Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study
Olive leaf extract is characterized by a high content of polyphenols (oleuropein, hydroxytyrosol and their derivatives), which is associated with its therapeutic properties. The objective of the present research was to evaluate the antifungal activity of olive leaf extract against Candida albicans A...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2016-09-01
|
| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/acph-2016-0033 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832571955749847040 |
|---|---|
| author | Zorić Nataša Kopjar Nevenka Kraljić Klara Oršolić Nada Tomić Siniša Kosalec Ivan |
| author_facet | Zorić Nataša Kopjar Nevenka Kraljić Klara Oršolić Nada Tomić Siniša Kosalec Ivan |
| author_sort | Zorić Nataša |
| collection | DOAJ |
| description | Olive leaf extract is characterized by a high content of polyphenols (oleuropein, hydroxytyrosol and their derivatives), which is associated with its therapeutic properties. The objective of the present research was to evaluate the antifungal activity of olive leaf extract against Candida albicans ATCC 10231 and C. dubliniensis CBS 7987 strains. Minimum inhibitory concentrations (MIC) of the extract were determined by several in vitro assays. The extract showed a concentration depended effect on the viability of C. albicans with MIC value of 46.875 mg mL-1 and C. dubliniensis with MIC value 62.5 mg mL-1. Most sensitive methods for testing the antifungal effect of the extracts were the trypan blue exclusion method and fluorescent dye exclusion method while MIC could not be determined by the method according to the EUCAST recommendation suggesting that herbal preparations contain compounds that may interfere with this susceptibility testing. The fluorescent dye exclusion method was also used for the assessment of morphological changes in the nuclei of treated cells. According to the obtained results, olive leaf extract is less effective against the tested strains than hydroxytyrosol, an olive plant constituent tested in our previous study. |
| format | Article |
| id | doaj-art-ca17aeb918484afe9cbcef87fe2f8a41 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2016-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-ca17aeb918484afe9cbcef87fe2f8a412025-02-02T12:11:59ZengSciendoActa Pharmaceutica1846-95582016-09-0166341142110.1515/acph-2016-0033acph-2016-0033Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability studyZorić Nataša0Kopjar Nevenka1Kraljić Klara2Oršolić Nada3Tomić Siniša4Kosalec Ivan5Agency for Medicinal Products and Medical Devices of Croatia (HALMED) 10 000 Zagreb, CroatiaInstitute for Medical Research and Occupational Health 10 000 Zagreb, CroatiaUniversity of Zagreb Faculty of Food Technology and Biotechnology 10 000 Zagreb, CroatiaUniversity of Zagreb Faculty of Science Division of Biology 10 000 Zagreb, CroatiaAgency for Medicinal Products and Medical Devices of Croatia (HALMED) 10 000 Zagreb, CroatiaAgency for Medicinal Products and Medical Devices of Croatia (HALMED) 10 000 Zagreb, CroatiaOlive leaf extract is characterized by a high content of polyphenols (oleuropein, hydroxytyrosol and their derivatives), which is associated with its therapeutic properties. The objective of the present research was to evaluate the antifungal activity of olive leaf extract against Candida albicans ATCC 10231 and C. dubliniensis CBS 7987 strains. Minimum inhibitory concentrations (MIC) of the extract were determined by several in vitro assays. The extract showed a concentration depended effect on the viability of C. albicans with MIC value of 46.875 mg mL-1 and C. dubliniensis with MIC value 62.5 mg mL-1. Most sensitive methods for testing the antifungal effect of the extracts were the trypan blue exclusion method and fluorescent dye exclusion method while MIC could not be determined by the method according to the EUCAST recommendation suggesting that herbal preparations contain compounds that may interfere with this susceptibility testing. The fluorescent dye exclusion method was also used for the assessment of morphological changes in the nuclei of treated cells. According to the obtained results, olive leaf extract is less effective against the tested strains than hydroxytyrosol, an olive plant constituent tested in our previous study.https://doi.org/10.1515/acph-2016-0033antifungal activitycandida albicanscandida dubliniensisolive leaf extract |
| spellingShingle | Zorić Nataša Kopjar Nevenka Kraljić Klara Oršolić Nada Tomić Siniša Kosalec Ivan Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study Acta Pharmaceutica antifungal activity candida albicans candida dubliniensis olive leaf extract |
| title | Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study |
| title_full | Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study |
| title_fullStr | Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study |
| title_full_unstemmed | Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study |
| title_short | Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study |
| title_sort | olive leaf extract activity against candida albicans and c dubliniensis the in vitro viability study |
| topic | antifungal activity candida albicans candida dubliniensis olive leaf extract |
| url | https://doi.org/10.1515/acph-2016-0033 |
| work_keys_str_mv | AT zoricnatasa oliveleafextractactivityagainstcandidaalbicansandcdubliniensistheinvitroviabilitystudy AT kopjarnevenka oliveleafextractactivityagainstcandidaalbicansandcdubliniensistheinvitroviabilitystudy AT kraljicklara oliveleafextractactivityagainstcandidaalbicansandcdubliniensistheinvitroviabilitystudy AT orsolicnada oliveleafextractactivityagainstcandidaalbicansandcdubliniensistheinvitroviabilitystudy AT tomicsinisa oliveleafextractactivityagainstcandidaalbicansandcdubliniensistheinvitroviabilitystudy AT kosalecivan oliveleafextractactivityagainstcandidaalbicansandcdubliniensistheinvitroviabilitystudy |