miR-375 Promotes Pancreatic Differentiation In Vitro by Affecting Different Target Genes at Different Stages
Human embryonic stem cells (hESCs) possess the ability to differentiate into insulin-producing cells (IPCs), which can be used to treat type I diabetes. miR-375 is an essential transcriptional regulator in the development and maturation of the pancreas. In this study, we optimized a protocol to diff...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/6642983 |
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| author | Zhenyu Lu Jing Wang Xu Wang Zhiying Li Dan Niu Min Wang Jinzhu Xiang Yongli Yue Yajuan Xia Xueling Li |
| author_facet | Zhenyu Lu Jing Wang Xu Wang Zhiying Li Dan Niu Min Wang Jinzhu Xiang Yongli Yue Yajuan Xia Xueling Li |
| author_sort | Zhenyu Lu |
| collection | DOAJ |
| description | Human embryonic stem cells (hESCs) possess the ability to differentiate into insulin-producing cells (IPCs), which can be used to treat type I diabetes. miR-375 is an essential transcriptional regulator in the development and maturation of the pancreas. In this study, we optimized a protocol to differentiate hESCs into IPCs and successfully obtained IPCs. Then, we performed overexpression and inhibition experiments of miR-375 on cells at different stages of differentiation and performed RNA-seq. The results showed that the expression of miR-375 fluctuated during hESC differentiation and was affected by miR-375 mimics and inhibitors. miR-375 influences global gene expression and the target genes of miR-375. The overexpression of miR-375 can cause changes in multiple signaling pathways during pancreatic development. miR-375 is a major participant in the differentiation of pancreatic β-cells through different target genes at different stages. This study provides new ideas for further investigation of how microRNAs affect cell fate and gene transcription. |
| format | Article |
| id | doaj-art-c9ae488f8a234f8999f9abeb03ec9176 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-c9ae488f8a234f8999f9abeb03ec91762025-02-03T01:25:26ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/66429836642983miR-375 Promotes Pancreatic Differentiation In Vitro by Affecting Different Target Genes at Different StagesZhenyu Lu0Jing Wang1Xu Wang2Zhiying Li3Dan Niu4Min Wang5Jinzhu Xiang6Yongli Yue7Yajuan Xia8Xueling Li9State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, ChinaInner Mongolia Center for Disease Control and Prevention, Hohhot, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, ChinaHuman embryonic stem cells (hESCs) possess the ability to differentiate into insulin-producing cells (IPCs), which can be used to treat type I diabetes. miR-375 is an essential transcriptional regulator in the development and maturation of the pancreas. In this study, we optimized a protocol to differentiate hESCs into IPCs and successfully obtained IPCs. Then, we performed overexpression and inhibition experiments of miR-375 on cells at different stages of differentiation and performed RNA-seq. The results showed that the expression of miR-375 fluctuated during hESC differentiation and was affected by miR-375 mimics and inhibitors. miR-375 influences global gene expression and the target genes of miR-375. The overexpression of miR-375 can cause changes in multiple signaling pathways during pancreatic development. miR-375 is a major participant in the differentiation of pancreatic β-cells through different target genes at different stages. This study provides new ideas for further investigation of how microRNAs affect cell fate and gene transcription.http://dx.doi.org/10.1155/2021/6642983 |
| spellingShingle | Zhenyu Lu Jing Wang Xu Wang Zhiying Li Dan Niu Min Wang Jinzhu Xiang Yongli Yue Yajuan Xia Xueling Li miR-375 Promotes Pancreatic Differentiation In Vitro by Affecting Different Target Genes at Different Stages Stem Cells International |
| title | miR-375 Promotes Pancreatic Differentiation In Vitro by Affecting Different Target Genes at Different Stages |
| title_full | miR-375 Promotes Pancreatic Differentiation In Vitro by Affecting Different Target Genes at Different Stages |
| title_fullStr | miR-375 Promotes Pancreatic Differentiation In Vitro by Affecting Different Target Genes at Different Stages |
| title_full_unstemmed | miR-375 Promotes Pancreatic Differentiation In Vitro by Affecting Different Target Genes at Different Stages |
| title_short | miR-375 Promotes Pancreatic Differentiation In Vitro by Affecting Different Target Genes at Different Stages |
| title_sort | mir 375 promotes pancreatic differentiation in vitro by affecting different target genes at different stages |
| url | http://dx.doi.org/10.1155/2021/6642983 |
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