Elucidating Antibacterial Activity and Mechanism of Daphnetin against Pseudomonas fluorescens and Shewanella putrefaciens
In this research, the antibacterial activity and mechanism of daphnetin against Pseudomonas fluorescens and Shewanella putrefaciens were evaluated. The minimum inhibitory concentration (MIC) of daphnetin on P. fluorescens and S. putrefaciens was 0.16 and 0.08 mg·mL−1, respectively. The growth curve...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2020/6622355 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832565675880611840 |
|---|---|
| author | Wenru Liu Jun Mei Jing Xie |
| author_facet | Wenru Liu Jun Mei Jing Xie |
| author_sort | Wenru Liu |
| collection | DOAJ |
| description | In this research, the antibacterial activity and mechanism of daphnetin against Pseudomonas fluorescens and Shewanella putrefaciens were evaluated. The minimum inhibitory concentration (MIC) of daphnetin on P. fluorescens and S. putrefaciens was 0.16 and 0.08 mg·mL−1, respectively. The growth curve test also showed that daphnetin had a good antibacterial effect. The results of intracellular component leakage and cell viability analysis illustrated that daphnetin destroyed the morphology of the cell membrane. According to scanning electron microscope and transmission electron microscope observations, the treated bacterial cells displayed obvious morphological and ultrastructural changes in the cell membrane of the two tested strains, whichconfirmed daphnetin’s damage to the integrity of the cell membrane. The findings indicated that daphnetin mainly exerted its antibacterial effect by destroying the membrane and suggested that it had good potential to be as a natural food preservative. |
| format | Article |
| id | doaj-art-ca3e7bf95c404f2d95b9e27df24a27d2 |
| institution | Kabale University |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-ca3e7bf95c404f2d95b9e27df24a27d22025-02-03T01:07:08ZengWileyJournal of Food Quality0146-94281745-45572020-01-01202010.1155/2020/66223556622355Elucidating Antibacterial Activity and Mechanism of Daphnetin against Pseudomonas fluorescens and Shewanella putrefaciensWenru Liu0Jun Mei1Jing Xie2College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaIn this research, the antibacterial activity and mechanism of daphnetin against Pseudomonas fluorescens and Shewanella putrefaciens were evaluated. The minimum inhibitory concentration (MIC) of daphnetin on P. fluorescens and S. putrefaciens was 0.16 and 0.08 mg·mL−1, respectively. The growth curve test also showed that daphnetin had a good antibacterial effect. The results of intracellular component leakage and cell viability analysis illustrated that daphnetin destroyed the morphology of the cell membrane. According to scanning electron microscope and transmission electron microscope observations, the treated bacterial cells displayed obvious morphological and ultrastructural changes in the cell membrane of the two tested strains, whichconfirmed daphnetin’s damage to the integrity of the cell membrane. The findings indicated that daphnetin mainly exerted its antibacterial effect by destroying the membrane and suggested that it had good potential to be as a natural food preservative.http://dx.doi.org/10.1155/2020/6622355 |
| spellingShingle | Wenru Liu Jun Mei Jing Xie Elucidating Antibacterial Activity and Mechanism of Daphnetin against Pseudomonas fluorescens and Shewanella putrefaciens Journal of Food Quality |
| title | Elucidating Antibacterial Activity and Mechanism of Daphnetin against Pseudomonas fluorescens and Shewanella putrefaciens |
| title_full | Elucidating Antibacterial Activity and Mechanism of Daphnetin against Pseudomonas fluorescens and Shewanella putrefaciens |
| title_fullStr | Elucidating Antibacterial Activity and Mechanism of Daphnetin against Pseudomonas fluorescens and Shewanella putrefaciens |
| title_full_unstemmed | Elucidating Antibacterial Activity and Mechanism of Daphnetin against Pseudomonas fluorescens and Shewanella putrefaciens |
| title_short | Elucidating Antibacterial Activity and Mechanism of Daphnetin against Pseudomonas fluorescens and Shewanella putrefaciens |
| title_sort | elucidating antibacterial activity and mechanism of daphnetin against pseudomonas fluorescens and shewanella putrefaciens |
| url | http://dx.doi.org/10.1155/2020/6622355 |
| work_keys_str_mv | AT wenruliu elucidatingantibacterialactivityandmechanismofdaphnetinagainstpseudomonasfluorescensandshewanellaputrefaciens AT junmei elucidatingantibacterialactivityandmechanismofdaphnetinagainstpseudomonasfluorescensandshewanellaputrefaciens AT jingxie elucidatingantibacterialactivityandmechanismofdaphnetinagainstpseudomonasfluorescensandshewanellaputrefaciens |