Microbial signatures in head and neck squamous cell carcinoma: an in silico study
Abstract Objectives The oral cavity harbors a plethora of bacterial species. Dysbiosis of oral and gut microbiota is associated with several oral and systemic pathologies, such as cancer, obesity, diabetes, atherosclerosis and gastrointestinal diseases. Imbalance in the oral-gut microbial axis has...
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| Format: | Article |
| Language: | English |
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University of São Paulo
2025-02-01
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| Series: | Journal of Applied Oral Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572025000100405&lng=en&tlng=en |
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| author | Loganathan KAVITHA Manogaran KUZHALMOZHI Jayaseelan VIJAYASHREE PRIYADHARSINI Arunachalam ARUN KUMAR Krishna Mohan Rao UMADEVI Kannan RANGANATHAN |
| author_facet | Loganathan KAVITHA Manogaran KUZHALMOZHI Jayaseelan VIJAYASHREE PRIYADHARSINI Arunachalam ARUN KUMAR Krishna Mohan Rao UMADEVI Kannan RANGANATHAN |
| author_sort | Loganathan KAVITHA |
| collection | DOAJ |
| description | Abstract Objectives The oral cavity harbors a plethora of bacterial species. Dysbiosis of oral and gut microbiota is associated with several oral and systemic pathologies, such as cancer, obesity, diabetes, atherosclerosis and gastrointestinal diseases. Imbalance in the oral-gut microbial axis has been associated with head and neck squamous cell carcinoma (HNSCC). This study aims to analyze the bacterial profile of HNSCC across various taxonomic units, investigate molecular patterns associated with prevalent bacterial phylum in HNSCC, and compare the bacterial profile in HNSCC and gastrointestinal (GI) carcinoma using computational analysis. Methodology The microbe-host transcriptomic, proteomic, and epigenetic analyses of HNSCC and GI carcinomas were performed using The Cancer Microbiome Atlas (TCMA) database. The differential expression of the host’s mRNA transcripts and proteins associated with tumor microbiome were analyzed using The University of Alabama at Birmingham Cancer data analysis (UALCAN) and Clinical Proteomic Tumor Analysis Consortium (CPTAC) websites. Results A decrease in Actinobacteria and an enrichment of Flavobacteria at the class level, Neisseriales, Pasteurellales, and Campylobacterales at the order level, Pasteurellaceae, Flavobacteriaceae, Campylobacteraceae, and Peptoniphilaceae at the family level, and Hemophilus, Porphyromonas, and Leptotrichia at the genus level were observed in HNSCC compared to the normal mucosa. RICTOR protein, mRNA transcripts (HIST1H2BB, SCARNA11, TBC1D21 gene), and hsa-miR-200a-5p miRNA were significantly correlated with prevalent bacterial species in HNSCC. A major increase in Actinobacteria, Fusobacteria, and Spirochaetes was observed in HNSCC compared to GI carcinoma. Conclusion The oral-gut microbial dysbiosis, as reflected by the differential abundance of bacterial species in oral and GI carcinomas, suggests the implication of tumor microbiome and their genomic interactions with the host in carcinogenesis. |
| format | Article |
| id | doaj-art-ca3923f74f4b4252b6f97c4e8d0e9d60 |
| institution | Kabale University |
| issn | 1678-7765 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj-art-ca3923f74f4b4252b6f97c4e8d0e9d602025-02-04T07:40:35ZengUniversity of São PauloJournal of Applied Oral Science1678-77652025-02-013310.1590/1678-7757-2024-0392Microbial signatures in head and neck squamous cell carcinoma: an in silico studyLoganathan KAVITHAhttps://orcid.org/0000-0001-7647-0234Manogaran KUZHALMOZHIhttps://orcid.org/0009-0007-4085-6099Jayaseelan VIJAYASHREE PRIYADHARSINIhttps://orcid.org/0000-0001-7884-5466Arunachalam ARUN KUMARKrishna Mohan Rao UMADEVIhttps://orcid.org/0000-0001-9515-5838Kannan RANGANATHANhttps://orcid.org/0000-0003-2149-4145Abstract Objectives The oral cavity harbors a plethora of bacterial species. Dysbiosis of oral and gut microbiota is associated with several oral and systemic pathologies, such as cancer, obesity, diabetes, atherosclerosis and gastrointestinal diseases. Imbalance in the oral-gut microbial axis has been associated with head and neck squamous cell carcinoma (HNSCC). This study aims to analyze the bacterial profile of HNSCC across various taxonomic units, investigate molecular patterns associated with prevalent bacterial phylum in HNSCC, and compare the bacterial profile in HNSCC and gastrointestinal (GI) carcinoma using computational analysis. Methodology The microbe-host transcriptomic, proteomic, and epigenetic analyses of HNSCC and GI carcinomas were performed using The Cancer Microbiome Atlas (TCMA) database. The differential expression of the host’s mRNA transcripts and proteins associated with tumor microbiome were analyzed using The University of Alabama at Birmingham Cancer data analysis (UALCAN) and Clinical Proteomic Tumor Analysis Consortium (CPTAC) websites. Results A decrease in Actinobacteria and an enrichment of Flavobacteria at the class level, Neisseriales, Pasteurellales, and Campylobacterales at the order level, Pasteurellaceae, Flavobacteriaceae, Campylobacteraceae, and Peptoniphilaceae at the family level, and Hemophilus, Porphyromonas, and Leptotrichia at the genus level were observed in HNSCC compared to the normal mucosa. RICTOR protein, mRNA transcripts (HIST1H2BB, SCARNA11, TBC1D21 gene), and hsa-miR-200a-5p miRNA were significantly correlated with prevalent bacterial species in HNSCC. A major increase in Actinobacteria, Fusobacteria, and Spirochaetes was observed in HNSCC compared to GI carcinoma. Conclusion The oral-gut microbial dysbiosis, as reflected by the differential abundance of bacterial species in oral and GI carcinomas, suggests the implication of tumor microbiome and their genomic interactions with the host in carcinogenesis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572025000100405&lng=en&tlng=enMicrobiomeIn silicoOral squamous cell carcinomaHNSCCUALCANTCMA |
| spellingShingle | Loganathan KAVITHA Manogaran KUZHALMOZHI Jayaseelan VIJAYASHREE PRIYADHARSINI Arunachalam ARUN KUMAR Krishna Mohan Rao UMADEVI Kannan RANGANATHAN Microbial signatures in head and neck squamous cell carcinoma: an in silico study Journal of Applied Oral Science Microbiome In silico Oral squamous cell carcinoma HNSCC UALCAN TCMA |
| title | Microbial signatures in head and neck squamous cell carcinoma: an in silico study |
| title_full | Microbial signatures in head and neck squamous cell carcinoma: an in silico study |
| title_fullStr | Microbial signatures in head and neck squamous cell carcinoma: an in silico study |
| title_full_unstemmed | Microbial signatures in head and neck squamous cell carcinoma: an in silico study |
| title_short | Microbial signatures in head and neck squamous cell carcinoma: an in silico study |
| title_sort | microbial signatures in head and neck squamous cell carcinoma an in silico study |
| topic | Microbiome In silico Oral squamous cell carcinoma HNSCC UALCAN TCMA |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572025000100405&lng=en&tlng=en |
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