Meningococcal Antigen Typing System Development and Application to the Evaluation of Effectiveness of Meningococcal B Vaccine and Possible Use for Other Purposes
Development of the 4-component meningococcal serogroup B vaccine (4CMenB) has required new assays for the reliable evaluation of the expression and cross-reactivity of those specific antigen variants that are predicted to be targeted by bactericidal antibodies elicited by the vaccine in different is...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/353461 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561114798358528 |
|---|---|
| author | Alexander Domnich Roberto Gasparini Daniela Amicizia Giuseppe Boccadifuoco Marzia Monica Giuliani Donatella Panatto |
| author_facet | Alexander Domnich Roberto Gasparini Daniela Amicizia Giuseppe Boccadifuoco Marzia Monica Giuliani Donatella Panatto |
| author_sort | Alexander Domnich |
| collection | DOAJ |
| description | Development of the 4-component meningococcal serogroup B vaccine (4CMenB) has required new assays for the reliable evaluation of the expression and cross-reactivity of those specific antigen variants that are predicted to be targeted by bactericidal antibodies elicited by the vaccine in different isolates. Existing laboratory techniques, such as multilocus sequence typing, are poorly suited to this purpose, since they do not provide information on the contribution of single vaccine components and therefore cannot be applied to estimate the potential coverage of the multicomponent vaccine. The hSBA, the only correlate of protection against invasive meningococcal disease accepted thus far, cannot conveniently be used to test large number of strains. To overcome these issues, the meningococcal antigen typing system (MATS) has been specifically developed in order to predict 4CMenB coverage of individual meningococcus serogroup B strains. To date, MATS has proved advantageous for several reasons, including its ability to assess both qualitative and quantitative aspects of surface antigens of single strains in a highly reproducible, rapid, and resource-saving manner, while its shortcomings include a possible underestimation of 4CMenB coverage and the use of pooled sera to calculate the positive bactericidal threshold. This paper provides an overview of MATS development and its field application. |
| format | Article |
| id | doaj-art-c974fb9ff75441b9a21518c7586c943e |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-c974fb9ff75441b9a21518c7586c943e2025-02-03T01:25:56ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/353461353461Meningococcal Antigen Typing System Development and Application to the Evaluation of Effectiveness of Meningococcal B Vaccine and Possible Use for Other PurposesAlexander Domnich0Roberto Gasparini1Daniela Amicizia2Giuseppe Boccadifuoco3Marzia Monica Giuliani4Donatella Panatto5Department of Health Sciences, University of Genoa, Via Pastore 1, 16132 Genoa, ItalyDepartment of Health Sciences, University of Genoa, Via Pastore 1, 16132 Genoa, ItalyDepartment of Health Sciences, University of Genoa, Via Pastore 1, 16132 Genoa, ItalyNovartis Vaccines, Via Fiorentina 1, 53100 Siena, ItalyNovartis Vaccines, Via Fiorentina 1, 53100 Siena, ItalyDepartment of Health Sciences, University of Genoa, Via Pastore 1, 16132 Genoa, ItalyDevelopment of the 4-component meningococcal serogroup B vaccine (4CMenB) has required new assays for the reliable evaluation of the expression and cross-reactivity of those specific antigen variants that are predicted to be targeted by bactericidal antibodies elicited by the vaccine in different isolates. Existing laboratory techniques, such as multilocus sequence typing, are poorly suited to this purpose, since they do not provide information on the contribution of single vaccine components and therefore cannot be applied to estimate the potential coverage of the multicomponent vaccine. The hSBA, the only correlate of protection against invasive meningococcal disease accepted thus far, cannot conveniently be used to test large number of strains. To overcome these issues, the meningococcal antigen typing system (MATS) has been specifically developed in order to predict 4CMenB coverage of individual meningococcus serogroup B strains. To date, MATS has proved advantageous for several reasons, including its ability to assess both qualitative and quantitative aspects of surface antigens of single strains in a highly reproducible, rapid, and resource-saving manner, while its shortcomings include a possible underestimation of 4CMenB coverage and the use of pooled sera to calculate the positive bactericidal threshold. This paper provides an overview of MATS development and its field application.http://dx.doi.org/10.1155/2015/353461 |
| spellingShingle | Alexander Domnich Roberto Gasparini Daniela Amicizia Giuseppe Boccadifuoco Marzia Monica Giuliani Donatella Panatto Meningococcal Antigen Typing System Development and Application to the Evaluation of Effectiveness of Meningococcal B Vaccine and Possible Use for Other Purposes Journal of Immunology Research |
| title | Meningococcal Antigen Typing System Development and Application to the Evaluation of Effectiveness of Meningococcal B Vaccine and Possible Use for Other Purposes |
| title_full | Meningococcal Antigen Typing System Development and Application to the Evaluation of Effectiveness of Meningococcal B Vaccine and Possible Use for Other Purposes |
| title_fullStr | Meningococcal Antigen Typing System Development and Application to the Evaluation of Effectiveness of Meningococcal B Vaccine and Possible Use for Other Purposes |
| title_full_unstemmed | Meningococcal Antigen Typing System Development and Application to the Evaluation of Effectiveness of Meningococcal B Vaccine and Possible Use for Other Purposes |
| title_short | Meningococcal Antigen Typing System Development and Application to the Evaluation of Effectiveness of Meningococcal B Vaccine and Possible Use for Other Purposes |
| title_sort | meningococcal antigen typing system development and application to the evaluation of effectiveness of meningococcal b vaccine and possible use for other purposes |
| url | http://dx.doi.org/10.1155/2015/353461 |
| work_keys_str_mv | AT alexanderdomnich meningococcalantigentypingsystemdevelopmentandapplicationtotheevaluationofeffectivenessofmeningococcalbvaccineandpossibleuseforotherpurposes AT robertogasparini meningococcalantigentypingsystemdevelopmentandapplicationtotheevaluationofeffectivenessofmeningococcalbvaccineandpossibleuseforotherpurposes AT danielaamicizia meningococcalantigentypingsystemdevelopmentandapplicationtotheevaluationofeffectivenessofmeningococcalbvaccineandpossibleuseforotherpurposes AT giuseppeboccadifuoco meningococcalantigentypingsystemdevelopmentandapplicationtotheevaluationofeffectivenessofmeningococcalbvaccineandpossibleuseforotherpurposes AT marziamonicagiuliani meningococcalantigentypingsystemdevelopmentandapplicationtotheevaluationofeffectivenessofmeningococcalbvaccineandpossibleuseforotherpurposes AT donatellapanatto meningococcalantigentypingsystemdevelopmentandapplicationtotheevaluationofeffectivenessofmeningococcalbvaccineandpossibleuseforotherpurposes |