Time Course of the Development of Nonalcoholic Fatty Liver Disease in the Otsuka Long-Evans Tokushima Fatty Rat
Nonalcoholic fatty liver disease (NAFLD) is considered a hepatic manifestation of metabolic syndrome. In this study, we investigated histological and biochemical changes in NAFLD and the gene expression involving de novo lipogenesis in Otsuka Long-Evans Tokushima fatty (OLETF) rats. We used OLETF ra...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2013/342648 |
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| author | Yi-Sun Song Cheng-Hu Fang Byung-Im So Jun-Young Park Yonggu Lee Jeong Hun Shin Dae Won Jun Hyuck Kim Kyung-Soo Kim |
| author_facet | Yi-Sun Song Cheng-Hu Fang Byung-Im So Jun-Young Park Yonggu Lee Jeong Hun Shin Dae Won Jun Hyuck Kim Kyung-Soo Kim |
| author_sort | Yi-Sun Song |
| collection | DOAJ |
| description | Nonalcoholic fatty liver disease (NAFLD) is considered a hepatic manifestation of metabolic syndrome. In this study, we investigated histological and biochemical changes in NAFLD and the gene expression involving de novo lipogenesis in Otsuka Long-Evans Tokushima fatty (OLETF) rats. We used OLETF rats and Long-Evans Tokushima Otsuka (LETO) rats as animal models of NAFLD and as controls, respectively. Consistent observations were made at 4-week intervals up to 50 weeks of age, and all rats were fed ad libitum with standard food. Biochemical and histological changes were observed, and gene expression involved in de novo lipogenesis was measured using real-time polymerase chain reactions. As a results hepatic micro- and macrovesicular steatosis and hepatocyte ballooning were evident in the OLETF rats at 22–38 weeks of age but disappeared after 42 weeks; no fibrosis or collagen deposition was observed. Gene expression involved in de novo lipogenesis followed a pattern similar to that of the histological changes. In conclusion, in the absence of dietary manipulation, hepatic steatosis in OLETF rats is evident at 22–38 weeks and declines after 42 weeks. Therefore, OLETF rats at 22–38 weeks are recommended as animal models of hepatic steatosis. |
| format | Article |
| id | doaj-art-c9923f5e2aee46f99f1f7cab9999fa1a |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-c9923f5e2aee46f99f1f7cab9999fa1a2025-02-03T05:48:21ZengWileyGastroenterology Research and Practice1687-61211687-630X2013-01-01201310.1155/2013/342648342648Time Course of the Development of Nonalcoholic Fatty Liver Disease in the Otsuka Long-Evans Tokushima Fatty RatYi-Sun Song0Cheng-Hu Fang1Byung-Im So2Jun-Young Park3Yonggu Lee4Jeong Hun Shin5Dae Won Jun6Hyuck Kim7Kyung-Soo Kim8Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-792, Republic of KoreaDepartment of Internal Medicine, Hanyang University College of Medicine, 17 Haengdang-dong, Sungdong-ku, Seoul 133-792, Republic of KoreaGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-792, Republic of KoreaGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-792, Republic of KoreaDepartment of Internal Medicine, Hanyang University College of Medicine, 17 Haengdang-dong, Sungdong-ku, Seoul 133-792, Republic of KoreaDepartment of Internal Medicine, Hanyang University College of Medicine, 17 Haengdang-dong, Sungdong-ku, Seoul 133-792, Republic of KoreaDepartment of Internal Medicine, Hanyang University College of Medicine, 17 Haengdang-dong, Sungdong-ku, Seoul 133-792, Republic of KoreaDepartment of Thoracic and Cardiovascular Surgery, Hanyang University College of Medicine, Seoul 133-792, Republic of KoreaGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-792, Republic of KoreaNonalcoholic fatty liver disease (NAFLD) is considered a hepatic manifestation of metabolic syndrome. In this study, we investigated histological and biochemical changes in NAFLD and the gene expression involving de novo lipogenesis in Otsuka Long-Evans Tokushima fatty (OLETF) rats. We used OLETF rats and Long-Evans Tokushima Otsuka (LETO) rats as animal models of NAFLD and as controls, respectively. Consistent observations were made at 4-week intervals up to 50 weeks of age, and all rats were fed ad libitum with standard food. Biochemical and histological changes were observed, and gene expression involved in de novo lipogenesis was measured using real-time polymerase chain reactions. As a results hepatic micro- and macrovesicular steatosis and hepatocyte ballooning were evident in the OLETF rats at 22–38 weeks of age but disappeared after 42 weeks; no fibrosis or collagen deposition was observed. Gene expression involved in de novo lipogenesis followed a pattern similar to that of the histological changes. In conclusion, in the absence of dietary manipulation, hepatic steatosis in OLETF rats is evident at 22–38 weeks and declines after 42 weeks. Therefore, OLETF rats at 22–38 weeks are recommended as animal models of hepatic steatosis.http://dx.doi.org/10.1155/2013/342648 |
| spellingShingle | Yi-Sun Song Cheng-Hu Fang Byung-Im So Jun-Young Park Yonggu Lee Jeong Hun Shin Dae Won Jun Hyuck Kim Kyung-Soo Kim Time Course of the Development of Nonalcoholic Fatty Liver Disease in the Otsuka Long-Evans Tokushima Fatty Rat Gastroenterology Research and Practice |
| title | Time Course of the Development of Nonalcoholic Fatty Liver Disease in the Otsuka Long-Evans Tokushima Fatty Rat |
| title_full | Time Course of the Development of Nonalcoholic Fatty Liver Disease in the Otsuka Long-Evans Tokushima Fatty Rat |
| title_fullStr | Time Course of the Development of Nonalcoholic Fatty Liver Disease in the Otsuka Long-Evans Tokushima Fatty Rat |
| title_full_unstemmed | Time Course of the Development of Nonalcoholic Fatty Liver Disease in the Otsuka Long-Evans Tokushima Fatty Rat |
| title_short | Time Course of the Development of Nonalcoholic Fatty Liver Disease in the Otsuka Long-Evans Tokushima Fatty Rat |
| title_sort | time course of the development of nonalcoholic fatty liver disease in the otsuka long evans tokushima fatty rat |
| url | http://dx.doi.org/10.1155/2013/342648 |
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