High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland
Mastitis is an economically important disease in which fungi belonging to the genus Candida may participate as etiological agents. This study focused on determining the frequency of fungal isolation and differentiation of fungal species isolated from milk of mastitic cows. Sixty-six milk samples fro...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/196347 |
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| author | Bozena Dworecka-Kaszak Alicja Krutkiewicz Daniel Szopa Miroslaw Kleczkowski Malgorzata Biegańska |
| author_facet | Bozena Dworecka-Kaszak Alicja Krutkiewicz Daniel Szopa Miroslaw Kleczkowski Malgorzata Biegańska |
| author_sort | Bozena Dworecka-Kaszak |
| collection | DOAJ |
| description | Mastitis is an economically important disease in which fungi belonging to the genus Candida may participate as etiological agents. This study focused on determining the frequency of fungal isolation and differentiation of fungal species isolated from milk of mastitic cows. Sixty-six milk samples from mastitic cows were studied, and 55 strains of fungi were isolated. Seven different species classified as Candida were identified basing on phenotypic properties, and the dominating species was C. parapsilosis. Genomic DNA was isolated and amplified in PCR with ITS1 and NL2 primers. Amplification products were digested with restriction enzymes HpaII and EcoRI. Amplification of DNA with ITS1 and NL2 primers resulted in products of different sizes. Comparison of product sizes in restriction fragment PCR REA confirmed differences among species. Strains grouped together on the basis of phenotype characteristics differed in restriction fragment profiles. None of the investigated species showed similar genetic profiles. |
| format | Article |
| id | doaj-art-ca59b24c929c4303a24c85248781658c |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-ca59b24c929c4303a24c85248781658c2025-02-03T01:28:05ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/196347196347High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in PolandBozena Dworecka-Kaszak0Alicja Krutkiewicz1Daniel Szopa2Miroslaw Kleczkowski3Malgorzata Biegańska4Mycology Division, Preclinical Science Department, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-789 Warsaw, PolandMycology Division, Preclinical Science Department, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-789 Warsaw, PolandMolecular Genetic Division, Mother and Child institute, Kasprzaka 17a, 01-211 Warsaw, PolandDivision of Laboratory Diagnostics, Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, PolandMycology Division, Preclinical Science Department, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-789 Warsaw, PolandMastitis is an economically important disease in which fungi belonging to the genus Candida may participate as etiological agents. This study focused on determining the frequency of fungal isolation and differentiation of fungal species isolated from milk of mastitic cows. Sixty-six milk samples from mastitic cows were studied, and 55 strains of fungi were isolated. Seven different species classified as Candida were identified basing on phenotypic properties, and the dominating species was C. parapsilosis. Genomic DNA was isolated and amplified in PCR with ITS1 and NL2 primers. Amplification products were digested with restriction enzymes HpaII and EcoRI. Amplification of DNA with ITS1 and NL2 primers resulted in products of different sizes. Comparison of product sizes in restriction fragment PCR REA confirmed differences among species. Strains grouped together on the basis of phenotype characteristics differed in restriction fragment profiles. None of the investigated species showed similar genetic profiles.http://dx.doi.org/10.1100/2012/196347 |
| spellingShingle | Bozena Dworecka-Kaszak Alicja Krutkiewicz Daniel Szopa Miroslaw Kleczkowski Malgorzata Biegańska High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland The Scientific World Journal |
| title | High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland |
| title_full | High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland |
| title_fullStr | High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland |
| title_full_unstemmed | High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland |
| title_short | High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland |
| title_sort | high prevalence of candida yeast in milk samples from cows suffering from mastitis in poland |
| url | http://dx.doi.org/10.1100/2012/196347 |
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