A diagnostic host-specific transcriptome response for Mycoplasma pneumoniae pneumonia to guide pediatric patient treatment
Abstract Mycoplasma pneumoniae causes atypical pneumonia in children and young adults. Its lack of a cell wall makes it resistant to beta-lactams, which are the first-line treatment for typical pneumonia. Current diagnostic tests are time-consuming and have low specificity, leading clinicians to adm...
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2025-01-01
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| Online Access: | https://doi.org/10.1038/s41467-025-55932-9 |
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| author | Sandra Viz-Lasheras Alberto Gómez-Carballa Xabier Bello Irene Rivero-Calle Ana Isabel Dacosta Myrsini Kaforou Dominic Habgood-Coote Aubrey J. Cunnington Marieke Emonts Jethro A. Herberg Victoria J. Wright Enitan D. Carrol Stephane C. Paulus Werner Zenz Daniela S. Kohlfürst Nina Schweintzger Michiel Van der Flier Ronald de Groot Luregn J. Schlapbach Philipp Agyeman Andrew J. Pollard Colin Fink Taco T. Kuijpers Suzanne Anderson Ulrich Von Both Marko Pokorn Dace Zavadska María Tsolia Henriëtte A. Moll Clementien Vermont Michael Levin Federico Martinón-Torres Antonio Salas On behalf of EUCLIDS, PERFORM, and DIAMONDS consortia |
| author_facet | Sandra Viz-Lasheras Alberto Gómez-Carballa Xabier Bello Irene Rivero-Calle Ana Isabel Dacosta Myrsini Kaforou Dominic Habgood-Coote Aubrey J. Cunnington Marieke Emonts Jethro A. Herberg Victoria J. Wright Enitan D. Carrol Stephane C. Paulus Werner Zenz Daniela S. Kohlfürst Nina Schweintzger Michiel Van der Flier Ronald de Groot Luregn J. Schlapbach Philipp Agyeman Andrew J. Pollard Colin Fink Taco T. Kuijpers Suzanne Anderson Ulrich Von Both Marko Pokorn Dace Zavadska María Tsolia Henriëtte A. Moll Clementien Vermont Michael Levin Federico Martinón-Torres Antonio Salas On behalf of EUCLIDS, PERFORM, and DIAMONDS consortia |
| author_sort | Sandra Viz-Lasheras |
| collection | DOAJ |
| description | Abstract Mycoplasma pneumoniae causes atypical pneumonia in children and young adults. Its lack of a cell wall makes it resistant to beta-lactams, which are the first-line treatment for typical pneumonia. Current diagnostic tests are time-consuming and have low specificity, leading clinicians to administer empirical antibiotics. Using a LASSO regression simulation approach and blood microarray data from 107 children with pneumonia (including 30 M. pneumoniae) we identify eight different transcriptomic signatures, ranging from 3-10 transcripts, that differentiate mycoplasma pneumonia from other bacterial/viral pneumonias with high accuracy (AUC: 0.84–0.95). Additionally, we demonstrate that existing signatures for broadly distinguishing viral/bacterial infections and viral/bacterial pneumonias are ineffective in distinguishing M. pneumoniae from viral pneumonia. The new signatures are successfully validated in an independent RNAseq cohort of children with pneumonia, demonstrating their robustness. The high sensibility of these signatures presents a valuable opportunity to guide the treatment and management of M. pneumoniae pneumonia patients. |
| format | Article |
| id | doaj-art-c97ca7e6166e4e949956008ec6947f56 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-c97ca7e6166e4e949956008ec6947f562025-01-19T12:31:44ZengNature PortfolioNature Communications2041-17232025-01-0116111310.1038/s41467-025-55932-9A diagnostic host-specific transcriptome response for Mycoplasma pneumoniae pneumonia to guide pediatric patient treatmentSandra Viz-Lasheras0Alberto Gómez-Carballa1Xabier Bello2Irene Rivero-Calle3Ana Isabel Dacosta4Myrsini Kaforou5Dominic Habgood-Coote6Aubrey J. Cunnington7Marieke Emonts8Jethro A. Herberg9Victoria J. Wright10Enitan D. Carrol11Stephane C. Paulus12Werner Zenz13Daniela S. Kohlfürst14Nina Schweintzger15Michiel Van der Flier16Ronald de Groot17Luregn J. Schlapbach18Philipp Agyeman19Andrew J. Pollard20Colin Fink21Taco T. Kuijpers22Suzanne Anderson23Ulrich Von Both24Marko Pokorn25Dace Zavadska26María Tsolia27Henriëtte A. Moll28Clementien Vermont29Michael Levin30Federico Martinón-Torres31Antonio Salas32On behalf of EUCLIDS, PERFORM, and DIAMONDS consortiaUnidade de Xenética, Instituto de Ciencias Forenses, Facultade de Medicina, Universidade de Santiago de CompostelaUnidade de Xenética, Instituto de Ciencias Forenses, Facultade de Medicina, Universidade de Santiago de CompostelaUnidade de Xenética, Instituto de Ciencias Forenses, Facultade de Medicina, Universidade de Santiago de CompostelaGenetics, Vaccines and Infections Research Group (GenViP), Instituto de Investigación Sanitaria de Santiago (IDIS), 15706 Hospital Clínico Universitario de Santiago (SERGAS)Genetics, Vaccines and Infections Research Group (GenViP), Instituto de Investigación Sanitaria de Santiago (IDIS), 15706 Hospital Clínico Universitario de Santiago (SERGAS)Department of Infectious Disease, Imperial College LondonDepartment of Infectious Disease, Imperial College LondonDepartment of Infectious Disease, Imperial College LondonPaediatric Immunology, Infectious Diseases & Allergy, Great North Children’s Hospital, Newcastle upon Tyne Hospitals NHS Foundation TrustDepartment of Infectious Disease, Imperial College LondonDepartment of