Menthone lowers H3K27ac levels to inhibit Fusarium proliferatum growth
BackgroundThe antifungal properties of essential oils (EOs) and their active constituents have been well documented. Histone acetylation is pivotal in modulating gene expression and influences biological processes in living organisms.ResultsThis study demonstrated that menthone, the primary componen...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1533918/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832591662306557952 |
|---|---|
| author | Li-Yan Zhang Tian-Tian Li Hong-Xin Liao Jin-Rui Wen Hong-Yan Nie Fu-Rong Xu Xiao-Yun Liu Xian Dong |
| author_facet | Li-Yan Zhang Tian-Tian Li Hong-Xin Liao Jin-Rui Wen Hong-Yan Nie Fu-Rong Xu Xiao-Yun Liu Xian Dong |
| author_sort | Li-Yan Zhang |
| collection | DOAJ |
| description | BackgroundThe antifungal properties of essential oils (EOs) and their active constituents have been well documented. Histone acetylation is pivotal in modulating gene expression and influences biological processes in living organisms.ResultsThis study demonstrated that menthone, the primary component of Mentha haplocalyx EO, exhibited notable antifungal activity against Fusarium proliferatum (EC50 = 6.099 mmol/L). The treatment significantly inhibited hyphal growth, reduced spore germination rates from 31.49 to 4.95%, decreased spore viability from 46.88 to 20.91%, and reduced spore production by a factor of 17.92 compared with the control group while simultaneously enhancing cell membrane permeability. However, the direct relationship between menthone and histone acetylation in inhibiting F. proliferatum remains nebulous. Our RNA sequencing (RNA-seq) analysis identified 7,332 differentially expressed genes (DEGs) between the control and menthone-treated groups, 3,442 upregulated and 3,880 downregulated, primarily enriched in pathways related to ribosome biogenesis and energy metabolism. Chromatin immunoprecipitation sequencing (ChIP-seq) analysis revealed that menthone inhibited the growth of F. proliferatum by decreasing H3K27ac levels and interfering with the transcription of energy metabolism-related genes. By integrating the RNA-seq data with the ChIP-seq results, we identified 110 DEGs associated with reduced H3K27ac modification primarily associated with ribosome biogenesis. Menthone affected the growth of F. proliferatum by reducing the expression of ribosome biogenesis-related genes (FPRO_06392, FPRO_01260, FPRO_10795, and FPRO_01372).ConclusionThis study elucidated the mechanism by which menthone inhibits F. proliferatum growth from a histone acetylation modification perspective, providing insights into its application as an antifungal agent to prevent root rot in Panax notoginseng. |
| format | Article |
| id | doaj-art-ca1e9ed264d6472499b907bbd9946b99 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-ca1e9ed264d6472499b907bbd9946b992025-01-22T07:15:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011610.3389/fmicb.2025.15339181533918Menthone lowers H3K27ac levels to inhibit Fusarium proliferatum growthLi-Yan Zhang0Tian-Tian Li1Hong-Xin Liao2Jin-Rui Wen3Hong-Yan Nie4Fu-Rong Xu5Xiao-Yun Liu6Xian Dong7School of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, ChinaSchool of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, ChinaSchool of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, ChinaSchool of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, ChinaSchool of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, ChinaSchool of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, ChinaHubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, College of Life Sciences, Jianghan University, Wuhan, ChinaSchool of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, ChinaBackgroundThe antifungal properties of essential oils (EOs) and their active constituents have been well documented. Histone acetylation is pivotal in modulating gene expression and influences biological processes in living organisms.ResultsThis study demonstrated that menthone, the primary component of Mentha haplocalyx EO, exhibited notable antifungal activity against Fusarium proliferatum (EC50 = 6.099 mmol/L). The treatment significantly inhibited hyphal growth, reduced spore germination rates from 31.49 to 4.95%, decreased spore viability from 46.88 to 20.91%, and reduced spore production by a factor of 17.92 compared with the control group while simultaneously enhancing cell membrane permeability. However, the direct relationship between menthone and histone acetylation in inhibiting F. proliferatum remains nebulous. Our RNA sequencing (RNA-seq) analysis identified 7,332 differentially expressed genes (DEGs) between the control and menthone-treated groups, 3,442 upregulated and 3,880 downregulated, primarily enriched in pathways related to ribosome biogenesis and energy metabolism. Chromatin immunoprecipitation sequencing (ChIP-seq) analysis revealed that menthone inhibited the growth of F. proliferatum by decreasing H3K27ac levels and interfering with the transcription of energy metabolism-related genes. By integrating the RNA-seq data with the ChIP-seq results, we identified 110 DEGs associated with reduced H3K27ac modification primarily associated with ribosome biogenesis. Menthone affected the growth of F. proliferatum by reducing the expression of ribosome biogenesis-related genes (FPRO_06392, FPRO_01260, FPRO_10795, and FPRO_01372).ConclusionThis study elucidated the mechanism by which menthone inhibits F. proliferatum growth from a histone acetylation modification perspective, providing insights into its application as an antifungal agent to prevent root rot in Panax notoginseng.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1533918/fullantifungal activityribosomeshistone deacetylasesroot rot diseasePanax notoginseng |
| spellingShingle | Li-Yan Zhang Tian-Tian Li Hong-Xin Liao Jin-Rui Wen Hong-Yan Nie Fu-Rong Xu Xiao-Yun Liu Xian Dong Menthone lowers H3K27ac levels to inhibit Fusarium proliferatum growth Frontiers in Microbiology antifungal activity ribosomes histone deacetylases root rot disease Panax notoginseng |
| title | Menthone lowers H3K27ac levels to inhibit Fusarium proliferatum growth |
| title_full | Menthone lowers H3K27ac levels to inhibit Fusarium proliferatum growth |
| title_fullStr | Menthone lowers H3K27ac levels to inhibit Fusarium proliferatum growth |
| title_full_unstemmed | Menthone lowers H3K27ac levels to inhibit Fusarium proliferatum growth |
| title_short | Menthone lowers H3K27ac levels to inhibit Fusarium proliferatum growth |
| title_sort | menthone lowers h3k27ac levels to inhibit fusarium proliferatum growth |
| topic | antifungal activity ribosomes histone deacetylases root rot disease Panax notoginseng |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1533918/full |
| work_keys_str_mv | AT liyanzhang menthonelowersh3k27aclevelstoinhibitfusariumproliferatumgrowth AT tiantianli menthonelowersh3k27aclevelstoinhibitfusariumproliferatumgrowth AT hongxinliao menthonelowersh3k27aclevelstoinhibitfusariumproliferatumgrowth AT jinruiwen menthonelowersh3k27aclevelstoinhibitfusariumproliferatumgrowth AT hongyannie menthonelowersh3k27aclevelstoinhibitfusariumproliferatumgrowth AT furongxu menthonelowersh3k27aclevelstoinhibitfusariumproliferatumgrowth AT xiaoyunliu menthonelowersh3k27aclevelstoinhibitfusariumproliferatumgrowth AT xiandong menthonelowersh3k27aclevelstoinhibitfusariumproliferatumgrowth |