Analysis of Polymorphisms in the Lactotransferrin Gene Promoter and Dental Caries
Regarding host aspects, there has been strong evidence for a genetic component in the etiology of caries. The salivary protein lactotransferrin (LTF) exhibits antibacterial activity, but there is no study investigating the association of polymorphisms in the promoter region of LTF gene with caries....
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2011/571726 |
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| author | João Armando Brancher Giovana Daniela Pecharki Andrea Duarte Doetzer Kamilla Gabriella dos Santos Medeiros Carlos Alberto Cordeiro Júnior Vanessa Santos Sotomaior Peter Bauer Paula Cristina Trevilatto |
| author_facet | João Armando Brancher Giovana Daniela Pecharki Andrea Duarte Doetzer Kamilla Gabriella dos Santos Medeiros Carlos Alberto Cordeiro Júnior Vanessa Santos Sotomaior Peter Bauer Paula Cristina Trevilatto |
| author_sort | João Armando Brancher |
| collection | DOAJ |
| description | Regarding host aspects, there has been strong evidence for a genetic component in the etiology of caries. The salivary protein lactotransferrin (LTF) exhibits antibacterial activity, but there is no study investigating the association of polymorphisms in the promoter region of LTF gene with caries. The objective of this study was firstly to search the promoter region of the human LTF gene for variations and, if existent, to investigate the association of the identified polymorphisms with dental caries in 12-year-old students. From 687 unrelated, 12-year-old, both sex students, 50 individuals were selected and divided into two groups of extreme phenotypes according to caries experience: 25 students without (DMFT = 0) and 25 with caries experience (DMFT ≥ 4). The selection of individuals with extreme phenotypes augments the chances to find gene variations which could be associated with such phenotypes. LTF gene-putative promoter region (+39 to −1143) of the selected 50 individuals was analyzed by high-resolution melting technique. Fifteen students, 8 without (DMFT = 0) and 7 with caries experience (mean DMFT = 6.28), presented deviations of the pattern curve suggestive of gene variations and were sequenced. However, no polymorphisms were identified in the putative promoter region of the LTF gene. |
| format | Article |
| id | doaj-art-c996694f419040b29220d72816870fb9 |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-c996694f419040b29220d72816870fb92025-02-03T07:25:32ZengWileyInternational Journal of Dentistry1687-87281687-87362011-01-01201110.1155/2011/571726571726Analysis of Polymorphisms in the Lactotransferrin Gene Promoter and Dental CariesJoão Armando Brancher0Giovana Daniela Pecharki1Andrea Duarte Doetzer2Kamilla Gabriella dos Santos Medeiros3Carlos Alberto Cordeiro Júnior4Vanessa Santos Sotomaior5Peter Bauer6Paula Cristina Trevilatto7Center for Health and Biological Sciences, Pontifical Catholic University of Paraná (PUCPR), Imaculada Conceição Street 1155, 80215-901 Curitiba, PR, BrazilCenter for Health and Biological Sciences, Pontifical Catholic University of Paraná (PUCPR), Imaculada Conceição Street 1155, 80215-901 Curitiba, PR, BrazilCenter for Health and Biological Sciences, Pontifical Catholic University of Paraná (PUCPR), Imaculada Conceição Street 1155, 80215-901 Curitiba, PR, BrazilCenter for Health and Biological Sciences, Pontifical Catholic University of Paraná (PUCPR), Imaculada Conceição Street 1155, 80215-901 Curitiba, PR, BrazilCenter for Health and Biological Sciences, Pontifical Catholic University of Paraná (PUCPR), Imaculada Conceição Street 1155, 80215-901 Curitiba, PR, BrazilCenter for Health and Biological Sciences, Pontifical Catholic University of Paraná (PUCPR), Imaculada Conceição Street 1155, 80215-901 Curitiba, PR, BrazilDepartment of Medical Genetics, University of Tübingen, Calwerstrasse 7, Tübingen D 72076, GermanyCenter for Health and Biological Sciences, Pontifical Catholic University of Paraná (PUCPR), Imaculada Conceição Street 1155, 80215-901 Curitiba, PR, BrazilRegarding host aspects, there has been strong evidence for a genetic component in the etiology of caries. The salivary protein lactotransferrin (LTF) exhibits antibacterial activity, but there is no study investigating the association of polymorphisms in the promoter region of LTF gene with caries. The objective of this study was firstly to search the promoter region of the human LTF gene for variations and, if existent, to investigate the association of the identified polymorphisms with dental caries in 12-year-old students. From 687 unrelated, 12-year-old, both sex students, 50 individuals were selected and divided into two groups of extreme phenotypes according to caries experience: 25 students without (DMFT = 0) and 25 with caries experience (DMFT ≥ 4). The selection of individuals with extreme phenotypes augments the chances to find gene variations which could be associated with such phenotypes. LTF gene-putative promoter region (+39 to −1143) of the selected 50 individuals was analyzed by high-resolution melting technique. Fifteen students, 8 without (DMFT = 0) and 7 with caries experience (mean DMFT = 6.28), presented deviations of the pattern curve suggestive of gene variations and were sequenced. However, no polymorphisms were identified in the putative promoter region of the LTF gene.http://dx.doi.org/10.1155/2011/571726 |
| spellingShingle | João Armando Brancher Giovana Daniela Pecharki Andrea Duarte Doetzer Kamilla Gabriella dos Santos Medeiros Carlos Alberto Cordeiro Júnior Vanessa Santos Sotomaior Peter Bauer Paula Cristina Trevilatto Analysis of Polymorphisms in the Lactotransferrin Gene Promoter and Dental Caries International Journal of Dentistry |
| title | Analysis of Polymorphisms in the Lactotransferrin Gene Promoter and Dental Caries |
| title_full | Analysis of Polymorphisms in the Lactotransferrin Gene Promoter and Dental Caries |
| title_fullStr | Analysis of Polymorphisms in the Lactotransferrin Gene Promoter and Dental Caries |
| title_full_unstemmed | Analysis of Polymorphisms in the Lactotransferrin Gene Promoter and Dental Caries |
| title_short | Analysis of Polymorphisms in the Lactotransferrin Gene Promoter and Dental Caries |
| title_sort | analysis of polymorphisms in the lactotransferrin gene promoter and dental caries |
| url | http://dx.doi.org/10.1155/2011/571726 |
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