Molecular characterization and lineage analysis of canine astrovirus strains from dogs with gastrointestinal disease in Ecuador based on ORF-2 gene analysis
Canine Astrovirus (CaAstV) part of the Astroviridae family and genus Mamastrovirus, is a linear RNA virus with a genome of approximately 6.6 kb with three open reading frames (ORF): ORF1a and ORF1b, which code for the most conserved non-structural proteins, and ORF2, which code for the capsid protei...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1505903/full |
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| author | Anthony Loor-Giler Anthony Loor-Giler Silvana Santander-Parra Sara Castillo-Reyes Martín Campos Martín Campos Renan Mena-Perez Renan Mena-Perez Santiago Prado-Chiriboga Santiago Prado-Chiriboga Luis Nunez Luis Nunez |
| author_facet | Anthony Loor-Giler Anthony Loor-Giler Silvana Santander-Parra Sara Castillo-Reyes Martín Campos Martín Campos Renan Mena-Perez Renan Mena-Perez Santiago Prado-Chiriboga Santiago Prado-Chiriboga Luis Nunez Luis Nunez |
| author_sort | Anthony Loor-Giler |
| collection | DOAJ |
| description | Canine Astrovirus (CaAstV) part of the Astroviridae family and genus Mamastrovirus, is a linear RNA virus with a genome of approximately 6.6 kb with three open reading frames (ORF): ORF1a and ORF1b, which code for the most conserved non-structural proteins, and ORF2, which code for the capsid protein, the most variable region of the genome. This pathogen has been linked to gastrointestinal infections, primarily causing symptoms such as vomiting, diarrhea, lethargy and severe dehydration, mainly in co-infection with other enteric viruses. In the present study, the presence of CaAstV was identified in dogs with gastrointestinal disease in Ecuador using RT-qPCR with hydrolysis probes, these samples have also tested positive for canine parvovirus type 2 (CPV-2) and canine coronavirus (CCoV). Positive samples were used for end-point RT-PCR amplification and sequencing of ORF-2 using Sanger technology. The sequences were subjected to phylogenetic analysis to determine lineages and possible recombination events. Of the 502 samples tested, 336 were found to be positive for CaAstV, 49.4% in co-infection with CPV-2, 1% in co-infection with CCoV, and 4% in simultaneous infection with all three viruses. The presence of 4 of the 5 previously reported CaAstV lineages were identified, and three possible recombinant strains were identified. Given the high frequency of CaAstV infections in dogs with gastroenteritis and its high genetic variability, it emphasizes the need to implement routine diagnostic measures that include this pathogen as one of the main causes of the disease and a risk agent in case of multiple infections. |
| format | Article |
| id | doaj-art-c9f0334f89684899bcf25d061b460cad |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-c9f0334f89684899bcf25d061b460cad2025-02-03T15:07:40ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-02-011110.3389/fvets.2024.15059031505903Molecular characterization and lineage analysis of canine astrovirus strains from dogs with gastrointestinal disease in Ecuador based on ORF-2 gene analysisAnthony Loor-Giler0Anthony Loor-Giler1Silvana Santander-Parra2Sara Castillo-Reyes3Martín Campos4Martín Campos5Renan Mena-Perez6Renan Mena-Perez7Santiago Prado-Chiriboga8Santiago Prado-Chiriboga9Luis Nunez10Luis Nunez11Laboratorios de Investigación, Dirección General de Investigación, Universidad de las Américas (UDLA), Quito, EcuadorFacultad de Ingeniería y Ciencias Aplicadas, Carrera de Ingeniería en Biotecnología, Universidad de Las Américas (UDLA), Quito, EcuadorFacultad de Ciencias de la Salud, Carrera de Medicina Veterinaria, Universidad de Las Americas (UDLA), Quito, EcuadorFacultad de Ciencias de la Salud, Carrera de Medicina Veterinaria, Universidad de Las Americas (UDLA), Quito, EcuadorFacultad de Industrias Agropecuarias y Ciencias Ambientales, Carrera Agropecuaria, Universidad Politécnica Estatal del Carchi (UPEC), Tulcán, EcuadorFacultad de Ciencias Veterinarias, Universidad Nacional de Rosario (UNR), Santa Fe, ArgentinaFacultad