Matrine Attenuates COX-2 and ICAM-1 Expressions in Human Lung Epithelial Cells and Prevents Acute Lung Injury in LPS-Induced Mice
Matrine is isolated from Sophora flavescens and shows anti-inflammatory effects in macrophages. Here we evaluated matrine’s suppressive effects on cyclooxygenase 2 (COX-2) and intercellular adhesion molecule-1 (ICAM-1) expressions in lipopolysaccharide- (LPS-) stimulated human lung epithelial A549 c...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/3630485 |
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| author | Chian-Jiun Liou You-Rong Lai Ya-Ling Chen Yi-Hsien Chang Zih-Ying Li Wen-Chung Huang |
| author_facet | Chian-Jiun Liou You-Rong Lai Ya-Ling Chen Yi-Hsien Chang Zih-Ying Li Wen-Chung Huang |
| author_sort | Chian-Jiun Liou |
| collection | DOAJ |
| description | Matrine is isolated from Sophora flavescens and shows anti-inflammatory effects in macrophages. Here we evaluated matrine’s suppressive effects on cyclooxygenase 2 (COX-2) and intercellular adhesion molecule-1 (ICAM-1) expressions in lipopolysaccharide- (LPS-) stimulated human lung epithelial A549 cells. Additionally, BALB/c mice were given various matrine doses by intraperitoneal injection, and then lung injury was induced via intratracheal instillation of LPS. In LPS-stimulated A549 cells, matrine inhibited the productions of interleukin-8 (IL-8), monocyte chemotactic protein-1, and IL-6 and decreased COX-2 expression. Matrine treatment also decreased ICAM-1 protein expression and suppressed the adhesion of neutrophil-like cells to inflammatory A549 cells. In vitro results demonstrated that matrine significantly inhibited mitogen-activated protein kinase phosphorylation and decreased nuclear transcription factor kappa-B subunit p65 protein translocation into the nucleus. In vivo data indicated that matrine significantly inhibited neutrophil infiltration and suppressed productions of tumor necrosis factor-α and IL-6 in mouse bronchoalveolar lavage fluid and serum. Analysis of lung tissue showed that matrine decreased the gene expression of proinflammatory cytokines, chemokines, COX-2, and ICAM-1. Our findings suggest that matrine improved lung injury in mice and decreased the inflammatory response in human lung epithelial cells. |
| format | Article |
| id | doaj-art-ca42501adb74442086f1283cb62b0666 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-ca42501adb74442086f1283cb62b06662025-02-03T05:51:26ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/36304853630485Matrine Attenuates COX-2 and ICAM-1 Expressions in Human Lung Epithelial Cells and Prevents Acute Lung Injury in LPS-Induced MiceChian-Jiun Liou0You-Rong Lai1Ya-Ling Chen2Yi-Hsien Chang3Zih-Ying Li4Wen-Chung Huang5Department of Nursing, Chang Gung University of Science and Technology, No. 261 Wenhua 1st Road, Guishan District, Taoyuan City 33303, TaiwanDepartment of Nutrition and Health Sciences, Chang Gung University of Science and Technology, No. 261 Wenhua 1st Road, Guishan District, Taoyuan City 33303, TaiwanDepartment of Nutrition and Health Sciences, Chang Gung University of Science and Technology, No. 261 Wenhua 1st Road, Guishan District, Taoyuan City 33303, TaiwanDepartment of Nutrition and Health Sciences, Chang Gung University of Science and Technology, No. 261 Wenhua 1st Road, Guishan District, Taoyuan City 33303, TaiwanChang Gung Memorial Hospital at Linkou, Guishan District, Taoyuan City 33303, TaiwanChang Gung Memorial Hospital at Linkou, Guishan District, Taoyuan City 33303, TaiwanMatrine is isolated from Sophora flavescens and shows anti-inflammatory effects in macrophages. Here we evaluated matrine’s suppressive effects on cyclooxygenase 2 (COX-2) and intercellular adhesion molecule-1 (ICAM-1) expressions in lipopolysaccharide- (LPS-) stimulated human lung epithelial A549 cells. Additionally, BALB/c mice were given various matrine doses by intraperitoneal injection, and then lung injury was induced via intratracheal instillation of LPS. In LPS-stimulated A549 cells, matrine inhibited the productions of interleukin-8 (IL-8), monocyte chemotactic protein-1, and IL-6 and decreased COX-2 expression. Matrine treatment also decreased ICAM-1 protein expression and suppressed the adhesion of neutrophil-like cells to inflammatory A549 cells. In vitro results demonstrated that matrine significantly inhibited mitogen-activated protein kinase phosphorylation and decreased nuclear transcription factor kappa-B subunit p65 protein translocation into the nucleus. In vivo data indicated that matrine significantly inhibited neutrophil infiltration and suppressed productions of tumor necrosis factor-α and IL-6 in mouse bronchoalveolar lavage fluid and serum. Analysis of lung tissue showed that matrine decreased the gene expression of proinflammatory cytokines, chemokines, COX-2, and ICAM-1. Our findings suggest that matrine improved lung injury in mice and decreased the inflammatory response in human lung epithelial cells.http://dx.doi.org/10.1155/2016/3630485 |
| spellingShingle | Chian-Jiun Liou You-Rong Lai Ya-Ling Chen Yi-Hsien Chang Zih-Ying Li Wen-Chung Huang Matrine Attenuates COX-2 and ICAM-1 Expressions in Human Lung Epithelial Cells and Prevents Acute Lung Injury in LPS-Induced Mice Mediators of Inflammation |
| title | Matrine Attenuates COX-2 and ICAM-1 Expressions in Human Lung Epithelial Cells and Prevents Acute Lung Injury in LPS-Induced Mice |
| title_full | Matrine Attenuates COX-2 and ICAM-1 Expressions in Human Lung Epithelial Cells and Prevents Acute Lung Injury in LPS-Induced Mice |
| title_fullStr | Matrine Attenuates COX-2 and ICAM-1 Expressions in Human Lung Epithelial Cells and Prevents Acute Lung Injury in LPS-Induced Mice |
| title_full_unstemmed | Matrine Attenuates COX-2 and ICAM-1 Expressions in Human Lung Epithelial Cells and Prevents Acute Lung Injury in LPS-Induced Mice |
| title_short | Matrine Attenuates COX-2 and ICAM-1 Expressions in Human Lung Epithelial Cells and Prevents Acute Lung Injury in LPS-Induced Mice |
| title_sort | matrine attenuates cox 2 and icam 1 expressions in human lung epithelial cells and prevents acute lung injury in lps induced mice |
| url | http://dx.doi.org/10.1155/2016/3630485 |
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