Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer Cells
Background/Aim. Grammatophyllum speciosum Blume exhibits various promising pharmacological activities. However, its effect on breast cancer has not been determined. Materials and Methods. The antiproliferation effects of the G. speciosum pseudobulb ethanolic extract (GSE) and isovitexin (bioactive c...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2021/3752169 |
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| author | Verisa Chowjarean Kamala Sadabpod |
| author_facet | Verisa Chowjarean Kamala Sadabpod |
| author_sort | Verisa Chowjarean |
| collection | DOAJ |
| description | Background/Aim. Grammatophyllum speciosum Blume exhibits various promising pharmacological activities. However, its effect on breast cancer has not been determined. Materials and Methods. The antiproliferation effects of the G. speciosum pseudobulb ethanolic extract (GSE) and isovitexin (bioactive constituent) were investigated on the MCF-7 human breast cancer cell line using MTT and colony formation assay. The expression levels of proliferation-regulatory proteins were determined by western blotting. Results. Noncytotoxic concentrations of GSE significantly suppressed the proliferation of MCF-7 cells. Tumor colony formation decreased in both number and size. The level of phosphorylated AKT and β-catenin was suppressed by GSE treatment. Antiproliferation was observed in isovitexin-treated MCF-7 cells in the form of inhibited colony formation and reduced expression of phosphorylated AKT and β-catenin protein. Conclusions. This study demonstrates the novel effect of G. speciosum as an antiproliferative via suppression of the AKT/β-catenin-dependent pathway. This may prompt further investigation of this plant in breast cancer therapy. |
| format | Article |
| id | doaj-art-c9f7842b26fa4fc4a316bf950b50ceac |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-c9f7842b26fa4fc4a316bf950b50ceac2025-02-03T07:23:31ZengWileyThe Scientific World Journal2356-61401537-744X2021-01-01202110.1155/2021/37521693752169Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer CellsVerisa Chowjarean0Kamala Sadabpod1Cosmeceutical Research, Development and Testing Center, College of Pharmacy, Rangsit University, Lak Hok, Pathum Thani 12000, ThailandDepartment of Pharmacy Practice, College of Pharmacy, Rangsit University, Lak Hok, Pathum Thani 12000, ThailandBackground/Aim. Grammatophyllum speciosum Blume exhibits various promising pharmacological activities. However, its effect on breast cancer has not been determined. Materials and Methods. The antiproliferation effects of the G. speciosum pseudobulb ethanolic extract (GSE) and isovitexin (bioactive constituent) were investigated on the MCF-7 human breast cancer cell line using MTT and colony formation assay. The expression levels of proliferation-regulatory proteins were determined by western blotting. Results. Noncytotoxic concentrations of GSE significantly suppressed the proliferation of MCF-7 cells. Tumor colony formation decreased in both number and size. The level of phosphorylated AKT and β-catenin was suppressed by GSE treatment. Antiproliferation was observed in isovitexin-treated MCF-7 cells in the form of inhibited colony formation and reduced expression of phosphorylated AKT and β-catenin protein. Conclusions. This study demonstrates the novel effect of G. speciosum as an antiproliferative via suppression of the AKT/β-catenin-dependent pathway. This may prompt further investigation of this plant in breast cancer therapy.http://dx.doi.org/10.1155/2021/3752169 |
| spellingShingle | Verisa Chowjarean Kamala Sadabpod Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer Cells The Scientific World Journal |
| title | Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer Cells |
| title_full | Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer Cells |
| title_fullStr | Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer Cells |
| title_full_unstemmed | Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer Cells |
| title_short | Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer Cells |
| title_sort | antiproliferative effect of grammatophyllum speciosum ethanolic extract and its bioactive compound on human breast cancer cells |
| url | http://dx.doi.org/10.1155/2021/3752169 |
| work_keys_str_mv | AT verisachowjarean antiproliferativeeffectofgrammatophyllumspeciosumethanolicextractanditsbioactivecompoundonhumanbreastcancercells AT kamalasadabpod antiproliferativeeffectofgrammatophyllumspeciosumethanolicextractanditsbioactivecompoundonhumanbreastcancercells |