<i>CaHY5</i> Mediates UV-B Induced Anthocyanin Biosynthesis in Purple Pepper
Anthocyanins are important flavonoid compounds in plants that are associated with the color formation and antioxidant activity of flowers, fruits, and other organs. Ultraviolet B radiation (UV-B) is one of the key environmental factors that influence anthocyanin accumulation in plants and <i>H...
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2024-12-01
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| author | Xiang Zhang Yunrong Mo Huidan Zhou Mengjuan Li Hong Cheng Pingping Li Ruihao Zhang Yaoyao Huang Yanyan Wang Junqiang Xu Jingjing Liao Qiaoli Xie Kai Zhao Minghua Deng Junheng Lv |
| author_facet | Xiang Zhang Yunrong Mo Huidan Zhou Mengjuan Li Hong Cheng Pingping Li Ruihao Zhang Yaoyao Huang Yanyan Wang Junqiang Xu Jingjing Liao Qiaoli Xie Kai Zhao Minghua Deng Junheng Lv |
| author_sort | Xiang Zhang |
| collection | DOAJ |
| description | Anthocyanins are important flavonoid compounds in plants that are associated with the color formation and antioxidant activity of flowers, fruits, and other organs. Ultraviolet B radiation (UV-B) is one of the key environmental factors that influence anthocyanin accumulation in plants and <i>HY5</i> is involved in plant photomorphogenesis. However, the molecular mechanism of the signal network of UV-B regulating anthocyanin biosynthesis in capsicum via <i>HY5</i> remains unclear. In this study, we identified the transcription factor <i>CaHY5</i>, which mediates UV-B signaling, and demonstrated its regulatory role in anthocyanin biosynthesis in purple pepper (<i>Capsicum annuum</i> L.). The results showed that there were photoresponsive and hormone-responsive elements on the <i>CaHY5</i> promoter that responded to UV-B, indoleacetic acid, salicylic acid, 6-benzyladenine, abscisic acid, and melatonin treatments. UV-B treatment induced the expression of <i>CaHY5</i> and anthocyanin structural genes. <i>CaHY5</i> gene-silenced positive plants showed different degrees of the yellowing phenomenon, which affected the expression of the anthocyanin biosynthesis structural gene. The expression levels of anthocyanin biosynthesis-related genes in <i>CaHY5</i>-silenced positive plants increased considerably. This study provides insights into the role of <i>CaHY5</i> in UV-B-induced anthocyanin biosynthesis in purple pepper. |
| format | Article |
| id | doaj-art-c9d0f9d63c274eaea6e8e55c0456f788 |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-c9d0f9d63c274eaea6e8e55c0456f7882025-01-24T13:16:24ZengMDPI AGAgronomy2073-43952024-12-011512810.3390/agronomy15010028<i>CaHY5</i> Mediates UV-B Induced Anthocyanin Biosynthesis in Purple PepperXiang Zhang0Yunrong Mo1Huidan Zhou2Mengjuan Li3Hong Cheng4Pingping Li5Ruihao Zhang6Yaoyao Huang7Yanyan Wang8Junqiang Xu9Jingjing Liao10Qiaoli Xie11Kai Zhao12Minghua Deng13Junheng Lv14Key Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Bioengineering, Chongqing University, Chongqing 400044, ChinaKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaAnthocyanins are important flavonoid compounds in plants that are associated with the color formation and antioxidant activity of flowers, fruits, and other organs. Ultraviolet B radiation (UV-B) is one of the key environmental factors that influence anthocyanin accumulation in plants and <i>HY5</i> is involved in plant photomorphogenesis. However, the molecular mechanism of the signal network of UV-B regulating anthocyanin biosynthesis in capsicum via <i>HY5</i> remains unclear. In this study, we identified the transcription factor <i>CaHY5</i>, which mediates UV-B signaling, and demonstrated its regulatory role in anthocyanin biosynthesis in purple pepper (<i>Capsicum annuum</i> L.). The results showed that there were photoresponsive and hormone-responsive elements on the <i>CaHY5</i> promoter that responded to UV-B, indoleacetic acid, salicylic acid, 6-benzyladenine, abscisic acid, and melatonin treatments. UV-B treatment induced the expression of <i>CaHY5</i> and anthocyanin structural genes. <i>CaHY5</i> gene-silenced positive plants showed different degrees of the yellowing phenomenon, which affected the expression of the anthocyanin biosynthesis structural gene. The expression levels of anthocyanin biosynthesis-related genes in <i>CaHY5</i>-silenced positive plants increased considerably. This study provides insights into the role of <i>CaHY5</i> in UV-B-induced anthocyanin biosynthesis in purple pepper.https://www.mdpi.com/2073-4395/15/1/28<i>Capsicum annuum</i> L.<i>CaHY5</i> geneabiotic stressanthocyanin biosynthesis |
| spellingShingle | Xiang Zhang Yunrong Mo Huidan Zhou Mengjuan Li Hong Cheng Pingping Li Ruihao Zhang Yaoyao Huang Yanyan Wang Junqiang Xu Jingjing Liao Qiaoli Xie Kai Zhao Minghua Deng Junheng Lv <i>CaHY5</i> Mediates UV-B Induced Anthocyanin Biosynthesis in Purple Pepper Agronomy <i>Capsicum annuum</i> L. <i>CaHY5</i> gene abiotic stress anthocyanin biosynthesis |
| title | <i>CaHY5</i> Mediates UV-B Induced Anthocyanin Biosynthesis in Purple Pepper |
| title_full | <i>CaHY5</i> Mediates UV-B Induced Anthocyanin Biosynthesis in Purple Pepper |
| title_fullStr | <i>CaHY5</i> Mediates UV-B Induced Anthocyanin Biosynthesis in Purple Pepper |
| title_full_unstemmed | <i>CaHY5</i> Mediates UV-B Induced Anthocyanin Biosynthesis in Purple Pepper |
| title_short | <i>CaHY5</i> Mediates UV-B Induced Anthocyanin Biosynthesis in Purple Pepper |
| title_sort | i cahy5 i mediates uv b induced anthocyanin biosynthesis in purple pepper |
| topic | <i>Capsicum annuum</i> L. <i>CaHY5</i> gene abiotic stress anthocyanin biosynthesis |
| url | https://www.mdpi.com/2073-4395/15/1/28 |
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