Mechanisms of Cytotoxicity of Chemical Agents to Giant Cell Tumors: An In Vitro Study
Background. Various chemical agents have been used as an adjuvant treatment for giant cell tumor (GCT). However, the comparative effect of these chemicals remains unclear. Methods. Multinucleated and spindle cells from cultured GCT patients, characterized by Nanog and Oct4 expression with RT-PCR, we...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2020/8827192 |
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| author | Achmad Fauzi Kamal Akbar Rizki Beni Asdi Ahmad Jabir Rahyussalim Rio Wikanjaya Resda Akhra Syahrani Tri Kurniawati Septelia Inawati Wanandi |
| author_facet | Achmad Fauzi Kamal Akbar Rizki Beni Asdi Ahmad Jabir Rahyussalim Rio Wikanjaya Resda Akhra Syahrani Tri Kurniawati Septelia Inawati Wanandi |
| author_sort | Achmad Fauzi Kamal |
| collection | DOAJ |
| description | Background. Various chemical agents have been used as an adjuvant treatment for giant cell tumor (GCT). However, the comparative effect of these chemicals remains unclear. Methods. Multinucleated and spindle cells from cultured GCT patients, characterized by Nanog and Oct4 expression with RT-PCR, were directly administered, in vitro, with concentrations of 1%, 3%, and 5% of H2O2 and 75%, 85%, and 95% of ethanol for 10 minutes and concentrations of 0.003%, 0.005%, 0.01%, 0.03%, 0.1%, and 0.3% of H2O2 for 5 minutes and were incubated for 24 hours. Cell morphology, cell viability, and flow cytometry after various concentrations of H2O2 and ethanol exposure were assessed. Results. H2O2 in all concentrations caused loss of cell viability. The number of viable cells after H2O2 exposure was related to the concentration-dependent effect. The initial viable spindle-shaped cell, multinucleated giant cell, and round-epithelioid cell had morphological changes into fragmented nonviable cells after exposure to H2O2. Flow cytometry using Annexin V showed cell death due to necrosis, with the highest concentration amounting to 0.3%. Conclusion. Administering local chemical adjuvants of H2O2 in vitro caused loss of viable GCT cells. The number of viable cells after H2O2 exposure was related to the concentration-dependent effect, whereas reducing concentration of H2O2 may cause loss of viability and morphology of cultured GCT cells with the apoptotic mechanism. |
| format | Article |
| id | doaj-art-c9c34df726ad466bb298705c40192978 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-c9c34df726ad466bb298705c401929782025-02-03T01:04:23ZengWileyStem Cells International1687-966X1687-96782020-01-01202010.1155/2020/88271928827192Mechanisms of Cytotoxicity of Chemical Agents to Giant Cell Tumors: An In Vitro StudyAchmad Fauzi Kamal0Akbar Rizki Beni Asdi1Ahmad Jabir Rahyussalim2Rio Wikanjaya3Resda Akhra Syahrani4Tri Kurniawati5Septelia Inawati Wanandi6Department of Orthopaedic and Traumatology, Faculty of Medicine Universitas Indonesia/Cipto Mangunkusumo General Hospital, Jakarta, IndonesiaDepartment of Orthopaedic and Traumatology, Faculty of Medicine Universitas Indonesia/Cipto Mangunkusumo General Hospital, Jakarta, IndonesiaDepartment of Orthopaedic and Traumatology, Faculty of Medicine Universitas Indonesia/Cipto Mangunkusumo General Hospital, Jakarta, IndonesiaDepartment of Orthopaedic and Traumatology, Faculty of Medicine Universitas Indonesia/Cipto Mangunkusumo General Hospital, Jakarta, IndonesiaLaboratorium of Stem Cell Research, Faculty of Medicine Universitas Indonesia, Jakarta, IndonesiaStem Cell Integrated Medical Technology Unit, Cipto Mangunkusumo General Hospital, Jakarta, IndonesiaLaboratorium of Stem Cell Research, Faculty of Medicine Universitas Indonesia, Jakarta, IndonesiaBackground. Various chemical agents have been used as an adjuvant treatment for giant cell tumor (GCT). However, the comparative effect of these chemicals remains unclear. Methods. Multinucleated and spindle cells from cultured GCT patients, characterized by Nanog and Oct4 expression with RT-PCR, were directly administered, in vitro, with concentrations of 1%, 3%, and 5% of H2O2 and 75%, 85%, and 95% of ethanol for 10 minutes and concentrations of 0.003%, 0.005%, 0.01%, 0.03%, 0.1%, and 0.3% of H2O2 for 5 minutes and were incubated for 24 hours. Cell morphology, cell viability, and flow cytometry after various concentrations of H2O2 and ethanol exposure were assessed. Results. H2O2 in all concentrations caused loss of cell viability. The number of viable cells after H2O2 exposure was related to the concentration-dependent effect. The initial viable spindle-shaped cell, multinucleated giant cell, and round-epithelioid cell had morphological changes into fragmented nonviable cells after exposure to H2O2. Flow cytometry using Annexin V showed cell death due to necrosis, with the highest concentration amounting to 0.3%. Conclusion. Administering local chemical adjuvants of H2O2 in vitro caused loss of viable GCT cells. The number of viable cells after H2O2 exposure was related to the concentration-dependent effect, whereas reducing concentration of H2O2 may cause loss of viability and morphology of cultured GCT cells with the apoptotic mechanism.http://dx.doi.org/10.1155/2020/8827192 |
| spellingShingle | Achmad Fauzi Kamal Akbar Rizki Beni Asdi Ahmad Jabir Rahyussalim Rio Wikanjaya Resda Akhra Syahrani Tri Kurniawati Septelia Inawati Wanandi Mechanisms of Cytotoxicity of Chemical Agents to Giant Cell Tumors: An In Vitro Study Stem Cells International |
| title | Mechanisms of Cytotoxicity of Chemical Agents to Giant Cell Tumors: An In Vitro Study |
| title_full | Mechanisms of Cytotoxicity of Chemical Agents to Giant Cell Tumors: An In Vitro Study |
| title_fullStr | Mechanisms of Cytotoxicity of Chemical Agents to Giant Cell Tumors: An In Vitro Study |
| title_full_unstemmed | Mechanisms of Cytotoxicity of Chemical Agents to Giant Cell Tumors: An In Vitro Study |
| title_short | Mechanisms of Cytotoxicity of Chemical Agents to Giant Cell Tumors: An In Vitro Study |
| title_sort | mechanisms of cytotoxicity of chemical agents to giant cell tumors an in vitro study |
| url | http://dx.doi.org/10.1155/2020/8827192 |
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