spellingShingle | Štěpán Horník Marie Sajfrtová Jindřich Karban Jan Sýkora Anna Březinová Zdeněk Wimmer LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts Journal of Analytical Methods in Chemistry |
| title | LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts |
| title_full | LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts |
| title_fullStr | LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts |
| title_full_unstemmed | LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts |
| title_short | LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts |
| title_sort | lc nmr technique in the analysis of phytosterols in natural extracts |
| url | http://dx.doi.org/10.1155/2013/526818 |
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