Generation of Inheritable A-to-G Transitions Using Adenine Base Editing and NG-PAM Cas9 in Arabidopsis thaliana

Generation of Inheritable A-to-G Transitions Using Adenine Base Editing and NG-PAM Cas9 in Arabidopsis thaliana

Towards precise genome editing, base editors have been developed by fusing catalytically compromised Cas9 with deaminase components, mediating C-to-T (cytosine base editors) or A-to-G (adenine base editors) transition. We developed a set of vectors consisting of a 5′-NG-3′ PAM-recognizing variant of...

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Bibliographic Details
Main Authors: Yi Yun Tan, Yin Yin Liew, Rachelle R. Q. Lee, Baptiste Castel, Nga Man Chan, Wei-Lin Wan, Yizhong Zhang, Donghui Hu, Persis Chan, Sang-Tae Kim, Eunyoung Chae
Format: Article
Language:English
Published: The American Phytopathological Society 2025-01-01
Series:Molecular Plant-Microbe Interactions
Subjects:
adenine deaminase
CRISPR/Cas9
genome editing
multigene editing
NLR
plant biotechnology
Online Access:https://apsjournals.apsnet.org/doi/10.1094/MPMI-10-24-0127-TA
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https://apsjournals.apsnet.org/doi/10.1094/MPMI-10-24-0127-TA

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