Liquid chromatography-high resolution mass spectrometry based analysis of persistent mobile organic compounds in aqueous samples: Method development and optimization
Persistent Mobile Organic Compounds (PMOCs) represent a critical threat to water resources due to their high solubility, resistance to degradation, and potential toxicity, posing risks to human health and ecosystems. Their detection and analysis are particularly challenging, necessitating advanced a...
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Elsevier
2025-03-01
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| Series: | Green Analytical Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772577425000114 |
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| author | Kyriaki Anagnostopoulou Vasileios Alampanos Eleni Evgenidou Dimitra A. Lambropoulou |
| author_facet | Kyriaki Anagnostopoulou Vasileios Alampanos Eleni Evgenidou Dimitra A. Lambropoulou |
| author_sort | Kyriaki Anagnostopoulou |
| collection | DOAJ |
| description | Persistent Mobile Organic Compounds (PMOCs) represent a critical threat to water resources due to their high solubility, resistance to degradation, and potential toxicity, posing risks to human health and ecosystems. Their detection and analysis are particularly challenging, necessitating advanced analytical techniques to support regulatory measures and ensure safe drinking water. This study addresses these challenges by developing and optimizing a robust method for PMOC detection, integrating solid-phase extraction with liquid chromatography-high resolution mass spectrometry (SPE-LC-HRMS). By bridging analytical gaps in PMOC identification, the research contributes to water quality protection and aligns with global environmental and public health goals. The method is designed to accommodate the diverse physicochemical properties of PMOCs, enabling the detection of a broad spectrum of compounds. Innovations include the creation of a comprehensive PMOC target list, optimization of sample preparation with specialized SPE cartridges, and the fine-tuning of chromatographic and HRMS parameters. A central composite design and desirability function (DF) approach were used to optimize the HRMS analysis, while the most suitable SPE cartridge, column, and mobile phase composition were carefully selected through experimental trials. The method achieved recoveries within 70–120 % and excellent linearity (r² > 0.997) for all analytes. Validated under European directives (2002/657/EC and SANTE 11,813/2017), the study offers a sensitive, reliable, and regulatory-compliant framework for PMOC monitoring in water systems. By addressing both scientific and regulatory demands, this work advances environmental analytical chemistry and strengthens efforts to protect water resources from emerging contaminants. |
| format | Article |
| id | doaj-art-ca248849bc564b6faf0f0aab9971aa99 |
| institution | Kabale University |
| issn | 2772-5774 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Green Analytical Chemistry |
| spelling | doaj-art-ca248849bc564b6faf0f0aab9971aa992025-02-03T04:17:07ZengElsevierGreen Analytical Chemistry2772-57742025-03-0112100214Liquid chromatography-high resolution mass spectrometry based analysis of persistent mobile organic compounds in aqueous samples: Method development and optimizationKyriaki Anagnostopoulou0Vasileios Alampanos1Eleni Evgenidou2Dimitra A. Lambropoulou3Department of Chemistry, Aristotle University of Thessaloniki, GR 54124, Thessaloniki, Greece; Centre for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center, Thessaloniki, 10th km Thessaloniki-Thermi Rd, GR 57001, GreeceDepartment of Chemistry, Aristotle University of Thessaloniki, GR 54124, Thessaloniki, Greece; Centre for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center, Thessaloniki, 10th km Thessaloniki-Thermi Rd, GR 57001, GreeceDepartment of Chemistry, Aristotle University of Thessaloniki, GR 54124, Thessaloniki, Greece; Centre for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center, Thessaloniki, 10th km Thessaloniki-Thermi Rd, GR 57001, GreeceDepartment of Chemistry, Aristotle University of Thessaloniki, GR 54124, Thessaloniki, Greece; Centre for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center, Thessaloniki, 10th km Thessaloniki-Thermi Rd, GR 57001, Greece; Corresponding author.Persistent Mobile Organic Compounds (PMOCs) represent a critical threat to water resources due to their high solubility, resistance to degradation, and potential toxicity, posing risks to human health and ecosystems. Their detection and analysis are particularly challenging, necessitating advanced analytical techniques to support regulatory measures and ensure safe drinking water. This study addresses these challenges by developing and optimizing a robust method for PMOC detection, integrating solid-phase extraction with liquid chromatography-high resolution mass spectrometry (SPE-LC-HRMS). By bridging analytical gaps in PMOC identification, the research contributes to water quality protection and aligns with global environmental and public health goals. The method is designed to accommodate the diverse physicochemical properties of PMOCs, enabling the detection of a broad spectrum of compounds. Innovations include the creation of a comprehensive PMOC target list, optimization of sample preparation with specialized SPE cartridges, and the fine-tuning of chromatographic and HRMS parameters. A central composite design and desirability function (DF) approach were used to optimize the HRMS analysis, while the most suitable SPE cartridge, column, and mobile phase composition were carefully selected through experimental trials. The method achieved recoveries within 70–120 % and excellent linearity (r² > 0.997) for all analytes. Validated under European directives (2002/657/EC and SANTE 11,813/2017), the study offers a sensitive, reliable, and regulatory-compliant framework for PMOC monitoring in water systems. By addressing both scientific and regulatory demands, this work advances environmental analytical chemistry and strengthens efforts to protect water resources from emerging contaminants.http://www.sciencedirect.com/science/article/pii/S2772577425000114Potentially persistentMobileOrganic compoundsMethod developmentHILICCentral composite design (CCD) |
| spellingShingle | Kyriaki Anagnostopoulou Vasileios Alampanos Eleni Evgenidou Dimitra A. Lambropoulou Liquid chromatography-high resolution mass spectrometry based analysis of persistent mobile organic compounds in aqueous samples: Method development and optimization Green Analytical Chemistry Potentially persistent Mobile Organic compounds Method development HILIC Central composite design (CCD) |
| title | Liquid chromatography-high resolution mass spectrometry based analysis of persistent mobile organic compounds in aqueous samples: Method development and optimization |
| title_full | Liquid chromatography-high resolution mass spectrometry based analysis of persistent mobile organic compounds in aqueous samples: Method development and optimization |
| title_fullStr | Liquid chromatography-high resolution mass spectrometry based analysis of persistent mobile organic compounds in aqueous samples: Method development and optimization |
| title_full_unstemmed | Liquid chromatography-high resolution mass spectrometry based analysis of persistent mobile organic compounds in aqueous samples: Method development and optimization |
| title_short | Liquid chromatography-high resolution mass spectrometry based analysis of persistent mobile organic compounds in aqueous samples: Method development and optimization |
| title_sort | liquid chromatography high resolution mass spectrometry based analysis of persistent mobile organic compounds in aqueous samples method development and optimization |
| topic | Potentially persistent Mobile Organic compounds Method development HILIC Central composite design (CCD) |
| url | http://www.sciencedirect.com/science/article/pii/S2772577425000114 |
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