Non-viral systems for intracellular delivery of genome editing tools
A hallmark of the last decades is an extensive development of genome editing systems and technologies propelling genetic engineering to the next level. Specific and efficient delivery of genome editing tools to target cells is one of the key elements of such technologies. Conventional vectors are no...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2024-04-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/4094 |
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| author | I. H. Shaikhutdinov P. V. Ilyasov O. V. Gribkova L. V. Limareva |
| author_facet | I. H. Shaikhutdinov P. V. Ilyasov O. V. Gribkova L. V. Limareva |
| author_sort | I. H. Shaikhutdinov |
| collection | DOAJ |
| description | A hallmark of the last decades is an extensive development of genome editing systems and technologies propelling genetic engineering to the next level. Specific and efficient delivery of genome editing tools to target cells is one of the key elements of such technologies. Conventional vectors are not always suitable for this purpose due to a limited cargo volume, risks related to cancer and immune reactions, toxicity, a need for high-purity viral material and quality control, as well as a possibility of integration of the virus into the host genome leading to overexpression of the vector components and safety problems. Therefore, the search for novel approaches to delivering proteins and nucleic acids into cells is a relevant priority. This work reviews abiotic vectors and systems for delivering genome editing tools into target cells, including liposomes and solid lipid particles, other membrane-based vesicles, cell-penetrating peptides, micelles, dendrimers, carbon nanotubes, inorganic, polymer, metal and other nanoparticles. It considers advantages, drawbacks and preferred applications of such systems as well as suitability thereof for the delivery of genome editing systems. A particular emphasis is placed on metal-organic frameworks (MOFs) and their potential in the targeted intracellular delivery of proteins and polynucleotides. It has been concluded that further development of MOF-based vectors and technologies, as well as combining MOFs with other carriers can result in safe and efficient delivery systems, which would be able to circulate in the body for a long time while recognizing target cells and ensuring cell-specific delivery and release of intact cargoes and, thereby, improving the genome editing outcome. |
| format | Article |
| id | doaj-art-c9ccc75028a24fdfb002c0c5de33cfb3 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-c9ccc75028a24fdfb002c0c5de33cfb32025-02-01T09:58:13ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592024-04-0128223924810.18699/vjgb-24-281459Non-viral systems for intracellular delivery of genome editing toolsI. H. Shaikhutdinov0P. V. Ilyasov1O. V. Gribkova2L. V. Limareva3Samara State Medical University of the Ministry of Healthcare of the Russian FederationSamara State Medical University of the Ministry of Healthcare of the Russian FederationSamara State Medical University of the Ministry of Healthcare of the Russian FederationSamara State Medical University of the Ministry of Healthcare of the Russian FederationA hallmark of the last decades is an extensive development of genome editing systems and technologies propelling genetic engineering to the next level. Specific and efficient delivery of genome editing tools to target cells is one of the key elements of such technologies. Conventional vectors are not always suitable for this purpose due to a limited cargo volume, risks related to cancer and immune reactions, toxicity, a need for high-purity viral material and quality control, as well as a possibility of integration of the virus into the host genome leading to overexpression of the vector components and safety problems. Therefore, the search for novel approaches to delivering proteins and nucleic acids into cells is a relevant priority. This work reviews abiotic vectors and systems for delivering genome editing tools into target cells, including liposomes and solid lipid particles, other membrane-based vesicles, cell-penetrating peptides, micelles, dendrimers, carbon nanotubes, inorganic, polymer, metal and other nanoparticles. It considers advantages, drawbacks and preferred applications of such systems as well as suitability thereof for the delivery of genome editing systems. A particular emphasis is placed on metal-organic frameworks (MOFs) and their potential in the targeted intracellular delivery of proteins and polynucleotides. It has been concluded that further development of MOF-based vectors and technologies, as well as combining MOFs with other carriers can result in safe and efficient delivery systems, which would be able to circulate in the body for a long time while recognizing target cells and ensuring cell-specific delivery and release of intact cargoes and, thereby, improving the genome editing outcome.https://vavilov.elpub.ru/jour/article/view/4094metal-organic frameworksvesiclesnanoparticlesviral vectorsgene editing |
| spellingShingle | I. H. Shaikhutdinov P. V. Ilyasov O. V. Gribkova L. V. Limareva Non-viral systems for intracellular delivery of genome editing tools Вавиловский журнал генетики и селекции metal-organic frameworks vesicles nanoparticles viral vectors gene editing |
| title | Non-viral systems for intracellular delivery of genome editing tools |
| title_full | Non-viral systems for intracellular delivery of genome editing tools |
| title_fullStr | Non-viral systems for intracellular delivery of genome editing tools |
| title_full_unstemmed | Non-viral systems for intracellular delivery of genome editing tools |
| title_short | Non-viral systems for intracellular delivery of genome editing tools |
| title_sort | non viral systems for intracellular delivery of genome editing tools |
| topic | metal-organic frameworks vesicles nanoparticles viral vectors gene editing |
| url | https://vavilov.elpub.ru/jour/article/view/4094 |
| work_keys_str_mv | AT ihshaikhutdinov nonviralsystemsforintracellulardeliveryofgenomeeditingtools AT pvilyasov nonviralsystemsforintracellulardeliveryofgenomeeditingtools AT ovgribkova nonviralsystemsforintracellulardeliveryofgenomeeditingtools AT lvlimareva nonviralsystemsforintracellulardeliveryofgenomeeditingtools |