Development and Application of <i>Lactobacillus plantarum</i> PSCPL13 Probiotics in Olive Flounder (<i>Paralichthys olivaceus</i>) Farming
Aquaculture has grown significantly, contributing to global food security and sustainability; however, intensified fish farming has increased disease susceptibility and antibiotic resistance. This study assessed the probiotic potential of <i>Lactobacillus plantarum</i> PSCPL13 (hereafter...
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2025-01-01
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| author | Muhammad Aleem Abbas Hae-Jung Kim Ga-Yeong Lee Hae-Yeon Cho Syed Al Jawad Sayem Eon-Bee Lee Seung-Jin Lee Seung-Chun Park |
| author_facet | Muhammad Aleem Abbas Hae-Jung Kim Ga-Yeong Lee Hae-Yeon Cho Syed Al Jawad Sayem Eon-Bee Lee Seung-Jin Lee Seung-Chun Park |
| author_sort | Muhammad Aleem Abbas |
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| description | Aquaculture has grown significantly, contributing to global food security and sustainability; however, intensified fish farming has increased disease susceptibility and antibiotic resistance. This study assessed the probiotic potential of <i>Lactobacillus plantarum</i> PSCPL13 (hereafter, PSCPL13), isolated from the intestines of Japanese eels, for enhancing the health of olive flounder. After screening 16 <i>Lactobacillus</i> isolates, PSCPL13 was selected because of its potential broad-spectrum antibacterial activity against many pathogens, such as <i>Vibrio</i> and <i>Edwardsiella</i>. This strain exhibited high acid and bile salt tolerance, which is crucial for intestinal survival. Molecular characterisation confirmed PSCPL13 to be <i>L. plantarum</i>. It was resistant to all tested antibiotics and exhibited significant enzyme activity. In vivo assays in olive flounder revealed that the body weight and length of the fish were significantly higher, while the prevalence of pathogens was lower in their gut microbiota. Regarding haematological parameters, the treated groups exhibited lower eosinophil counts and higher IgM levels, suggesting enhancement of the immune system. These findings indicate that PSCPL13 is a potential probiotic that can be used in aquaculture for naturally improving fish health, growth and immunity, in addition to combating antibiotic resistance and reducing environmental impacts. These findings not only highlight the potential of PSCPL13 in sustainable aquaculture but also provide a foundation for the development of future probiotics. |
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| institution | Kabale University |
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| language | English |
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| series | Microorganisms |
| spelling | doaj-art-c981c92ca59f4a41b0caf48d7728d2a42025-01-24T13:42:28ZengMDPI AGMicroorganisms2076-26072025-01-011316110.3390/microorganisms13010061Development and Application of <i>Lactobacillus plantarum</i> PSCPL13 Probiotics in Olive Flounder (<i>Paralichthys olivaceus</i>) FarmingMuhammad Aleem Abbas0Hae-Jung Kim1Ga-Yeong Lee2Hae-Yeon Cho3Syed Al Jawad Sayem4Eon-Bee Lee5Seung-Jin Lee6Seung-Chun Park7Laboratory of Veterinary Pharmacokinetics, Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaLaboratory of Veterinary Pharmacokinetics, Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaLaboratory of Veterinary Pharmacokinetics, Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaLaboratory of Veterinary Pharmacokinetics, Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaLaboratory of Veterinary Pharmacokinetics, Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Aquatic Life Medicine, Pukyong National University, Busan 48513, Republic of KoreaDevelopmental and Reproductive Toxicology Research Group, Korea Institute of Toxicology, Daejeon 34114, Republic of KoreaLaboratory of Veterinary Pharmacokinetics, Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaAquaculture has grown significantly, contributing to global food security and sustainability; however, intensified fish farming has increased disease susceptibility and antibiotic resistance. This study assessed the probiotic potential of <i>Lactobacillus plantarum</i> PSCPL13 (hereafter, PSCPL13), isolated from the intestines of Japanese eels, for enhancing the health of olive flounder. After screening 16 <i>Lactobacillus</i> isolates, PSCPL13 was selected because of its potential broad-spectrum antibacterial activity against many pathogens, such as <i>Vibrio</i> and <i>Edwardsiella</i>. This strain exhibited high acid and bile salt tolerance, which is crucial for intestinal survival. Molecular characterisation confirmed PSCPL13 to be <i>L. plantarum</i>. It was resistant to all tested antibiotics and exhibited significant enzyme activity. In vivo assays in olive flounder revealed that the body weight and length of the fish were significantly higher, while the prevalence of pathogens was lower in their gut microbiota. Regarding haematological parameters, the treated groups exhibited lower eosinophil counts and higher IgM levels, suggesting enhancement of the immune system. These findings indicate that PSCPL13 is a potential probiotic that can be used in aquaculture for naturally improving fish health, growth and immunity, in addition to combating antibiotic resistance and reducing environmental impacts. These findings not only highlight the potential of PSCPL13 in sustainable aquaculture but also provide a foundation for the development of future probiotics.https://www.mdpi.com/2076-2607/13/1/61fish probioticaquaculturefish gut microbiotafish nutritionaquatic microorganisms<i>Lactobacillus plantarum</i> |
| spellingShingle | Muhammad Aleem Abbas Hae-Jung Kim Ga-Yeong Lee Hae-Yeon Cho Syed Al Jawad Sayem Eon-Bee Lee Seung-Jin Lee Seung-Chun Park Development and Application of <i>Lactobacillus plantarum</i> PSCPL13 Probiotics in Olive Flounder (<i>Paralichthys olivaceus</i>) Farming Microorganisms fish probiotic aquaculture fish gut microbiota fish nutrition aquatic microorganisms <i>Lactobacillus plantarum</i> |
| title | Development and Application of <i>Lactobacillus plantarum</i> PSCPL13 Probiotics in Olive Flounder (<i>Paralichthys olivaceus</i>) Farming |
| title_full | Development and Application of <i>Lactobacillus plantarum</i> PSCPL13 Probiotics in Olive Flounder (<i>Paralichthys olivaceus</i>) Farming |
| title_fullStr | Development and Application of <i>Lactobacillus plantarum</i> PSCPL13 Probiotics in Olive Flounder (<i>Paralichthys olivaceus</i>) Farming |
| title_full_unstemmed | Development and Application of <i>Lactobacillus plantarum</i> PSCPL13 Probiotics in Olive Flounder (<i>Paralichthys olivaceus</i>) Farming |
| title_short | Development and Application of <i>Lactobacillus plantarum</i> PSCPL13 Probiotics in Olive Flounder (<i>Paralichthys olivaceus</i>) Farming |
| title_sort | development and application of i lactobacillus plantarum i pscpl13 probiotics in olive flounder i paralichthys olivaceus i farming |
| topic | fish probiotic aquaculture fish gut microbiota fish nutrition aquatic microorganisms <i>Lactobacillus plantarum</i> |
| url | https://www.mdpi.com/2076-2607/13/1/61 |
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