The Role of Fas-FasL Signaling Pathway in Induction of Apoptosis in Patients with Sulfur Mustard-Induced Chronic Bronchiolitis
Sulfur mustard (SM) is an alkylating agent that induces apoptosis and necrosis in cells. Fas-Fas ligand (FasL) interaction could induce apoptosis as well. In this study, it was hypothesized that apoptosis might play an important role in the pathogenesis of SM-induced lung injury via Fas-FasL signali...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2010/373612 |
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| author | Gila Pirzad Mahvash Jafari Sasan Tavana Homayoon Sadrayee Saeid Ghavami Arezoo Shajiei Mostafa Ghanei |
| author_facet | Gila Pirzad Mahvash Jafari Sasan Tavana Homayoon Sadrayee Saeid Ghavami Arezoo Shajiei Mostafa Ghanei |
| author_sort | Gila Pirzad |
| collection | DOAJ |
| description | Sulfur mustard (SM) is an alkylating agent that induces apoptosis and necrosis in cells. Fas-Fas ligand (FasL) interaction could induce apoptosis as well. In this study, it was hypothesized that apoptosis might play an important role in the pathogenesis of SM-induced lung injury via Fas-FasL signaling pathway. In a case-control study, Fas and FasL levels, caspase-3 activity and percent of apoptotic cells were measured in bronchoalveolar lavage (BAL) fluid of patients 20 years after exposure to sulfur mustard and compared with the control group.
Results show that Fas and FasL levels were significantly higher in BAL fluid cells in patients group compared with the control (P=.001). No significant differences were observed between mild and moderate-severe groups. BAL fluid cells caspase-3 activity was not significantly different among the mild, moderate-severe, and control groups. The data suggest that Fas-FasL-induced apoptosis was impaired in BAL fluid cells of SM-exposed patients which might be one of the initiators of pathogenesis in SM-induced lung injury in these patients. |
| format | Article |
| id | doaj-art-ca3d2a8144554b83a4470a9bfa51ebb3 |
| institution | Kabale University |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-ca3d2a8144554b83a4470a9bfa51ebb32025-02-03T01:26:48ZengWileyJournal of Toxicology1687-81911687-82052010-01-01201010.1155/2010/373612373612The Role of Fas-FasL Signaling Pathway in Induction of Apoptosis in Patients with Sulfur Mustard-Induced Chronic BronchiolitisGila Pirzad0Mahvash Jafari1Sasan Tavana2Homayoon Sadrayee3Saeid Ghavami4Arezoo Shajiei5Mostafa Ghanei6Chemical Injury Research Center, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran 1956837173, IranDepartment of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran 1956837173, IranClinical Research and Development Center, Shahid Modarres Hospital, Shahid Beheshti University, Tehran 1991733981, IranDepartment of Anatomy, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran 14359151371, IranNational Training Program in Allergy and Asthma, University of Manitoba, Winnipeg, MB, R3T 2N2, CanadaBiotechnology Center, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran 1956837173, IranChemical Injury Research Center, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran 1956837173, IranSulfur mustard (SM) is an alkylating agent that induces apoptosis and necrosis in cells. Fas-Fas ligand (FasL) interaction could induce apoptosis as well. In this study, it was hypothesized that apoptosis might play an important role in the pathogenesis of SM-induced lung injury via Fas-FasL signaling pathway. In a case-control study, Fas and FasL levels, caspase-3 activity and percent of apoptotic cells were measured in bronchoalveolar lavage (BAL) fluid of patients 20 years after exposure to sulfur mustard and compared with the control group. Results show that Fas and FasL levels were significantly higher in BAL fluid cells in patients group compared with the control (P=.001). No significant differences were observed between mild and moderate-severe groups. BAL fluid cells caspase-3 activity was not significantly different among the mild, moderate-severe, and control groups. The data suggest that Fas-FasL-induced apoptosis was impaired in BAL fluid cells of SM-exposed patients which might be one of the initiators of pathogenesis in SM-induced lung injury in these patients.http://dx.doi.org/10.1155/2010/373612 |
| spellingShingle | Gila Pirzad Mahvash Jafari Sasan Tavana Homayoon Sadrayee Saeid Ghavami Arezoo Shajiei Mostafa Ghanei The Role of Fas-FasL Signaling Pathway in Induction of Apoptosis in Patients with Sulfur Mustard-Induced Chronic Bronchiolitis Journal of Toxicology |
| title | The Role of Fas-FasL Signaling Pathway in Induction of Apoptosis in Patients with Sulfur Mustard-Induced Chronic Bronchiolitis |
| title_full | The Role of Fas-FasL Signaling Pathway in Induction of Apoptosis in Patients with Sulfur Mustard-Induced Chronic Bronchiolitis |
| title_fullStr | The Role of Fas-FasL Signaling Pathway in Induction of Apoptosis in Patients with Sulfur Mustard-Induced Chronic Bronchiolitis |
| title_full_unstemmed | The Role of Fas-FasL Signaling Pathway in Induction of Apoptosis in Patients with Sulfur Mustard-Induced Chronic Bronchiolitis |
| title_short | The Role of Fas-FasL Signaling Pathway in Induction of Apoptosis in Patients with Sulfur Mustard-Induced Chronic Bronchiolitis |
| title_sort | role of fas fasl signaling pathway in induction of apoptosis in patients with sulfur mustard induced chronic bronchiolitis |
| url | http://dx.doi.org/10.1155/2010/373612 |
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