de Ciencias de la Salud, Carrera de Medicina Veterinaria, Universidad de Las Americas (UDLA), Quito, EcuadorFacultad de Medicina Veterinaria y Zootecnia, Universidad Central Del Ecuador, Quito, EcuadorFacultad de Ciencias de la Salud, Carrera de Medicina Veterinaria, Universidad de Las Americas (UDLA), Quito, EcuadorClínica Veterinaria Docente, Universidad de Las Américas (UDLA), Quito, EcuadorFacultad de Ciencias de la Salud, Carrera de Medicina Veterinaria, Universidad de Las Americas (UDLA), Quito, EcuadorOne Health Research Group, Facultad de Ciencias de la Salud, Universidad de Las Américas (UDLA), Quito, EcuadorCanine Astrovirus (CaAstV) part of the Astroviridae family and genus Mamastrovirus, is a linear RNA virus with a genome of approximately 6.6 kb with three open reading frames (ORF): ORF1a and ORF1b, which code for the most conserved non-structural proteins, and ORF2, which code for the capsid protein, the most variable region of the genome. This pathogen has been linked to gastrointestinal infections, primarily causing symptoms such as vomiting, diarrhea, lethargy and severe dehydration, mainly in co-infection with other enteric viruses. In the present study, the presence of CaAstV was identified in dogs with gastrointestinal disease in Ecuador using RT-qPCR with hydrolysis probes, these samples have also tested positive for canine parvovirus type 2 (CPV-2) and canine coronavirus (CCoV). Positive samples were used for end-point RT-PCR amplification and sequencing of ORF-2 using Sanger technology. The sequences were subjected to phylogenetic analysis to determine lineages and possible recombination events. Of the 502 samples tested, 336 were found to be positive for CaAstV, 49.4% in co-infection with CPV-2, 1% in co-infection with CCoV, and 4% in simultaneous infection with all three viruses. The presence of 4 of the 5 previously reported CaAstV lineages were identified, and three possible recombinant strains were identified. Given the high frequency of CaAstV infections in dogs with gastroenteritis and its high genetic variability, it emphasizes the need to implement routine diagnostic measures that include this pathogen as one of the main causes of the disease and a risk agent in case of multiple infections.https://www.frontiersin.org/articles/10.3389/fvets.2024.1505903/fullCaAstVgastroenteric diseaseRT-qPCRlinagesprotein structure |
| spellingShingle | Anthony Loor-Giler Anthony Loor-Giler Silvana Santander-Parra Sara Castillo-Reyes Martín Campos Martín Campos Renan Mena-Perez Renan Mena-Perez Santiago Prado-Chiriboga Santiago Prado-Chiriboga Luis Nunez Luis Nunez Molecular characterization and lineage analysis of canine astrovirus strains from dogs with gastrointestinal disease in Ecuador based on ORF-2 gene analysis Frontiers in Veterinary Science CaAstV gastroenteric disease RT-qPCR linages protein structure |
| title | Molecular characterization and lineage analysis of canine astrovirus strains from dogs with gastrointestinal disease in Ecuador based on ORF-2 gene analysis |
| title_full | Molecular characterization and lineage analysis of canine astrovirus strains from dogs with gastrointestinal disease in Ecuador based on ORF-2 gene analysis |
| title_fullStr | Molecular characterization and lineage analysis of canine astrovirus strains from dogs with gastrointestinal disease in Ecuador based on ORF-2 gene analysis |
| title_full_unstemmed | Molecular characterization and lineage analysis of canine astrovirus strains from dogs with gastrointestinal disease in Ecuador based on ORF-2 gene analysis |
| title_short | Molecular characterization and lineage analysis of canine astrovirus strains from dogs with gastrointestinal disease in Ecuador based on ORF-2 gene analysis |
| title_sort | molecular characterization and lineage analysis of canine astrovirus strains from dogs with gastrointestinal disease in ecuador based on orf 2 gene analysis |
| topic | CaAstV gastroenteric disease RT-qPCR linages protein structure |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1505903/full |
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