Infectious Disease, Imperial College LondonDepartment of Infectious Diseases, Alder Hey Children’s NHS Foundation TrustDepartment of Paediatrics, University of Oxford and the NIHR Oxford Biomedical Research CentreDepartment of General Paediatrics, Medical University of Graz, GrazDepartment of General Paediatrics, Medical University of Graz, GrazDepartment of General Paediatrics, Medical University of Graz, GrazPediatric Infectious Diseases and Immunology, Wilhelmina Children’s Hospital, University Medical Center UtrechtPediatric Infectious Diseases and Immunology, Amalia Children’s Hospital, and Section Pediatric Infectious Diseases, Laboratory of Medical Immunology, Department of Laboratory Medicine, Radboud Institute for Molecular Life Sciences, Radboud University Medical CenterDepartment of Intensive Care and Neonatology, and Children’s Research Center, University Children’s Hospital Zürich, University of ZürichDepartment of Pediatrics, Inselspital, Bern University Hospital, University of BernDepartment of Paediatrics, University of Oxford and the NIHR Oxford Biomedical Research CentreMicropathology Ltd, University of WarwickDivision of Pediatric Immunology, Rheumatology and Infectious diseases, Emma Children’s Hospital, Amsterdam Univiersyt Medical Center (Amsterdam UMC)Medical Research Council Unit at the London School of Hygene & Tropical MedicineInfectious Diseases, Department of Pediatrics, Dr von Hauner Children’s Hospital, University Hospital, LMU MunichDivision of Paediatrics, University Medical Centre Ljubljana and Medical Faculty, University of LjubljanaChildren’s Clinical University Hospital, Rīga Stradins UniversitySecond Department of Paediatrics, National and Kapodistrian University of Athens (NKUA), School of Medicine, Panagiotis & Aglaia, Kyriakou Children’s HospitalDepartment of Paediatric Infectious Diseases and Immunology, Erasmus MC Sophia Children’s HospitalDepartment of Paediatric Infectious Diseases and Immunology, Erasmus MC Sophia Children’s HospitalDepartment of Infectious Disease, Imperial College LondonGenetics, Vaccines and Infections Research Group (GenViP), Instituto de Investigación Sanitaria de Santiago (IDIS), 15706 Hospital Clínico Universitario de Santiago (SERGAS)Unidade de Xenética, Instituto de Ciencias Forenses, Facultade de Medicina, Universidade de Santiago de CompostelaAbstract Mycoplasma pneumoniae causes atypical pneumonia in children and young adults. Its lack of a cell wall makes it resistant to beta-lactams, which are the first-line treatment for typical pneumonia. Current diagnostic tests are time-consuming and have low specificity, leading clinicians to administer empirical antibiotics. Using a LASSO regression simulation approach and blood microarray data from 107 children with pneumonia (including 30 M. pneumoniae) we identify eight different transcriptomic signatures, ranging from 3-10 transcripts, that differentiate mycoplasma pneumonia from other bacterial/viral pneumonias with high accuracy (AUC: 0.84–0.95). Additionally, we demonstrate that existing signatures for broadly distinguishing viral/bacterial infections and viral/bacterial pneumonias are ineffective in distinguishing M. pneumoniae from viral pneumonia. The new signatures are successfully validated in an independent RNAseq cohort of children with pneumonia, demonstrating their robustness. The high sensibility of these signatures presents a valuable opportunity to guide the treatment and management of M. pneumoniae pneumonia patients.https://doi.org/10.1038/s41467-025-55932-9 |
| spellingShingle | Sandra Viz-Lasheras Alberto Gómez-Carballa Xabier Bello Irene Rivero-Calle Ana Isabel Dacosta Myrsini Kaforou Dominic Habgood-Coote Aubrey J. Cunnington Marieke Emonts Jethro A. Herberg Victoria J. Wright Enitan D. Carrol Stephane C. Paulus Werner Zenz Daniela S. Kohlfürst Nina Schweintzger Michiel Van der Flier Ronald de Groot Luregn J. Schlapbach Philipp Agyeman Andrew J. Pollard Colin Fink Taco T. Kuijpers Suzanne Anderson Ulrich Von Both Marko Pokorn Dace Zavadska María Tsolia Henriëtte A. Moll Clementien Vermont Michael Levin Federico Martinón-Torres Antonio Salas On behalf of EUCLIDS, PERFORM, and DIAMONDS consortia A diagnostic host-specific transcriptome response for Mycoplasma pneumoniae pneumonia to guide pediatric patient treatment Nature Communications |
| title | A diagnostic host-specific transcriptome response for Mycoplasma pneumoniae pneumonia to guide pediatric patient treatment |
| title_full | A diagnostic host-specific transcriptome response for Mycoplasma pneumoniae pneumonia to guide pediatric patient treatment |
| title_fullStr | A diagnostic host-specific transcriptome response for Mycoplasma pneumoniae pneumonia to guide pediatric patient treatment |
| title_full_unstemmed | A diagnostic host-specific transcriptome response for Mycoplasma pneumoniae pneumonia to guide pediatric patient treatment |
| title_short | A diagnostic host-specific transcriptome response for Mycoplasma pneumoniae pneumonia to guide pediatric patient treatment |
| title_sort | diagnostic host specific transcriptome response for mycoplasma pneumoniae pneumonia to guide pediatric patient treatment |
| url | https://doi.org/10.1038/s41467-025-55932-9 |
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